《(精编资料推荐)常用酶切位点表(含保护碱基).docx》由会员分享，可在线阅读，更多相关《(精编资料推荐)常用酶切位点表(含保护碱基).docx（4页珍藏版）》请在三一文库上搜索。
1、切割率 %酶寡核苷酸序列2 hr20 hrAcc IG GTCGAC C00CG GTCGAC CG00CCG GTCGAC CGG00Afl IIIC ACATGTG00CC ACATGTGG>90>90CCC ACATGT GGG>90>90Asc IGGCGCGCC>90>90AGGCGCGCCT>90>90TT GGCGCGCCAA>90>90Ava IC CCCGGG G50>90CC CCCGGG GG>90>90TCC CCCGGG GGA>90>90BamH IC GGATCC G1025
2、CG GGATCC CG>90>90CGC GGATCC GCG>90>90Bgl IIC AGATCTG00GA AGATCTTC75>90GGA AGATCTTCC25>90BssH IIG GCGCGC C00AG GCGCGC CT00TTG GCGCGC CAA50>90BstE IIG GGT(A/T)ACCC010BstX IAACTGCAGAACCAATGCATTGG00AAAACTGCAGCCAATGCATTGG AA2550CTGCAGAA CCAATGCATTGG ATGCAT25>90Cla IC ATCGAT G00GA
3、TCGATC00CC ATCGATGG>90>90CCC ATCGAT GGG5050EcoR IG GAATTCC>90>90CG GAATTCCG>90>90CCG GAATTCCGG>90>90Hae IIIGG GGCC CC>90>90AGC GGCC GCT>90>901TTGC GGCC GCAA>90>90Hind IIIC AAGCTTG00CC AAGCTT GG00CCC AAGCTT GGG1075Kpn IG GGTACC C00GG GGTACC CC>90>90CGG
4、GGTACC CCG>90>90Mlu IG ACGCGT C00CG ACGCGT CG2550Nco IC CCATGG G00CATG CCATGG CATG5075Nde IC CATATGG00CC CATATG GG00CGC CATATGGCG00GGGTTT CATATG AAACCC00GGAATTCCATATGGAATTCC75>90GGGAATTCCATATG GAATTCCC75>90Nhe IG GCTAGC C00CG GCTAGC CG1025CTA GCTAGC TAG1050切割率 %酶寡核苷酸序列2 hr20 hrNot ITT GC
5、GGCCGCAA00ATTT GCGGCCGCTTTA1010AAATAT GCGGCCGCTATAAA1010ATAAGAAT GCGGCCGCTAAACTAT2590AAGGAAAAAA GCGGCCGCAAAAGGAAAA25>90Nsi ITGC ATGCAT GCA10>90CCA ATGCAT TGGTTCTGCAGTT>90>90Pac ITTAATTAA00G TTAATTAA C025CC TTAATTAAGG0>90Pme IGTTTAAAC00G GTTTAAACC025GG GTTTAAACCC0502AGCTTT GTTTAAAC GGC
6、GCGCCGG75>90Pst IG CTGCAG C00TGCA CTGCAG TGCA1010AA CTGCAG AACCAATGCATTGG>90>90AAAA CTGCAG CCAATGCATTGGAA>90>90CTGCAG AACCAATGCATTGGATGCAT00Pvu IC CGATCG G00AT CGATCG AT1025TCG CGATCG CGA010Sac IC GAGCTC G1010Sac IIGCCGCGG C00TCC CCGCGG GGA50>90Sal IGTCGAC GTCAAAAGGCCATAGCGGCCGC00G
7、C GTCGAC GTCTTGGCCATAGCGGCCGCG1050G1075ACGC GTCGAC GTCGGCCATAGCGGCCGCGGAASca IGAGTACT C1025AAA AGTACT TTT7575Sma ICCCGGG010CCCCGGG G010CC CCCGGG GG1050TCC CCCGGG GGA>90>90Spe IGACTAGT C10>90GG ACTAGT CC10>90CGG ACTAGT CCG050CTAG ACTAGT CTAG050Sph IG GCATGC C00CAT GCATGC ATG025ACAT GCATGC
8、 ATGT1050Stu IAAGGCCT T>90>90GAAGGCCT TC>90>90AAA AGGCCT TTT>90>903Xba ICTCTAGAG00GC TCTAGAGC>90>90TGC TCTAGAGCA75>90CTAG TCTAGACTAG75>90Xho IC CTCGAG G00CC CTCGAG GG1025CCG CTCGAG CGG1075Xma ICCCCGGG G00CC CCCGGG GG2575CCC CCCGGG GGG50>90TCCC CCCGGG GGGA>90>904