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1、BS EN ISO 648:2008 ICS 17.060 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW BRITISH STANDARD Laboratory glassware Single-volume pipettes (ISO 648:2008) Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI This Briti
2、sh Standard was published under the authority of the Standards Policy and Strategy Committee on 31 October 2008 BSI 2008 ISBN 978 0 580 58559 3 Amendments/corrigenda issued since publication DateComments BS EN ISO 648:2008 National foreword This British Standard is the UK implementation of EN ISO 64
3、8:2008. It supersedes BS1583:1986 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee LBI/36/1, General and volumetric, glass and plastics apparatus. A list of organizations represented on this committee can be obtained on request to its secretary. This p
4、ublication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunity from legal obligations. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25
5、, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 648 October 2008 ICS 17.060 English Version Laboratory glassware - Single-volume pipettes (ISO 648:2008) Verrerie de laboratoire - Pipettes un volume (ISO 648:2008) Laborgerte aus Glas - Vollpipetten (ISO 648:2008)
6、 This European Standard was approved by CEN on 4 October 2008. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical ref
7、erences concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN membe
8、r into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, L
9、atvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 B
10、russels 2008 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 648:2008: E Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:2008 EN ISO 648:2008 (E) 3 F
11、oreword This document (EN ISO 648:2008) has been prepared by Technical Committee ISO/TC 48 “Laboratory glassware and related apparatus“ in collaboration with Technical Committee CEN/TC 332 “Laboratory equipment”, the secretariat of which is held by DIN. This European Standard shall be given the stat
12、us of a national standard, either by publication of an identical text or by endorsement, at the latest by April 2009, and conflicting national standards shall be withdrawn at the latest by April 2009. Attention is drawn to the possibility that some of the elements of this document may be the subject
13、 of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulga
14、ria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text
15、of ISO 648:2008 has been approved by CEN as a EN ISO 648:2008 without any modification. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:2008 ISO 648:2008(E) ISO 2008 All rights reserved iii Contents Page Foreword iv 1 Sco
16、pe . 1 2 Normative references. 1 3 Terms and definitions. 1 4 Basis of adjustment 2 4.1 Unit of volume. 2 4.2 Delivery volume 2 4.3 Reference temperature. 2 5 Types and classes of accuracy. 2 5.1 Classes of accuracy . 2 5.2 Types of single-volume pipette. 2 6 Maximum permissible errors. 3 7 Construc
17、tion 3 7.1 Material 3 7.2 Shape . 3 7.3 Bulb 3 7.4 Dimensions 4 7.5 Top of pipette 5 7.6 Delivery jet. 5 7.7 Delivery time 5 7.8 Waiting time. 6 8 Graduation line(s). 6 9 Setting of the meniscus . 6 10 Marking 6 11 Visibility of graduation lines, figures and inscriptions. 7 12 Colour coding 7 Biblio
18、graphy. 8 Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:2008 ISO 648:2008(E) iv ISO 2008 All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standa
19、rds bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organ
20、izations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given i
21、n the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 %
22、 of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 648 was prepared by Technical Committee ISO/TC 48, Laborato
23、ry equipment, Subcommittee SC 6, Laboratory and volumetric ware. This second edition cancels and replaces the first edition (ISO 648:1977), which has been technically revised to incorporate the following modifications: a) single-volume pipettes with two marks have been added; b) the waiting time for
24、 pipettes Class AS has been reduced to 5 s; c) dimensions have been modified; d) the nominal volume of 200 ml has been deleted. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:2008 INTERNATIONAL STANDARD ISO 648:2008(E) I
25、SO 2008 All rights reserved 1 Laboratory glassware Single-volume pipettes 1 Scope This International Standard specifies metrological and constructional requirements for volumetric pipettes with one mark (total delivery) and for volumetric pipettes with two marks, both of which are adequate for gener
26、al laboratory purposes. The details specified are in conformity with the principles of design and construction of volumetric glassware given in ISO 384. NOTE For graduated pipettes, see ISO 835. For piston-operated pipettes, see ISO 8655-2. 2 Normative references The following referenced documents a
27、re indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 384:1978, Laboratory glassware Principles of design and construction of volumetri
28、c glassware ISO 719, Glass Hydrolytic resistance of glass grains at 98 C Method of test and classification ISO 1769, Laboratory glassware Pipettes Colour coding ISO 3696, Water for analytical laboratory use Specification and test methods 3 Terms and definitions For the purposes of this document, the
29、 following terms and definitions apply. 3.1 delivery volume volume of liquid discharged from the pipette NOTE Due to retention of liquid on the inner surface of the volumetric instrument, the volume of liquid delivered is not identical with the volume of liquid contained by the volumetric instrument
30、. 3.2 delivery time time required for the descent of the liquid meniscus from the graduation line to the point at which the meniscus appears to come to rest in the jet for one-mark pipettes and from the upper graduation line to the lower graduation line for two-mark pipettes 3.3 waiting time time to
31、 be observed after apparent completion of the liquid delivery of the volumetric instrument NOTE A waiting time applies for Class AS single-volume pipettes (see Clause 5 and 7.8). Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN IS
32、O 648:2008 ISO 648:2008(E) 2 ISO 2008 All rights reserved 4 Basis of adjustment 4.1 Unit of volume The unit of volume shall be the millilitre (ml), which is equivalent to one cubic centimetre (cm3). 4.2 Delivery volume Single-volume pipettes shall be adjusted with water of grade 3 quality, in accord
33、ance with to ISO 3696, to deliver their nominal volume (Ex). The delivery should be under gravity and unrestricted. For details, see 7.7 and 7.8. 4.3 Reference temperature The standard reference temperature, i.e. the temperature at which the pipette is intended to deliver its nominal volume, shall b
34、e 20 C. When the pipette is required for use in a country which has adopted a standard reference temperature of 27 C (the alternative recommended in ISO 384 for tropical use), this figure shall be substituted for 20 C. 5 Types and classes of accuracy 5.1 Classes of accuracy Two classes of accuracy a
35、re specified: Class A and AS for the higher grade; Class B for the lower grade. 5.2 Types of single-volume pipette The following two types of single-volume pipettes are specified: those for which no waiting time is specified (Classes A and B); those for which a waiting time of 5 s is specified (Clas
36、s AS). Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:2008 ISO 648:2008(E) ISO 2008 All rights reserved 3 6 Maximum permissible errors Maximum permissible errors in the delivered volume shall not exceed the limits specif
37、ied in Table 1. Table 1 Nominal volumes and maximum permissible errors Maximum permissible error Nominal volume Class A and AS Class B ml ml ml 0,5 0,005 0,010 1 0,008 0,015 2 0,010 0,02 5 0,015 0,03 10 0,02 0,04 20 0,03 0,06 25 0,03 0,06 50 0,05 0,10 100 0,08 0,15 The relationships between maximum
38、permissible error and nominal volume, as well as between maximum permissible error and meniscus diameter as given in ISO 384:1978, Annex A and Annex B, shall be observed. For the maximum permissible errors of single-volume pipettes with intermediate nominal volumes between those given in Table 1, th
39、e value for the nearest nominal volume shall apply. If the intermediate volume is exactly halfway between those given in Table 1, the value for the next greater nominal volume shall apply. 7 Construction 7.1 Material Single-volume pipettes shall be manufactured from glass of chemical resistance and
40、thermal properties at least to HGB3 in accordance with ISO 719, shall be as free as possible from visible defects and reasonably free from internal stress. 7.2 Shape Pipettes of 0,5 ml nominal volume shall consist of a straight tube with a jet at the lower end. Pipettes of nominal volume 1 ml and 2
41、ml may be of similar straight tube pattern or may alternatively be of bulb pattern, as described herein for the larger sizes. All larger pipettes shall consist of a bulb with suction and delivery tubes, the three portions being straight and coaxial. 7.3 Bulb The main portion of the bulb shall be cyl
42、indrical, except that 1 ml and 2 ml nominal-volume pipettes may have an oval bulb if desired. The two ends of the bulb shall merge gradually into the suction and delivery tubes, the junctions being of tapered formation to facilitate drainage. Licensed Copy: London South Bank University, South Bank U
43、niversity, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:2008 ISO 648:2008(E) 4 ISO 2008 All rights reserved 7.4 Dimensions Single-volume pipettes shall comply with the dimensional requirements shown in Tables 2, 3 and 4. Table 2 Dimensions Nominal volume ml Dimensions mm 0,5 1 2 5 10 2
44、0 25 50 100 Overall length max. 300 325 350 410 450 520 530 550 600 Internal diameter at the graduation line max. 2,5 3,5 4,5 4,5 5 5,5 5,5 6 7,5 Length of suction tube valid only for bulb pattern pipettes min. 150 150 150 160 170 170 170 170 Length of delivery tube valid only for bulb pattern pipet
45、tes min. 110 125 145 160 210 220 230 240 External diameter of delivery tube valid only for bulb pattern pipettes 1 5 6 6,5 7 7 7 8 9 External diameter of bulb valid only for bulb pattern pipettes max. 9 9 12 16 22 24 30 38 External diameter of tube valid only for straight pattern pipettes max. 5 6 7
46、 Table 3 Dimensions applying to all nominal volumes of one-mark pipettes Dimension Size mm Distance of graduation line from top of pipette min. 100 Distance from graduation line to top of bulba min. 10 Distance from graduation line to tip of jet valid only for straight pattern pipettes min. 110 Wall
47、 thickness bulb pattern pipettes straight pattern pipettes min. min. 0,6 0,8 a Top of bulb relates to the point at which the suction tube begins to expand into the bulb. Licensed Copy: London South Bank University, South Bank University, 31/01/2009 03:25, Uncontrolled Copy, (c) BSI BS EN ISO 648:200
48、8 ISO 648:2008(E) ISO 2008 All rights reserved 5 Table 4 Dimensions applying to all nominal volumes of two-mark pipettes Dimension Size mm Distance of upper graduation line from top of pipette min. 100 Distance from upper graduation line to top of bulba min. 10 Distance from upper graduation line to
49、 tip of jet valid only for straight pattern pipettes min. 110 Distance from lower graduation line to bottom of bulba min. 20 Distance from lower graduation line to tip of jet min. 30 Wall thickness bulb pattern pipettes straight pattern pipettes min. min. 0,6 0,8 a Top of bulb and bottom of bulb relate to the points at which the suction tube and the delivery tube begin to expand into the bulb. 7.5 Top of pipette The top of the single-volume pipette shall lie in a pla