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1、 CtBP1论文：转录辅助抑制因子C-末端结合蛋白在食管癌中的表达【中文摘要】检测CtBP1和p16在食管癌组织中的表达,探讨其在食管癌发生、发展中的作用及相关性。方法：采用免疫组化的方法检测CtBP1和p16在57例食管癌患者癌组织中的表达,分析其临床意义。其中57例中鳞癌46例,腺癌11例。期11例,期23例,期23例,期0例(根据国际抗癌联盟(UICC)的标准进行TNM分期)。其中淋巴结阳性转移41例,无淋巴结阳性转移16例。另取30例距肿瘤组织4-5cm的癌旁组织作为对照。结果：57例食管癌病例标本中,CtBP1和p16蛋白表达阳性率分别是90%(51/57)和52%(30/57)。C
2、tBPl蛋白在鳞癌和腺癌中的阳性率分别为85.9%、90.9%,两者无统计学差异(p=0.682),表明CtBP1的表达与病理类型无相关性。期、期、期的阳性率分别为90.9%、82.6%、82.6%,三者无统计学差异(p=0.334),有淋巴结转移和无淋巴结转移的组织中的阳性率分别为88.3%、87.5%,两者无统计学差异(p=0.675),30例癌旁组织中的阳性率为96.7%,与癌组织相比差异无统计学意义(p=0.667),表明CtBP1的表达与食管癌的分期无相关性。p16蛋白在鳞癌和腺癌组织中的表达阳性率分别为49%和44%,两者无统计学差异(p=0.687),p16蛋白在期、期、期组织中
3、的表达阳性率分别为65.6%、35.5%、22.3%,三者有统计学差异(p=0.008),p16蛋白在淋巴结转移和无淋巴结转移的组织中的表达阳性率分别为32.1%、59%,两者有统计学差异(p=0.023),在对照的癌旁组织中的阳性率为80%,与癌组织相比差异有统计学意义(p=0.003),表明肿瘤分期越早,p16表达的阳性率越高。CtBP1蛋白的表达与p16蛋白的表达呈负相关(r=-0.381,p=0.001)。结论：CtBP1表达可能与食管癌的发生及发展相关,p16的表达缺失与食管癌的发生、发展密切相关,可能成为食管癌诊治的新靶标。【英文摘要】:This study was designe
4、d to detect the expression of CtBP1 in esophageal cancer.Methods:Immunohistochemical staining was used to detect the expression of CtBP1 in 57 esophageal cancer tissues. Of 57 cases of esophageal cancer,there were 46 cases of squamous carcinomacases of adenocarcinoma,cases ofstage,23cases ofstage,23
5、 cases ofstage,41 cases of lymph node metastasis and 16 cases of no lymph node metastasis. And there were 30 cases of corresponding non-tumor normal tissues for comparison.Results:Of 57 cases of esophageal cancer,90%(51/57) cases showed positive for CtBP1.The positive expressions for CtBP1 protein o
6、f the cases of squamous carcinoma and adenocarcinoma were 85.9% and 90.9%, without statistical significance (p=0.682). The positive expressions for CtBP1 protein of the cases ofstage,stage andstage were 90.9%、82.6% and 82.6%, without statistical significance (p=0.334). The positive expressions for C
7、tBP1 protein of the cases of lymph node metastasis and no lymph node metastasis were88.3%、87.5%, with no statistical significance (p=0.675). In the 30 cases of corresponding non-tumor normal tissues, the positive expressions for CtBP1 protein was 96.7%, with no statistical significance (p=0.667). Th
8、e positive expressions for p16 protein in the tissues of squamous carcinoma and adenocarcinoma were 49%、44%, respectively.And there was no statistical significance (p=0.687). The positive expressions for p16 protein in the tissues ofstage,stage andstage were 65.5%、36.6% and 22.3%, respectively. And
9、there was statistical significance (p=0.008). The positive expressions for p16 protein in the tissues of lymph node metastasis and no lymph node metastasis were 32.1% and 59% respectively. And there was statistical significance (p=0.023). In the 30 cases of corresponding non-tumor normal tissues, th
10、e positive expressions for p16 protein was 80%, with statistical significance (p=0.003). The expression of CtBP1 and p16 had negative correlation(P0.05, r=-0.381).Conclusions:The expression of p16 protein was associated with the carcinogenesis and development of esophageal cancer. CtBP1 can serve as
11、 the repressor of p16 and the potential target for the treatment and prognosis of esophageal cancer.【关键词】CtBP1 食管癌 免疫组织化学【英文关键词】CtBP1 ESOPHAGEAL CANCER IMMUNOHISTOCHEMICAL STAINING【备注】索购全文在线加好友：1.3.9.9.3.8848 同时提供论文写作一对一指导和论文发表委托服务【目录】转录辅助抑制因子C-末端结合蛋白1（C-terminal binding proteinl，CtBP1）和p16在食管癌中的表达摘
12、要2-3Abstract3引言6-7第1章 实验材料与方法7-91.1 研究对象71.2 主要实验仪器及耗材71.3 免疫组化研究方法7-81.4 统计学处理8-9第2章 结果9-112.1 CtBP1蛋白在食管癌、正常组织中的表达92.2 p16蛋白在食管癌中的表达9-102.3 CTBP1蛋自和p16蛋白在食管癌中表达的相关性10-11第3章 讨论11-133.1 CtBP1生物学功能及其与肿瘤发生、发展的关系113.2 p16在食管癌表达与意义11-123.3 CtBP1在食管癌表达及其与p16表达的相关性12-13结论13-14参考文献14-16文献综述16-32参考文献26-32攻读学位期间的研究成果32-33附图33-36致谢36-37