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5、3DE123 E3278F G3B65B0H =7B ?323 23I70J 38 0 67;767H3 053 3B3=027= I73B8 3II3=04 ?57=5 ?323 =;3=038 0 : 03EJ8?; 02:;I2632 :;8 : A:27:KB3 02:;I2632 ?705 : I7D38 =:B3 05:0 ?: EB1CC38 7;0 : (- GH4 *- L M+ 12=3F N53 =12J 23;0 IB?7;C 7;0 =7B ?: K32A38 K9 : 61B0763032FO53; 053 =7B ?323 3;32C7H384 053 (- GH
6、 6:C;307= I73B8 =:; K3 23C1B:038 I26 - 0 P- 6NF +;02B =1B0123 ?323 21; =;=1223;0B9 1;832 053 :63 =;8707; ?705 053 3DE38 =1B0123 K10 7; : 3E:2:03 7;=1K:02 ?57=5 ?: I233 I 6:C;307= I73B8 8127;C 053 QJ8:9 =1B0123 E2=3F R#M C2:873;0 3B3=02E5237 S0 ?: =:22738 10 0 8303=0 :EE07 :;8 R#M 83C2:8:07;4 :;8 : 6
7、87I738 63058 ?: 138 T10 : N:C: :; 9% 83=27K38 U7H:07; ; 8:9 QF+3BB ?323 ?:538 ?705 WX“ :;8 B938 :0 , Y I2 $- 67;103 7; B97 K1II32 Z$- 66B. N27JG+B EG QF-4$- 66B. RNM EG QF-4( C. N270; J$-FMI032 =3;027I1C:07; :0 $P - 2.67; :0 , Y I2 P- 67;1034 053 1E32;:0:;0 I =3BB B9:03 ?: 023:038 ?705 /_:3 M Z,- ;C
8、. :;8 E2037;:3 Z,- ;C. :0 P _:+B ?: :8838 0 -F( 6B. :;8 R_M ?: 23=A3238 K9 7E2E:;B E23=7E70:07; :0 *- Y A32;7C50FR_M ?: 87J BA38 7; $- ! I N K1II32FM ( ! R_M :6EB3 ?: 3B3=J 02E52338 ; : *- C. :C:23 C3B :;8 30578716 K26783ZX 0:7;7;C ?: 138 I2 A71:B7H:07;F G3=50 PP*(Q 0:7;7;C M 687I738 G3=50 PP*(Q 0:7
9、;7;C 63058 ?: 138 : : ;3=3J :29 :9 UQVF BB3=038 K9 029E7;7H:07;4 :;8 053; ?:538 ?705 WX“ :;8 I7D38 7; ,- C. E:2:I26:B835983 I2 *- 67;103 :0 26 036E32:0123F “1KJ 3b13;0B94053 =3BB ?323 ?:538 :;8 231E3;838 7; *- ! WX“ K3I23 83E707; ; =A32B7E :;8 ?323 B3I0 0 :85323 ; =A32 B7E I2 P- 67;103 :0 26 036E32:
10、0123FN53 =A32B7E ?323 023:038 ?705 G3=50 PP*(Q I2 P- 67;1034 27;38 ?705 WX“ 0?7=3F N53 =3BB ?323 A73?38 ?705 _7a; =B7E3 N*-J“ IB123=3;=3 67=2=E3F M=2787;3 2:;C3.30578716 K26783 ZM1KB3 0:7;7;C N57 63058 =6K7;38 053 87II323;07:B 1E0:a3 I IB123=3;0 R_M K7;87;C 893 M2E5BC7=:B :E3=0 I =52J 6:07; =;83;:07
11、; 7; 053 0:7;38 ;1=B314:BB?7;C ;3 0 8707;J C175 A7:KB3 =3BB ZL4 :EE007= =3BB 7; 3:2B9 0:C3 ZM4 :EE007= =3BB 7; B:03 0:C3 ZM :;8 ;3=207= =3BB Z_ U)VF M A7:KB3 =3BB ?1B8 5:A3 : 1;7I26 K27C50 C233; ;1=B31c :; 3:2B9 :EE007= =3BB4 ?53 636K2:;3 ?323 07BB 7;0:=0 K10 5:8 0:2038 0 =B3:A3 70 R_M4?1B8 5:A3 : C
12、233; 2 B7C50 93BB? ;1=B314K10 =526:07; =;83;:07; K3=:63 A77KB3 7; 053 I26 I K27C50 C233; E:0=53c : B:03 :EE007= =3BB ?1B8 5:A3 K27C50 2:;C3 :23: I =;83;38 =526:07; 7; 053 ;1=B31c:;8 : ;3=207= =3BB ?1B8 5:A3 :; 1;7J I26 K27C50 2:;C3 ;1=B31 U)4$-VF MEE007= =3BB ?323 3:7B9 8707;J C17538 I26 ;3=207= =3B
13、B K3=:13 053 B:0032 ?: 2:;C3 ?705 : ;26:B ;1=B3:2 021=0123 U$VF BB3=038 K9 029E7;7H:07;4 :;8 053; ?:538 ?705 WX“ 0?7=3F N53 =3BB ?323 23J 1E3;838 7; *- ! WX“4 :;8 053; 023:038 ?705 Q ! MJ B107;Z$- 6C. WX“ I 3:=5 893FN53 0:7;38 =3BB ?323 83J E7038 ; =A32B7E :;8 3D:67;38 17;C _7a; =B7E3 N *-J “ IB123=
14、3;=3 67=2=E9 :;8 E50C2:E538FMK10 *- =3BB I 3:=5 :6EB3 ?323 =1;038 ?705 053 IB123=3;=3 67=2=E9 I2 P 0763 U$4$*VF MEE007= 2:03 Zd e ZMfgM.ZLfMfgMf_ h $-d “0:0707=:B :;:B97i N53 8:0: ?323 :;:B9H38 K9 I720 :1052 ?705 8 053 231B0 ?323 3DE2338 : j3:; k“RF RES$%=38123 ?: K:38 ; 053 K32A:07; I : 8707;=07A3
15、B:8832 E:0032; I R_M I2:C63;0 7; :C:23 C3B 3B3=02E5237 231B038 I26 R_M =B3:A:C3 K30?33; ;1=B363 K9 :; 3;8C3;1 3;J 8;1=B3:3 7;ABA38 7; :EE07 U)V Zl7C123 $F l7C123 $ 5? 053 R_M I2:C63;0:07; E:0032; 09E7=:B =5:2:=0327J 07= I :EE07 ZR_M B:8832 7; “mJGWJ$ =3BB :I032 QJ8:9 l 6:C;307= I73B8 3DE1234051C5 05
16、3 :61;0 I I2:C63;07H38 R_M ?: ;0 A329 57C5 :=287;C 0 053 :EE3:238 B:8832FS; 053 =;02B =3BB ZI233 I 3DE1234 057 E3=7I7= R_M =B3:A:C3 E:0032; ?: ;0 K32A38 :0 :BBF =10,/(2*(-*( 3+*/,2*,;9 4-4192+2 ,. *(11 4;,;),2+2 89 ,(*3=50 PP*(Q 0:7;7;C ?: 138 0 7;A307C:03 053 =5:;C3 7; 053 ;1=B31 I =3BB4:;8 6:;9 :E
17、E007= K873 =;0:7;7;C ;1=B3:2 I2:C63;0 ?323 I1;8 7; l 6:C;307= I73B8 3DE38 =3BB4K10 ;3 7; 1;023:038 =3BBF S; l7C123 *4 :22? 7;87=:038 :EE007= ;1J =B3:2 I2:C63;0:07; 7; 3DE123 C21E4?57=5 ?: ;0 5?; 7; =;02B C21EFM0 053 :63 07634=90EB:67= 527;a:C3 ?: KJ 32A38 7; =3BB =1B01238 1;832 3DE123FM;8 7; 63 =3BB
18、43A3; 053 =3BB 636K2:;3 ?: 1;:KB3 0 a33E 7;0:=0F =10,/(2*(-*( 3+*/,2*,;9 4-4192+2 ,. *(11 4;,;),2+2 89 6CDEE :,081( 2)4+-+-7 N53 3DE38 :;8 =;02B “JGWJ$ =3BB ?323 0:7;38 ?705 M23=3;=3 67=2=E3 I2 053 E32=3;0J :C3 I A7:KB34:EE007= :;8 ;3=207= =3BBFMI032 l 6:C;307= I73B8 3DE1234053 E32=3;0:C3 I A7:KB3 =
19、3BB 7; 3DE123 C21E Z)F*d?: 2:0532 B? =6E:238 ?705 053 =;02B C21EZ)$FQd4 :;8 ?: :=6E:;738 ?705 : 57C5 :EE007= 2:03ZB9 ,F*d 7; =;02B C21EF M B:2C3 ;16K32 I :EE007= =3BB ?323 :0 053 3:2B9 0:C3 I :EE07F M;8 053 ;3=207= =3BB ;16K32 I 3DJ E38 =3BB Z$QF(d ?: B:2C32 05:; 05:0 I =;02B C21E Z,dF N53 231B0 K0:
20、7;38 ?705 057 81KB3 0:7;7;C ?323 23E233;038 7; l7CJ 123 PFl7C123 P 5? 053 87II323;0 62E5BC9 I =3BB 0:7;38 K9 M1KB3 0:7;7;CFN53 =;02B =3BB :EE3:238 0 K3 21;84 7;0:=0 :;8 K27C50 C233; ?57B3 63 I 053 3DE38 =3BB 3D57K7038 7223C1B:2 =3BB 62E5BC9 :;8 =;83;38 ;1=B31F FSG$SSFC5 N537;A307C:07;I6:C;307=I73B8;
21、=:;=32=3BBK3C:; I26 $)%-4:;8 7;=3 053; : B0 I 01873 :K10 053 3II3=0 I 6:C;307= I73B8 ; =:;=32 =3BB 78 + 5:A3 K33; =:22738 10 ?2B8?783 U$%VF S; 23=3;0 93:24 6:;9 233:2=532 5:A3 I=138 j iR_M 7H3 6:2a32c$i+3BB :I032 QJ8:9 3D02363B9 B? I23b13;=9 6:C;307= I73B8 3DE123c *i +;02B =3BB ZI233 I 3DE123 l7C123
22、 $n2:873;0 3B3=02E5237 I “JGWJ$ =3BB R_M Z10 I 05233 7 5?; j$* , (- KE P - KE $ *- KE Q- KE (- KE !“# ISSN 1671-5926CN 21-1470/R www.#$% 对照组细胞则在另 一个培养箱中培养,除无磁场干预外,与磁场处理组细胞的生长条件完 全一致。 在细胞培养的第K天以HYI梯度电泳、Joechst EE25K染色和 IL/MN双染色检测凋亡情况。 主要观察指标:有无HYI断裂后形成的梯度。有无异常的细胞核。 凋亡细胞的比例。 结果:HYI梯度电泳检测细胞核间HYI裂解情况jSd
23、XJMeX1细胞在 极低频磁场处理K d后产生HYI片段条带,对照组中(无磁场处理)则 未观察到此特征性HYI条带。Joechst EE25K荧光染色细胞凋亡分 析jJoechst EE25K染色被用来研究细胞中核的变化,在极低频磁场处理 过的细胞中,存在许多包含有细胞核片段的凋亡小体,但在对照组中则 几乎没有。 同时,磁场处理的细胞观察到有细胞质皱缩现象,在某些细 胞中,甚至细胞膜都不完整。IL/MN双染细胞凋亡分析j极低频磁场 处理后活细胞的比例9G2kb与对照组91GKkb相比极为低下,且伴有很高 的细胞凋亡率72GEkb,对照组细胞凋亡率为4G2kD凋亡细胞中的大多数 属于凋亡早期。
24、磁场处理后坏死细胞的比例1KG5kb也比对照组4kb要 高。IL/MN双染结果显示IL/MN双染后细胞的不同形态,其中对照组 的细胞呈亮绿色的完整圆球状, 而磁场处理后的细胞则呈现出不规则 的细胞形态且有凝集的细胞核。 结论:50 JR,20 mV极低频磁场能在体外诱导人肝癌细胞SdXJMeX1发 生凋亡。 主题词:电磁场;人;肝肿瘤;细胞凋亡 中图分类号:RE5KGE文献标识码:I文章编号:1671-59262006b25-01K4-0E 董良!黄玲珍!孙海英!陈彦田!沈阳!肖登明!齐瀚实G极低频磁场诱导人肝癌细胞的 凋亡!“#$中国临床康复!%= 1997n404bjE92-7 leng
25、TD lheng CD lhang D 97 4%8 MUperimental studies on ultraXloW freQuency pulsed gradient magnetic field inducing apoptosis of cancer cell and inhibiting groWth of cancer cellG -.+/001+ 59:19;= 2002n451bjEE-9 aang TaGRestraining solid tumor S1K0 o eEKK in mouse by magnetic fieldG ?.9/0w1 3:19 200EnE1bj
26、24-6 Hong SD Juang SlD Chen aVD 97 A%8 Mffects of eUtremely loW freQuency magnetX ic field on groWth ZineticsDmetabolism and cell cycle of human liOer cancer cellsG B.9/0w1 3:192005n5Ebj1-6 Vaga hDShiraishi dDShimura VD97 A%8pncreased freQuencies of gene and chromosome mutations after Xirradiation i
27、n mouse embryonal carcinoma cells transfected With the bclX2 geneGCD/ C A/*9E F9G 2000n9110bj994-1000 Siu JSD Chen aD Cui JD 97 A%8 Regulating eUpressions of H1D pRbD and antiX cancer effects of deguelin on human NurZitts lymphoma Haudi cells 3/ H37E+8 I*7A J.AE, B3/ 2005n267bjK7E-K0 Seite hD muinta
28、XCosta hD Seite eSD 97 A%8 Critical eOaluation of techniQues to detect and measure cell deathXstudy in a model of _q radiation of the leuZaemic cell line JS60GI/A% 9% JA7.+% 1999n19E-4bj1E9-51 Si SPG Vhe eUperimental guide for cell biologyGNeiringj China Jigher MducaX tion eress and SpringerXqerlag
29、Jeidelberg 2001j6K-75 ao gD Shan NMD Chen sD 97 A%8 Mffects of spider hacrothele raOen Oenom on cell proliferation and cytotoUicity in Jela cellsGI*7A J.AE, B3/ 2005n26Ebj E69-76 lhu mSD ain TD uo SD 97 A%8 Study on the eUpression of immediatelyX early genes and apoptosis in spinal cord ischemia and reperfusion inX ruryG K.+/001+ 23/*.1A/0 LA/0M1200En717bj2E90-1 Vofani SD Narone HD Cintorino hD 97 A%8 Static and MST magnetic fields induce tumor groWth inhibition and apoptosisG 3+79*./+% 2005n51bj12 1E 14 15 !“#