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1、CAPTER 5 COMPLEMENT （补体）,DEPARTMENT OF IMMUNOLOGY SICHUAN UNIVERSITY E-MAIL:,DEFINITION,Bacteria + Antibody + normal serum Soluble（细菌溶解） Bacteria +antibody + 55 prepared serum（55） Insoluble（细菌不溶解） What you learn from this experiment,?,What is complement,?,A group of heat-labile normal proteins which
2、 distribute in serum,humour and some cell membrane.They closely relate to the immune function and have enzymatic activation after actived（分布于血清，体液和一些细胞膜表面的一组热不稳定正常蛋白。与免疫功能紧密相关，活化后具有酶活性） What is the property of the complement （补体有和特点）,?,1. heat-labile （热不稳定） 2. normal proteins ,is independent of anti
3、gen stimulation（正常蛋白，与抗原刺激无关） 3.not a singly,there are about 30 kind of protein consist of a group,called complement system（不是一个单一成分，大约30种蛋白组成补体系统） 4.have enzymatic activation（具有酶活性） 5.play an important rloe in immune function（在免疫功能中发挥重要作用）,The consist of complement system （补体系统的组成）,1.the immanent c
4、ompose（固有成分） C1C9,D,B,P factor,MBL,serine protease（苏氨酸蛋白酶） 2.the regulative protein（调节蛋白） 3.the complement receptors（补体受体）,THE PATHWAY OF COMPLEMENT ACTIVATED （补体活化途径）,THREE DIFFERENT PATHWAYS（三条途径） 1. Classical pathway（经典途径） 2.Alternative pathway（替代途径） 3.Lectin pathway（凝集素途径，MBL途径）,CLASSICAL PATHWA
5、Y,commonly initiated by the formation of soluble antigen-antibody complexes or by the binding of antibody to antigen on a suitable target （有可溶性抗原-抗体复合物或者抗体结合到相应靶细胞上启动）,The condition of Complement activation via the classical pathway （经典途径活化补体的条件）,1. The formation of antigen-antibody complexes （抗原-抗体
6、复合物的形成） 2.only C1 bind with CH3 of IgM or CH2 of IgG1,IgG2,IgG3（C1与IgM的CH3或IgG1,IgG2,IgG3的CH2功能区结合） 3.one C1 must bind with at least two CH3 or CH2（C1必须与两个CH3或CH2结合）,The actived process （活化过程）,C1 recognize antigen-antibody complex （C1对抗原-抗体复合物的识别）,The structure of C1 and its recognition （C1的结构和识别）,T
7、he activation of complemant （补体的活化）,Ag-Ab,C1,C1,C1,C1,C4,C4a C4b,C2,C2b C2a,C4b2b,C4b2b3b,C3,C3b C3a,C5,C5a C5b,C6,C7,C567,C8,C5678,nC9,C5678(9)n,The Lysis. of Target（靶细胞的溶解）,C4,Classical Pathway Generation of C3-convertase,Classical Pathway Generation of C3-convertase,C4b,Mg+,C4a,C2,C4b2b is C3 con
8、vertase,Classical Pathway Generation of C5-convertase,C4b,Mg+,C4a,C2a,C3,C4b2a3b is C5 convertase; it leads into the Membrane Attack Pathway,Lytic pathway C5-activation,C5,Lytic pathway assembly of the lytic complex,C6,Lytic pathway: insertion of lytic complex into cell membrane,C 9,C 9,C 9,C 9,C 9,
9、C 9,C 9,C 9,C 9,ALTERNATIVE PATHWAY （替代途径）,The condition of Complement activation via the alternative pathway （替代途径活化的条件）,Bacteria,LPS,poly IgA or IgG4 （细菌，LPS，IgA多聚体或IgG4),激活物质 细菌内毒素、酵母多糖、葡聚糖、凝集的IgA和IgG4等 B C3 C3b C3bB C3bBb C3a C3a Ba C3b D C3 C3bnBb C5a C5 C5b nC9 C8 C6,C7 C5678 C567 C56789(n),LE
10、CTIN PATHWAY (MBL途径）,The condition of Complement activation via the lectin pathway （补体MBL途径活化的条件）,Bacteria,MBL,serine protease （细菌，MBL，苏氨酸蛋白酶）,The structure of MBL,MBL,+,病原体甘露糖残基,+丝氨酸蛋白酶,C4,C2,MASP,C4a + C4b,C2a + C2b,+,C4b2b C3转化酶,The differents between the three pathways （三条途径的区别）,Regulation of th
11、e activation of complement （补体活化的调节）,1.atuo-disintegration of complement（自身衰变的调节）,2. The regulation of Serum-soluble proteins（血清可溶性蛋白的调节）,1) C1 inhibtior (C1INH) （C1抑制因子）,2) C4 bingding protein,C4bp （C4结合蛋白）,3) factor I,4) factor H,5)protein S,Membrane-bound proteins （膜结合蛋白）,2)DAF(decay-accelerating factor，延缓加速因子）,3) HRF(homologous restriction factor，同源限制性因子),Lysis of foreign material（溶解细菌）,Opsonization（调理作用）,Solubilization and clearance of immune complexes（溶解和清除免疫复合物）,Inflammatory processes（介导炎症反应）,Neutralization（中和作用）,Immune regulation（免疫调节）,再见,