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1、NEB内切酶产品具体Buffer活 性表NEBuffer Activity Chart for Restriction EnzymesApeKINEBuffer 3257510050No75BApolNEBuffer 3 + BSA1075100758050AAsciN EBuffer 4010101006537AfiliAselNEBuffer 3NR75100 ddNR6537BraioAsiSINEBuffer 4 + BSA501001001008037BRDoAvalNEBuffer 41075101008037ARH0AvailgheNEBuffer 45075101006537A
2、AvrilghoNEBuffer 4100100501008037BEnzyme topSupplied NEBufferQ/o inBl% inB2% inB3% inB4HeatInactiva.Incuba.Temp.DiluentBaeGINEBuffer 11007510506537ABaelNEBuffer 4 + BSA + SAM50100501006525ABamHINEBuffer 3 + BSA75100100dd100No37ABamHI-HFNEBuffer 41005010100No37A RiiQYBanI皿NEBuffer 4 + BSA501005010065
3、37ABanllEENEBuffer 410010501008037ABbsINEBuffer 210010025756537BBbvCINEBuffer 450100101008037ABbvlTNEBuffer 210010025756537BBed回NEBuffer 1 + BSA1005010506537ABceAINEBuffer 3 + BSA1001001001006537ABcgl回NEBuffer 3 + SAMNRNR100ddNR6537ABciVIcueNEBuffer 41005001006537CBellNEBuffer 350100100dd75No50ABfal
4、NEBuffer 47550101008037BBfuAInNEBuffer 3075100106550BBfuCINEBuffer 4 + BSA10050251008037BBgllNEBuffer 35075100506537BBglllEdNEBuffer 3107510010No37ABlplEdNEBuffer 45010010100No37ABmgBINEBuffer 3 + BSA025100106537CBmrINEBuffer 275100751006537BNew England Biolabs provides a color-coded 10X NEBuffer wi
5、th every restriction endonuclease to ensure 100% activity. To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer and approximate activity in the four standard NEBuffers for each enzyme. If BSA is supplied with an enzyme, it is included in all NEBuffe
6、r activity reactions. In addition, the table also shows recommended reaction temperature, heat inactivation temperature and recommended diluent buffer.Please click here for additional information about:Unique NEBuffer Activity | Double Digestion | Heat Inactivation | Activity at 37 | Diluent Buffers
7、 | Time-Saver Enzymes | High Fidelity (HF) Restriction EnzymesThis chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.l, 2008.Click for the Chart Legend, Icon DescriptionA|B|C|D|E|F|H|I|K|M|N|P|R|S|T|X|Z|EnzymeSupplied NEBuffer0/o Activity in NEBuffersHeatIna
8、ctivation Incuba.Temp.Diluent1234Aatll皿NEBuffer 4050501006537AAcc65IgukjNEBuffer 3 + BSA1075100256537AAccI血|NEBuffer 45050101008037AAcilNEBuffer 32550100506537AAcllNEBuffer 4 + BSA10100100No37BAcul血1NEBuffer 4 + SAM50100501006537BAfelGJNEBuffer 42550251006537AAflllGHZNEBuffer 4 + BSA50100251006537AA
9、flUIamNEBuffer 3 + BSA2575100508037BAgelGumNEBuffer 11005010756537CAhdIGHZ!NEBuffer 4 + BSA257501006537AAlel皿NEBuffer 41025101006537AAlulEUNEBuffer 4100100751006537BAlwlGnuNEBuffer 450100101006537AAlwNIaNEBuffer 450100501006537AApalGHZlNEBuffer 4 + BSA255001006525AApaLINEBuffer 4 + BSA10010010100No3
10、7ABmtl血|NEBuffer 22510025506537BBpmlamNEBuffer 3 + BSA751001001006537BBpulOI曲iNEBuffer 31025100258037BBpuEIMJNEBuffer 4 + SAM50100101006537BBsaAINEBuffer 4100100100100No37CBsaBINEBuffer 450100751008060BBsaHIQJNEBuffer 4 + BSA501001001008037ABsal血|NEBuffer 475751001006550BBsaJIGJNEBuffer 410010010010
11、08060ABsaWINEBuffer 4 + BSA50100501008060ABsaXINEBuffer 47510010100No37BBseRINEBuffer 4100100751006537ABseYI血1NEBuffer 31050100506537BBsglaNEBuffer 4 + SAM5075501006537BBsiEINEBuffer 4 + BSA50100101008060ABsiHKAINEBuffer 4 + BSA50100100100No65BBsiWINEBuffer 3100100100258055ABslINEBuffer 310501007580
12、55ABsmAINEBuffer 4501001001008055BBsmBIGJNEBuffer 3751001001008055BBsmFINEBuffer 4 + BSA2550501008065ABsmINEBuffer 475100751008065ABsoBINEBuffer 21010010050No37ABspl286INEBuffer 4 + BSA2525251006537ABspCNINEBuffer 4 + BSA + SAM10075101008025ABspDINEBuffer 42575501006537ABspEINEBuffer 301010008037BBs
13、pHI血1NEBuffer 410100501006537ABspMI血1NEBuffer 3NRNR100NR6537BBspQINEBuffer 410501001008050BBsrBIaNEBuffer 4501001001008037ABsrDINEBuffer 2 + BSA5010050758065ABsrFINEBuffer 410100100100No37CBsrGINEBuffer 2 + BSA25100101008037ABsrlaNEBuffer 3050100108065BBssHIINEBuffer 3100100100 dd1008050ABssKI血1NEBu
14、ffer 3 + BSA050100508060ABssSI画NEBuffer 3 + BSA050100 dd108037BBstAPI血1NEBuffer 4 + BSA251001001008060ABstBINEBuffer 4755025100No65ABstEIINEBuffer 3 + BSA507510075No60ABstNINEBuffer 2 + BSA1010010075No60ABstUINEBuffer 410010050100No60ABstXINEBuffer 3050100256537BBstYI血|NEBuffer 250100751008060ABstZ1
15、7INEBuffer 4NRNR100100No37BBsu36I皿NEBuffer 3 + BSA02510008037ABtglEUNEBuffer 3 + BSA25501001008037ABtgZINEBuffer 4 + BSA102501008060ABtsCINEBuffer 45010050100No50BBtsINEBuffer 4 + BSA10050501008055AEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB33。inB4HeatInactiva.Incuba.Temp.DiluentCac8INEBuffer
16、 450751001006537BClalNEBuffer 4 + BSA1050501006537ACspCI宵NEBuffer 4 + SAM10100101006537ACviAII画NEBuffer 47525101006525BCviKI-1血INEBuffer 41050251008037ACviQINEBuffer 3 + BSA7510010075No25BEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentDdelEmNEBuffer 375100
17、100756537ADpnlNEBuffer 4100100751008037BDpnII血INEBuffer DpnIINRNR100 ddNR6537BDralNEBuffer 47575501006537ADrainNEBuffer 3 + BSA10075100256537BDrdINEBuffer 4 + BSA2550101006537AEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentEaelNEBuffer 4100100501008037AEag
18、INEBuffer 31025100106537CEagl-HFNEBuffer 42550101006537CEarl血iNEBuffer 4100100501006537AEcilNEBuffer 2 + BSA50100100506537AEcoNI口NEBuffer 4100100751006537AEcoO 1091NEBuffer 4 + BSA100100751006537AEcoP15IamNEBuffer 3 + BSA + ATP751001001006537AEcoRINEBuffer EcoRI dd1001001001006537CEcoRI-HFwEM】NEBuff
19、er 41010001006537CEcoRVNEBuffer 3 + BSA5075100508037AEcoRV-HFMNEBuffer 4251001001006537BEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentFatlGJNEBuffer 21010050506555AFaulamNEBuffer 410050101006555AFnu4HINEBuffer 41025251006537AFokINEBuffer 4100100751006537A
20、FselGUONEBuffer 4 + BSA1007501006537BFspINEBuffer 4107510100 dd6537CEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentHaell血INEBuffer 4 + BSA75100501008037AHaelllMJNEBuffer 450100251008037AHgalGJNEBuffer 110075501006537AHhalEmNEBuffer 4 + BSA751001001006537AH
21、indi血1NEBuffer 3 + BSA751001001006537BHindlllNEBuffer 25010010506537BHinflNEBuffer 475100751008037AHinPlINEBuffer 41001001001006537AHpal皿NEBuffer 4255010100No37AHpallNEBuffer 11005010506537AHphINEBuffer 4NR7501006537BHpyl66IINEBuffer 4100100501006537CHpyl88I血1NEBuffer 45025101006537AHpyl88III血1NEBuf
22、fer 4 + BSA100100101006537AHpy99IGJNEBuffer 4 + BSA101001006537AHpyAVNEBuffer 4 + BSA100100251006537BHpyCH4III血|NEBuffer 410050251008037AHpyCH4IVNEBuffer 11002510256537AHpyCH4VGHQNEBuffer 45050251006537BEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentI-Ceul
23、QUNEBuffer 4 + BSA101001006537BI-ScelGJNEBuffer I-Scel + BSA105050506537BEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3“)inB4HeatInactiva.Incuba.Temp.DiluentKasIamNEBuffer 4 + BSA2510001006537BKpnlGumNEBuffer 1 + BSA10075050No37AEnzyme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatIna
24、ctiva.Incuba.Temp.DiluentMbolNEBuffer 4751001001006537AMboIINEBuffer 4100100501006537CMfel里NEBuffer 47550101006537AMfel-HFNEBuffer 47550101006537AMluINEBuffer 32575100506537AMlylNEBuffer 4 + BSA5050251006537AMmel Rli NEBuffer 4 + SAMNRNRNR100 dd8037BMnIINEBuffer 4 + BSA75100501006537BMsd血iNEBuffer 4
25、7575751006537BMselGJNEBuffer 4 + BSA75100751006537AMslIGHZNEBuffer 4100100251006537AMspAlIgukjNEBuffer 4 + BSA50100501006537BMspINEBuffer 475100501008037AMwol血1NEBuffer 3107510075No60BEnzyme topSupplied NEBuffer0/o inBlQ/o inB20/o inB33。inB4HeatInactiva.Incuba.Temp.DiluentNael血1NEBuffer 410075101006
26、537ANari曲1NEBuffer 410075751006537ANb.BbvCI皿NEBuffer 250100101008037ANb.BsmI血1NEBuffer 325100100258065ANb.BsrDI血1NEBuffer 21010025508065ANb.BtsI血1NEBuffer 4 + BSA75100751008037ANeilNEBuffer 41002510100No37ANcolNEBuffer 31001001001006537ANcoI-HFNEBuffer 450100101008037BNdelNEBuffer 475100751006537ANg
27、oMIVamNEBuffer 410050101008037ANhelGumNEBuffer 2 + BSA100100101006537CNhel-HFNEBuffer 4 + BSA1001001008037CjJNlalll皿NEBuffer 4 + BSA2525251006537BNlalVGJNEBuffer 4 + BSA1010101006537BNmeAIIIghqNEBuffer 4 + SAM101001008037BNotlNEBuffer 3 + BSA050100256537CNotI-HFMNEBuffer 4 + BSA25100251006537ANrulGH
28、KZ1NEBuffer 3010100106537ANsilNEBuffer 31075100258037ANspI皿NEBuffer 2 + BSA10010001006537ANt.AlwI血INEBuffer 22510025508037ANt.BbvCI血INEBuffer 450100101008037ANt.BsmAIGJNEBuffer 410050101006537ANt.BspQIMJNEBuffer 31075100258050BNt.BstNBI血INEBuffer 3010100108055ANt.CviPIIGJNEBuffer 425100501006537AEnz
29、yme topSupplied NEBuffer0/o inBlQ/o inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentPadMJNEBuffer 1 + BSA10075101006537APaeR7INEBuffer 42510010100No37APcil血iNEBuffer 35075100508037APflFINEBuffer 4 + BSA025251006537APflMINEBuffer 3 + BSA0100100506537APhol圄1NEBuffer 3505010075No75API-PspIamNEBuffe
30、r PI-PspI + BSA0101010No65BPl-Scel血iNEBuffer Pl-Scel + BSA00006537BPiel血iNEBuffer 4100100751006537APmelNEBuffer 4 + BSA050101006537APmllNEBuffer 1 + BSA100750756537APpuMINEBuffer 40250100No37BPshAI血|NEBuffer 4 + BSA5075101006537APsil血|NEBuffer 410100101006537BPspGIGJNEBuffer 47510050100No75APspOMIamNEBuffer 42525101006537APspXIamNEBuffer 40100101008037BPsUNEBuffer 3 + BSA7575100508037CRliPvulNEBuffer 3 + BSA1075100108037BRli,1PvuIINEBuffer 2100100100100No37BRli/PvuII-HFMNEBuffer 402501008037BEnzyme topSupplied NEBuffer% inBl% inB2Q/o inB3Q/o inB4HeatInactiva.Incuba.Temp.DiluentRs