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1、中南大学学报(医学版Cent South Univ (Med Sci)2012； 37(8) http:/wwwxsumed.org；790慢性轻度不可预见性应激抑郁模型大鼠血清同型半胱氨酸和抗环瓜氨酸肽抗体浓度升高唐亚梅-刘勇S秦立新张仁生S陈若虹'(中南大学湘雅二医院1 检验科；2梢神卫生研究所长沙410011)摘要目的：观察慢性轻度不可预见性应激(chronic unpredictable mild stress. CUMS)诱导的抑郁模型大根血 清同型半胱氨酸(homocysteine, Hey)和抗环瓜氨酸肽(anti-citrullinatcd peptide, anti-
2、CCP)抗体浓度的变化，探讨抑 郁症的免疫机制及其与门身免疫的关系。方法:60只成年雄性SD大国随机分为2大组，每组30只，每大组再分 为正常对照组、模型组和氟西汀(fluoxetine. Fix)治疗组3个亚组。以CUMS结合孤养建立抑郁人鼠模型，造模完 成后，第一大组大鼠用于旷场试验、糖水消耗试验和强迫游泳试验；第二大组大鼠心脏穿刺采血后，以循环礴法 测定血清Hey水平，ELISA法测定血清anti-CCP抗体浓度。结果：与对照组和氟西汀组比较，模型组大鼠自发活 动显著减少；糖水消耗戢、糖水偏爱百分比显著降低；强迫游泳不动时间明显延长；血清Hey和anti-CCP抗体水 平明显升高。结论：
3、CUMS抑郁模型人鼠体内存在免疫炎症和身免疫反应，氟西汀治疗町改善上述免疫反应。关键词抑郁症；CUMS；免疫；同型半胱氨酸；anti-CCP抗体DOI:10.3969/j.issn.l672-7347.2012.0&006Concentration increase in Hey and anti-CCP antibody inthe serum of depression rat model induced bychronic unpredictable mild stressTANG Yamci1, LIU Yong2, QIN Lixin1, ZHANG Rcnsheng1, C
4、1IEN Ruohong1(1. Department of Clinical Laboratory； 2. bistitute of Mental Health, Sea)nd Xiangya Hospital, Central South University, Changsha 410011, China)ABSTRACTObjective： To observe the change of serum homocysteine (Hey) and anti-citrullinated peptide (CCP) antibody concentration in depression
5、rat model induced by chronic unpredictable mild stress (CUMS), and to explore the immunologic mechanism of depression and the relation between depression and its autoimmunity.Methods: Sixty adult male SD rats were randomly divided into 2 groups, 30 rats in each group, which were divided into 3 subgr
6、oups： a normal control group, a model group and a fluoxetine- treated group. The depression rat model was established under CUMS and seperated feeding, after which, open field, sugar consumption and forced swimming test were applied in the first group. After the blood was taken in the second group of rats through heart puncture, the level of serum收稿日期(Date of reception): 2012-04-09作者简介(Biography)：唐亚梅，硕士.主管技师.主要从鄧临床免疫研究。通信作老(Corresponding author):庸亚梅.Email: tayamel39基金项目(Foundation item):国家白然科学基金(81171268) This work was wuppnrlrd by the National Natural Science Fourxlatinn of China (81171268).