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1、pGADT7 AD载体信息ADHlAm p1pGADT7 ADHjndUI(1479)SV40 NLSGAL4 ADHihdru12273MCS1414202SpUConMtrbniEiljBr S AD L3 Insert Sc me ring AjnptimeirLEU2卩301輛代i叫mm初I? PronMXtnCTA no GAr GAT GAA G Ar ACC CCA CCA AAC CCA AM AAA GA3 ATC ITT Ml ACG ACTSTAPT#1)fktHACAL： 1 AT AGG GCG AjCGCCGCCATG GAl TAL 13 TAL GAC GTAC
2、CAGAT TAG GCCAT ATG GCC AFG GAG GCC AGT GAATTC CAC CCG GGT GGG CAT CCA TAG GGG ATC CATCt；A GC1 CGA GCI GCAGAItA.flTCGTAIACTGAAAAACCCCKCAATrCACnCMichntktrj adj nn scronq5 ClaLRumhHAACTGTGCATCGTGCACCATCT3 AD SequOKinj Prm*pGADT? AD Vector Map and Multiple Cloiin Site (MCG)DescriptionpGADT7 AD is a yea
3、st expression vector that is designed to express a protein of interest fused to a GAL4 activation domain (AD; amino acids 768-E81 J.Transcription of the GAL4 AD fusion is driven by the constitutively active ADH1 promoterard is terminated at theADH1 廿 an scription termination signal (乙。屮).The GAL4 AD
4、 fusion contains an N-termi nal SV40 nuclear localization signal (SV40 NLS; 1) that targets the protein to the yeast nucleus, and a hemagglutinin epitope tag (HATagh located between the GAL4 AD and the protein of interest, that allows the protein to be easily detected with HA-tag antibodies.TheT7 pr
5、omoter (Pr?h located just upstream of the HA tag sequence, allows in v/tro transcription and translation of the HA-tagged protein of interest ,CG1945(MA7h HF7c(/W4Ta) Selectable marker: LEU2 S cerevisiae origin: 2 pReference1. Chienr C匸 Bartd. R L. Sternglaru. R. &S. 11991) Proc. Nati. Acd. Sa. USA 88:9678-9S82.Note: The vector sequence was compiled from information in the sequence databases, published literature, and oxher sources, xogexher witn panial sequences obtained by Cion(ocn.This veexor has nor boon compiexeiy seque need.