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1、a Date: 4 November 2008 Origin: International Latest date for receipt of comments: 28 February 2009 Project no.: 2007/02242 Responsible committee: AW/5 Chemical analysis of milk and milk products Interested committees: Title: Draft BS ISO 10932 Milk and milk products Determination of the minimal inh
2、ibitory concentration (MIC) of antibiotics applicable to non-enterococcal lacticacid bacteria (LAB) Supersession information: If this document is published as a standard, the UK implementation of it will supersede NONE and partially supersede. NONE If you are aware of a current national standard whi
3、ch may be affected, please notify the secretary (contact details below). WARNING: THIS IS A DRAFT AND MUST NOT BE REGARDED OR USED AS A BRITISH STANDARD. THIS DRAFT IS NOT CURRENT BEYOND 28 February 2009. This draft is issued to allow comments from interested parties; all comments will be given cons
4、ideration prior to publication. No acknowledgement will normally be sent. See overleaf for information on commenting. No copying is allowed, in any form, without prior written permission from BSI except as permitted under the Copyright, Designs and Patent Act 1988 or for circulation within a nominat
5、ing organization for briefing purposes. Electronic circulation is limited to dissemination by e-mail within such an organization by committee members. Further copies of this draft may be purchased from BSI Customer Services, Tel: +44(0) 20 8996 9001 or email . British, International and foreign stan
6、dards are also available from BSI Customer Services. Information on the co-operating organizations represented on the committees referenced above may be obtained from the responsible committee secretary. Cross-references The British Standards which implement International or European publications re
7、ferred to in this draft may be found via the British Standards Online Service on the BSI web site http:/. Direct tel: 020 8996 7009 Responsible Committee Secretary: Committee Service Centre (BSI) E-mail: Draft for Public Comment Head Office 389 Chiswick High Road London W4 4AL Telephone: +44(0)20 8
8、996 9000 Fax: +44(0)20 8996 7001 Form 36 Version 8.0 DPC: 08/30170398 DC Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI b Introduction This draft standard is based on international discussions in which the UK has taken an active part.
9、 Your comments on this draft are invited and will assist in the preparation of the consequent standard. Comments submitted will be reviewed by the relevant BSI committee before sending the consensus UK vote and comments to the international secretariat, which will then decide appropriate action on t
10、he draft and the comments received. If the international standard is approved, it is possible the text will be published as an identical British Standard. UK Vote Please indicate whether you consider the UK should submit a negative (with reasons) or positive vote on this draft. Submission The guidan
11、ce given below is intended to ensure that all comments receive efficient and appropriate attention by the responsible BSI committee. Annotated drafts are not acceptable and will be rejected. All comments must be submitted, preferably electronically, to the Responsible Committee Secretary at the addr
12、ess given on the front cover. Comments should be compatible with Version 6.0 or Version 97 of Microsoft Word for Windows, if possible; otherwise comments in ASCII text format are acceptable. Any comments not submitted electronically should still adhere to these format requirements. All comments subm
13、itted should be presented as given in the example below. Further information on submitting comments and how to obtain a blank electronic version of a comment form are available from the BSI web site at: http:/ Template for comments and secretariat observations Date: xx/xx/200x Document: ISO/DIS xxxx
14、x 1 2 (3) 4 5 (6) (7) MB Clause No./ Subclause No./ Annex (e.g. 3.1) Paragraph/ Figure/Table/ Note (e.g. Table 1) Type of com- ment Comment (justification for change) by the MB Proposed change by the MB Secretariat observations on each comment submitted 3.1 Definition 1 ed Definition is ambiguous an
15、d needs clarifying. Amend to read . so that the mains connector to which no connection . 6.4 Paragraph 2 te The use of the UV photometer as an alternative cannot be supported as serious problems have been encountered in its use in the UK. Delete reference to UV photometer. Microsoft and MS-DOS are r
16、egistered trademarks, and Windows is a trademark of Microsoft Corporation. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI THIS DOCUMENT IS A DRAFT CIRCULATED FOR COMMENT AND APPROVAL. IT IS THEREFORE SUBJECT TO CHANGE AND MAY NOT BE R
17、EFERRED TO AS AN INTERNATIONAL STANDARD UNTIL PUBLISHED AS SUCH. IN ADDITION TO THEIR EVALUATION AS BEING ACCEPTABLE FOR INDUSTRIAL, TECHNOLOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT INTERNATIONAL STANDARDS MAY ON OCCASION HAVE TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL TO BECOME STANDARDS TO
18、 WHICH REFERENCE MAY BE MADE IN NATIONAL REGULATIONS. RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT, WITH THEIR COMMENTS, NOTIFICATION OF ANY RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE AND TO PROVIDE SUPPORTING DOCUMENTATION. DRAFT INTERNATIONAL STANDARD ISO/DIS 10932 DRAFT INTERNATIONAL STANDA
19、RD ISO/IDF 223 International Organization for Standardization and International Dairy Federation, 2008 INTERNATIONAL ORGANIZATION FOR STANDARDIZATION ORGANISATION INTERNATIONALE DE NORMALISATION ISO/TC 34/SC 5 Voting begins on: 2008-10-27 Secretariat: NEN Voting terminates on: 2009-03-27 Milk and mi
20、lk products Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB) Lait et produits laitiers - Dtermination de la concentration minimale inhibitrice dantibiotiques applicable aux bifidobacteria et bactri
21、es lactiques non-entrocoques ICS 67.100.01 In accordance with the provisions of Council Resolution 15/1993 this document is circulated in the English language only. Conformment aux dispositions de la Rsolution du Conseil 15/1993, ce document est distribu en version anglaise seulement. This draft is
22、submitted to a parallel enquiry vote in ISO and IDF. To expedite distribution, this document is circulated as received from the committee secretariat. ISO Central Secretariat work of editing and text composition will be undertaken at publication stage. Pour acclrer la distribution, le prsent documen
23、t est distribu tel quil est parvenu du secrtariat du comit. Le travail de rdaction et de composition de texte sera effectu au Secrtariat central de lISO au stade de publication. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI ISO/DIS 1
24、0932|IDF 223 ii ISO and IDF 2008 All rights reserved PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the comput
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27、entral Secretariat at the address given below. Copyright notice This ISO|IDF document is a Draft International Standard and is copyright-protected by ISO and IDF. Except as permitted under the applicable laws of the users country, neither this ISO|IDF draft nor any extract from it may be reproduced,
28、 stored in a retrieval system or transmitted in any form or by any means, electronic, photocopying, recording or otherwise, without prior written permission being secured. Requests for permission to reproduce should be addressed to ISO or IDF at the respective address below. ISO copyright office Cas
29、e postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org International Dairy Federation Diamant Building Bd Auguste Reyers 80 B-1030 Brussels Tel. + 32 2 733 98 88 Fax + 32 2 733 04 13 E-mail infofil-idf.org Web www.fil-idf.org Reproduction
30、may be subject to royalty payments or a licensing agreement. Violators may be prosecuted. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI ISO/DIS 10932 | IDF 223 ISO 2008 All rights reserved iii Contents Page Foreword iv Foreword .v In
31、troductionvi 1 Scope1 2 Normative references1 3 Terms and definitions.1 4 Principle .2 5 Diluents, culture media and reagents .2 6 Apparatus and glassware.10 7 Sampling 11 8 Procedure.11 8.1 Pre-cultivation11 8.2 Quality control strains 11 8.3 Growth conditions for antimicrobial susceptibility test1
32、2 8.4 Preparation of microdilution plate.12 9 Expressions of test results 16 10 Precision 16 10.1 Interlaboratory test16 10.2 Repeatability17 10.3 Reproducibility 17 11 Test report17 Annex A (informative) Interlaboratory trial.18 Bibliography23 Licensed Copy: London South Bank University, South Bank
33、 University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI ISO/DIS 10932 | IDF 223 iv ISO 2008 All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standard
34、s is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take
35、part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. The main task of technical committees is t
36、o prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility
37、that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 10932 | IDF 223 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products a
38、nd the International Dairy Federation (IDF). It is being published jointly by ISO and IDF. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI ISO/DIS 10932 | IDF 223 ISO 2008 All rights reserved v Foreword IDF (the International Dairy Fed
39、eration) is a non-profit organization representing the dairy sector worldwide. IDF membership comprises National Committees in every member country as well as regional dairy associations having signed a formal agreement on cooperation with IDF. All members of IDF have the right to be represented at
40、the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO in the development of standard methods of analysis and sampling for milk and milk products. Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National Committe
41、es for voting. Publication as an International Standard requires approval by at least 50% of IDF National Committees casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. IDF shall not be held responsible f
42、or identifying any or all such patent rights. ISO 10932 | IDF 223 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products and the International Dairy Federation (IDF). It is being published jointly by ISO and IDF. All work was carried out by the Joint
43、ISO-IDF Action Team on Probiotics of the Standing Committee on Microbiology methods of analysis under the aegis of its project leader, Mr. M. Danielsen (SW). Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI ISO/DIS 10932 | IDF 223 vi IS
44、O 2008 All rights reserved Introduction There are several reports on MIC determination of lactic acid bacteria according to various methods. However the MIC value obtained depends on the MIC determination method used and the strain cultivation technique. For example, MIC determined by different quan
45、titative methods are not always equivalent. Also some media components are antagonistic to certain antibiotics. Considering these points, it is necessary to dispose of a normalized method for MIC determination which employs a suitable growth media having little or no antagonistic effects towards stu
46、died antibiotics. Two EU projects (PROSAFE and ACE-ART) were launched to tackle these issues, and propose appropriate media and method to measure MIC. This document is based on the SOP (standard operating procedure) proposed by ACE-ART. Licensed Copy: London South Bank University, South Bank Univers
47、ity, 31/01/2009 03:35, Uncontrolled Copy, (c) BSI DRAFT INTERNATIONAL STANDARD ISO/DIS 10932 | IDF 223 ISO 2008 All rights reserved 1 Milk and milk products Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacter
48、ia (LAB) 1 Scope This International Standard specifies a method for determining the minimal inhibitory concentration (MIC) of a series of antibiotic applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB). NOTE In contrast to the disk diffusion method, which is a semi-quantitati
49、ve method, the broth microdilution method, one of the most frequently used methods, gives quantitative MICs of the test organism into a dilution series of the antibiotics and the lowest concentration of an antibiotic that prevents visible growth of a test organism is considered as the MIC. This International Standard recommends the broth microdilution meth