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1、Program of Study:General MedicineCourse:BiochemistryAbbreviation:LCH/VAA21Schedule:45 hours of lectures60 hours of exercisesCourse Distribution:2nd year, 3rd semesterNumber of Credits:0Course Form:Lectures, exercisesLectures :Teachers :Doc. RNDr. Daniela Walterov, CSc.Prof. RNDr. Jitka Ulrichov, CSc
2、.RNDr. Zdenk Dvok, PhD.Study :ContinuousDateSubjectNo. of Less.Teacher121.09.2006The scope of biochemistry, its role in medicine.Proteins structure, function.3Walterov228.09.2006State holiday-305.10.2006Heteroproteins.Structure and function of hemoglobin. 2Walterov412.10.2006Enzymes-structure, class
3、ification, mechanism of action. Cofactors.3Dvok519.10.2006Enzyme activity. Regulation of the enzyme activity. Enzymes in diagnosis.2Walterov626.10.2006Nucleotides structure, function.Metabolism overview. 2Walterov702.11.2006Bioenergetics. High-energy compounds structure, biological role.Biological o
4、xidation overview. 2Ulrichov809.11.2006 Respiratory chain, oxidative phosphorylation.Citric acid cycle. AcetylCoA-central role in metabolism. 3Ulrichov916.11.2006Carbohydrate metabolism over-view. Digestion of carbohydrates.2Dvok1023.11.2006Glycolysis.Gluconeogenesis.2Dvok1130.11.2006Glycogen metabo
5、lism.Pentose phosphate pathway.2Dvok1207.12.2006Lipid metabolism overview.Digestion of lipids.2Walterov1314.12.2005Lipoproteins structure, metabolism, diagnostic significance.2Walterov1421.12.2006Triacylglycerol and phospholipid metabolism.2 Walterov1504.01.2007Interrelations in carbohydrate and lip
6、id metabolism. 2WalterovPractical training:Leading Teacher :RNDr. Zdenk Dvok, Ph.D.Study :ContinuousWeek from-toSubjectNo. of Less.118.9. - 22.9.2006Rules of study and directions for laboratory work. System for blood collection. Blood. Plasma. Serum. 4 (Vi)225.9. - 29.9.2006Chemical reactions of ami
7、no acids and proteins.Separation of acidic, neutral and basic amino acids by electrophoresis.Determination of isoelectric points of histidine. 4 (Va)32.10. - 6.10.2006 Thin layer chromatography of amino acids. Isolation of albumins and globulins by fraction salting out.4 (Va)49.10. - 13.10.2006Deter
8、mination of proteins (Biuret method).Electrophoretic separation of proteins.Determination of isoelectric point of casein.4 (Va)516.10. - 20.10.2006Enzymes introduction: nomenclature, structure, factors influencing enzyme activity.4 (Vi)623.10. - 27.10.2006Demonstration of enzymatic character of pero
9、xidase reaction.Demonstration of substrate specificity of a-amylase and sucrase.Inhibition of enzymatic activity - inhibition ofcatalase by cyanide.Determination of pH optimum for trypsin and pepsin.4 (ZD)730.10. - 3.11.2006Chemical reactions of saccharides.Polarimetric observations of D-glucose mut
10、arotation. Nucleoproteins isolation.4 (Dv)86.11. - 10.11.2006Chemical reactions of lipids.Determination of the iodine number of lipids.4(Va)913.11. - 17.11.2006Determination of acetylsalicylic acid in Acylpyrin.4 (ZD)1020.11. - 24.11.2006Deionization of calcium chloride solution on ion exchangers.Ge
11、l chromatography of hemolyzed blood.4 (Dv)1127.11. - 1.12.2006Quantitative determination of salicylates by photometry.Determination of vitamin C in fruit juices.4 (ZD)124.12. - 8.12.2006Isolation and demonstration of alkaloids fromChelidonium majus.4 (ZD)1311.12. - 15.12.2006Identification of levome
12、promazine and its metabolites in biological materials by thin layer chromatography.Preparative chromatography of azo dyes on a thin layer.Determination of nitrates.4 (ZD)1418.12. - 22.12.2006Practical photometric test. 4 (Dv)151.1. - 5.1.2006Chemical properties of substances of biological importance an overview. Course unit test.4 (Dv)Completed by :Course unit creditRequirements :100% presence in practicalsLiterature :1. P. C. Champe, R. A. Harvey: Biochemistry (2nd or 3rd edd.), Lipincott Comp. 1994,2. L. Stryer: Biochemistry (3rd or 4th edd.), Freeman, New York1988.