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1、西南程序设置 本程序只能在Office2003上运行(建议完全安装) 设置Word宏安全:工具-宏-安全性-低 关闭当前Word文档 再打开本文件大学 任课部门选择 任课教师所在部门 按拼音顺序排列 分两个选择框显示 在后框中选择时,前框中选空白项,反之既然. Molecular Biology 课程 20072008学年 第学期期 考试时间120分钟考核方式学生类别人数98适用专业或科类生物技术年级05 级题号合计得分签名阅卷须知：阅卷用红色墨水笔书写，得分用阿拉伯数字写在每小题题号前，用正分表示，不得分则在题号前写0；大题得分登录在对应的分数框内；统一命题的课程应集体阅卷，流水作业；阅卷后
2、要进行复核，发现漏评、漏记或总分统计错误应及时更正；对评定分数或统分记录进行修改时，修改人必须签名。特别提醒：学生必须遵守课程考核纪律，违规者将受到严肃处理。 命题须知 命题内容须以教学大纲规定的知识内容、能力培养以及相关的教学目标层次要求为依据，课程内容覆盖率80%。 在一般的闭卷笔试中，有关论述题、综合题、辨析题等考查较高能力层次的题型的分值应占有较大比例。 试题题意应清晰明确，文字应准确简练。 A、B两套考题在知识单元、题型、题量、难度和分值上必须相当。 同一门课程本次考题与上次考题以及A、B两套考题的题目重复率应10%。 每套考题一般应有四个以上题型。每大题题干后应注明小题数、分值和大
3、题总分。试题下面应留出答题空间。 试题题量一般应以在110分钟考试时间内做完为标准。 每套试题应有相应的参考答案与评分标准。I Define the meaning of these following terms (30 points, 3 pts per term)1. melting temperature (Tm)2. C value paradox3. exon4. semi-conservation replication5. cDNA library6. gel shift assay7. promoter8. open reading frame (ORF)9. gene fa
4、mily10. expressed sequence tag (EST)II Fill in blank (10 points, 1 pts per blank)1. The simplest cloning vector at least contains , and ori elements.2. The diagram below shows two RNA molecules. Molecule I is processed to give molecule II. Answer the following questions about these two molecules:Whe
5、re does this conversion happen? _What type of RNA molecule is molecule I? _What type of RNA molecule is molecule II? _Name the regions/structures indicated by the arrows:A = _ B = _ C = _ D = _ E = _ III Select the right answer (20 points, 2 pts per question; Select the best answer for each question
6、.)1. The bond between a phosphate group and the ribose sugar groups in RNA is called which of the following? ( )(A) Phosphodiester bond (B) Glycosidic bond (C) Peptide bond (D) Amide bond 2. Which enzyme does not play a role in DNA replication? ( )(A) DNA polymerase (B) Helicase (C) Single-stranded
7、binding protein (D) Guanyl transferase 3. Splicing is the process that does which of the following? ( )(A) Remove introns and conserve exons (B) Remove exons and conserve introns (C) Remove mutated regions of primary transcript RNA (D) Add multiple adenosine bases to the end of a primary RNA transcr
8、ipt 4. The ideal spacing between the -10 and -35 regions of a prokaryotic promoter is: ( ) bp.(A) 14(B) 15 (C) 16 (D) 17 5. The 5 capping process creates what type of linkage? ( )(A) 5-3 (B) 3-5 (C) 5-5 (D) 3-3 6. Codons that code for the same amino acid are called: ( )(A) Synonyms (B) Similar (C) D
9、egenerates (D) Complements 7. Which of the following is not a characteristic of a tRNA molecule? ( )(A) D arm (B) Beta arm (C) T arm (D) Anti-codon arm8. What signals the end of transcription? ( )(A) Stop codon (B) Terminator (C) The end of the DNA chain (D) RNA polymerase runs out 9. The start codo
10、n is: ( )(A) AUU (B) UAA (C) AUG (D) UAG 10. Which of the following is the sequence of the -10 region of prokaryotic promoters? ( )(A) TATAAT (B) TTGACA (C) TATA (D) CAA IV Analytical questions (30 points, 6 pts per question)1. Describe briefly the attenuation of the trp operon.2. Describe the princ
11、iple of Sangers enzymic method for DNA sequencing.3. What are the basic features of DNA double helix deduced by Watson and Crick?4. Please draw a schematic structure of TTP in the following space. You may write just the letter T for the base.5. On the following agarose gel with marker DNA as indicat
12、ed:a) Place a plus (+) sign on the positive pole of the gel and a minus () sign on the negative pole.b) Draw the band or bands that you would see if you digested a 3 kb plasmid with EcoRI and ran it to the left of the marker. Assume the plasmid has a MCS with the following restriction sites: BamHI, EcoRI, EcoRV, HindIII, and KpnI.V Discuss the following topic (10 points)Describe your understanding of Central Dogma in detail.