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1、BRITISH STANDARD BS EN ISO 11290-2:1998 Incorporating Amendment No. 1 Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenes Part 2: Enumeration method The European Standard EN ISO 11290-2:1998, with the incorporation of amendment
2、 A1:2004, has the status of a British Standard ICS 07.100.30 ? Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN ISO 11290-2:1998 This British Standard, having been prepared under the direction of the Consumer Products and Services Secto
3、r Board, was published under the authority of the Standards Board and comes into effect on 15 September 1998 BSI 12 January 2005 ISBN 0 580 30208 3 National foreword This British Standard is the English language version of EN ISO 11290-2:1998, including amendment A1:2004. It is identical with ISO 11
4、290-2:1998, including amendment 1:2004. The start and finish of text introduced or altered by ISO amendment is indicated in the text by tags !“. Tags indicating changes to ISO text carry the number of the ISO amendment. For example, text altered by ISO amendment 1 is indicated by !“. The UK particip
5、ation in its preparation was entrusted to Technical Committee AW/9, Microbiology, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publi
6、cations referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue of British Standards Online. This publication does not purport to include all the necess
7、ary provisions of a contract. Users are responsile for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpr
8、etation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN ISO title page, the EN ISO foreword page, the ISO title p
9、age, pages ii to iv, pages 1 to 19 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. DateComments 1539212 January 2005See national foreword Licensed Copy: sheffieldun sheffieldun, na, Thu No
10、v 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11290-2 July 1998 + A1 October 2004 ICS 07.100.00 Descriptors: See ISO document English version Microbiology of food and animal feeding stuffs Horizontal method for the detection and enu
11、meration of Listeria monocytogenes Part 2: Enumeration method (includes amendment A1:2004) (ISO 11290-2:1998 + A1:2004) Microbiologie des aliments Mthode horizontale pour la recherche et le dnombrement de Listeria monocytogenes Partie 2: Mthode de dnombrement (inclut lamendement A1:2004) (ISO 11290-
12、2:1998 + A1: 2004) Mikrobiologie von Lebensmitteln und Futtermitteln Horizontales Verfahren fr den Nachweis und die Zhlung von Listeria monocytogenes Teil 2: Zhlverfahren (enthlt nderung A1:2004) (ISO 11290-2:1998 + A1:2004) This European Standard was approved by CEN on 21 June 1998; amendment A1 wa
13、s approved by CEN on 30 September 2004. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such
14、 national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language a
15、nd notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
16、Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. CEN European Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1998 CEN All rights of exploitat
17、ion in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11290-2:1998 + A1:2004 E Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 11290-2:1998 BSI 12 January 2005 Foreword The text of the Internati
18、onal Standard ISO 11290-2:1998 has been prepared by Technical Committee ISO/TC 34, Agricultural food products, in collaboration with Technical Committee CEN/TC 275, Food analysis Horizontal methods, the Secretariat of which is held by DIN. This European Standard shall be given the status of a nation
19、al standard, either by publication of an identical text or by endorsement, at the latest by January 1999, and conflicting national standards shall be withdrawn at the latest by January 1999. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following coun
20、tries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of the International St
21、andard ISO 11290-2:1998 was approved by CEN as a European Standard without any modification. NOTENormative references to International Standards are listed in Annex ZA (normative). Foreword to amendment A1 This document (EN ISO 11290-2:1998/A1:2004) has been prepared by Technical Committee ISO/TC 34
22、, Agricultural food products, in collaboration with Technical Committee CEN/TC 275, Food analysis Horizontal methods, the Secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the
23、 latest by April 2005, and conflicting national standards shall be withdrawn at the latest by April 2005. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech
24、Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of the ISO 11290-2:1998 was
25、approved by CEN as EN ISO 11290-2:1998/A1:2004 without any modifications. Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI Amendment 1 2004-10-15 Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Cop
26、y, (c) BSI EN ISO 11290-2:1998 ii Contents Page Forewordiii Introductioniv 1Scope1 2Normatives references1 3Definitions1 4Principle1 5Culture media and reagents2 6Apparatus and glassware2 7Sampling2 8Preparation of test sample3 9Procedure3 10Expression of results (see ISO 7218)7 11Precision8 12Quali
27、ty control of culture media8 13Test report8 13Bibliography18 Annex A (normative) Diagram of procedure9 Annex B (normative) Composition and preparation of media and reagents9 Annex C (informative) Henry illumination test16 Annex ZA (normative) Normative references to international publications with t
28、heir relevant European publications19 Figure 1 Inoculation and interpretation of CAMP test plates6 Figure C.1 Examination of plates for suspect colonies17 Table 1 Reactions for the identification of Listeria spp.6 Descriptors: Agricultural products, food, food products, animal feeding products, biol
29、ogical tests, microbiological analysis, counting, bacteria, listeria, bacteria count methods. Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 11290-2:1998 BSI 12 January 2005 iii Foreword ISO (the International Organization for Standa
30、rdization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right t
31、o be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft Internation
32、al Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 11290-2 was prepared by Technical Committee ISO/TC 34, Agricult
33、ural food products, Subcommittee SC 9, Microbiology. ISO 11290 consists of the following parts, under the general title Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenes: Part 1: Detection method; Part 2: Enumeration method.
34、Annex A and Annex B form an integral part of this part of ISO 11290. Annex C is for information only. Foreword to amendment 1:2004 ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International
35、Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liasion with ISO, al
36、so take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committ
37、ees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the poss
38、ibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Amendment 1 to ISO 11290-2:1998 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology. The
39、enumeration medium has been modified. Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN ISO 11290-2:1998 iv BSI 12 January 2005 Introduction Because of the large variety of food and feed products, this horizontal method may not be approp
40、riate in every detail for certain products. In this case, different methods, which are specific to these products may be used if absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this horizontal method as far as possible. When this part of ISO
41、11290 is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has been followed and the reasons for deviations from this method in the case of particular products. The harmonization of test methods cannot be immediate, and for ce
42、rtain groups of products International Standards and/or national standards may already exist that do not comply with this horizontal method. It is hoped that when such standards are reviewed they will be changed to comply with this part of ISO 11290 so that eventually the only remaining departures f
43、rom this horizontal method will be those necessary for well-established technical reasons. WARNING In order to safeguard the health of laboratory personnel, it is strongly recommended that tests for detecting Listeria monocytogenes are undertaken in properly equipped laboratories, under the control
44、of a skilled microbiologist, and that great care is taken in the disposal of all contaminated materials. In particular, it is strongly recommended that female laboratory staff are made aware of the particular risk to the developing foetus presented by infection of the mother through exposure to List
45、eria monocytogenes. National legislation may involve more specific demands. Licensed Copy: sheffieldun sheffieldun, na, Thu Nov 16 07:23:51 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 11290-2:1998 BSI 12 January 2005 1 1 Scope This part of ISO 11290 specifies a horizontal method for the enumer
46、ation of Listeria monocytogenes. NOTEThe method also allows enumeration of other Listeria species which may be used as indicators of the hygienic quality of food or feed products. Subject to the limitations discussed in the introduction, this part of ISO 11290 is applicable to products intended for
47、human consumption or animal foodstuffs. In general (see note in 9.2.1), the lower limit of enumeration of this method is 10 L. monocytogenes per millilitre of sample for liquid products, or 100 L. monocytogenes per gram of sample for other products. 2 Normative references The following standards con
48、tain provisions which, through reference to the text, constitute provisions of this International Standard. At the time of publication, the editions indicated were valid. All Standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investiga
49、te the possibility of applying the most recent editions of standards indicated below. Members of IEC and ISO maintain registers of currently valid International Standards. ISO 6887-1:1), Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 1: General rules for the preparation of the initial suspension and of decimal dilut