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1、常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液(0.05mol/L )X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀释至200毫升pHXYpHXY2.050一44.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07 , 0.2 mol/L甘氨酸溶液含15.01克/升2.邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L 邻苯二甲酸氢钾+ 0.2 mol/L HCl，再加水稀释到20毫pH(20C)XYpH(20 C)XY2.25P 4.0703.25r
2、 1.470 2.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量 =204.23 , 0.2 mol/L邻苯二甲酸氢溶液含 40.853.磷酸氢二钠-柠檬酸缓冲液pH0.2mol/LNaHPO(毫升)0.1mol/L柠檬酸(毫升)pH0.2mol/L NaHPO (毫升)0.1mol/L柠檬酸(毫升)2.20.4010.605.210.72F 9.28 12.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913
3、.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55NaHP龄子量=14.98 , 0.2 mol/L 溶液为 28.40 克
4、/ 升。NaHPQ 24O分子量=178.05 , 0.2 mol/L 溶液含 35.01 克/ 升。CHQ RO分子量=210.14 , 0.1 mol/L 溶液为 21.01 克/升。4 .柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L ）柠檬酸（克）GHO H.O氢氧化钠（克）NaOH 97%盐酸（星升）HCl （浓）最终体积（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用时可以
5、每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5.柠檬酸-柠檬酸钠缓冲液（0.1 mol/L ）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.0
6、4.610.39.76.61.418.64.89.210.8柠檬酸GHO H2O:分子量210.14 , 0.1 mol/L 溶液为21.01克/升。柠檬酸钠Na C6H5O 2HQ 分子量294.12 , 0.1 mol/L 溶液为29.41克/毫6 .乙酸-乙酸钠缓冲液（0.2 mol/L ）pH（18C）0.2 mol/LNaAc（毫升）0.3 mol/L HAc （毫升）pH（18 C）0.2 mol/L NaAc（毫升）0.3 mol/L HAc（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.10
7、4.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc 3HO分子量=136.09 , 0.2 mol/L 溶液为 27.22 克/ 升。7.磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）pH0.2 mol/LNa2HPO（毫升）0.3 mol/LNaH2PO（毫升）pH0.2 mol/L NaHPO （毫升）0.3 mol/LNaJPO（毫升）5.88.092.07.0 161.039.0 15.910.090.07.167.033.06.012.387.77.272.028.06.115.085
8、.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0NaHPO 2HO分子量=178.05 , 0.2 mol/L 溶液为 85.61 克/ 升。NaHPO 12H2O分子量=358.22 , 0.2 mol/L 溶液为 71.64 克/ 升。NaHPO 2H2O分子量=156.03 , 0.2 mo
9、l/L 溶液为 31.21 克/ 升。（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15 mol/L ）pHmol/L/15Na2HPO4（毫升）mol/L/15KH 2PpHmol/L/15Na 2HPmol/L/15KH 2PQ（毫升）Q（毫升）Q（毫升）4.920.109.907.17.003.005.290.509.5078.002.005.911.009.007.39.001.006.242.008.0089.500.506.473.007.007.79.750.256.644.006.0039.900.106.816.985.006.005.004.008.048.348.678.181
10、0.000NaHPQ 2HO分子量=178.05 , 1/15M 溶液为 11.876 克/ 升。KHPO分子量=136.09 , 1/15M溶液为9.078克/升。8.磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO + Y毫升0.2N NaOH加水稀释至29毫升pH(20C)X （毫升）Y （毫升）pH(20 C)X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液
11、（18C）pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M巴比妥钠溶液（毫升）0.2N盐酸（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10. Tris -盐酸缓冲液（0.05M, 25C）50毫升0.1M三羟甲基氨基甲烷（Tris ）溶液与X毫升0.1N盐酸混匀
12、后，加水稀释至100毫升pHX（毫升）pHX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris ） HOCH2 CH2OHHOCH2 NH2分子量=121.14;0. 1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将 瓶盖严011 .硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂
13、（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBO吒0,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸FbBQ,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11.硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58
14、.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaB07 10RO,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸F2B0,分子量=61.84, 0.2M 溶液为12.37克/升。硼砂 易失去结晶水，必须在带塞的瓶中保存。12.甘氨酸-氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOHtt水稀释至200毫升pHXYpHXY8.650r 4.09.650P 22.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.8
15、10.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升13 .硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOFto水稀释至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂NaBO 10HO，分子量=381.43;0.05M溶液为19.07克/升。14.碳酸钠-碳酸氢钠缓冲液（0.1M） CaT、Mg+存在时不得使用pH0.1MNaCO（毫升）0.1MNHCO（毫升）20 C37 C9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791NaCO 10H2O分子量=286.2;0.1M 溶液为 28.62 克/ 升。MHC龄子量=84.0;0.1M 溶液为8.40克/升。15. “PBS缓冲液PH7.67.47.27.0H2O100010001000100ml nNaCl8.58.58.58.5gNaHPO2.22.22.22.2gNaHPO0.10.20.30.4g