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1、BRITISH STANDARD BS EN ISO 835:2007 Laboratory glassware Graduated pipettes The European Standard EN ISO 835:2007 has the status of a British Standard ICS 17.060 ? Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BS
2、I BS EN ISO 835:2007 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2007 BSI 2007 ISBN 978 0 580 50762 5 National foreword This British Standard was published by BSI. It is the UK implementation of EN ISO 835:2007. It supersedes BS 70
3、0-1:1982, BS 700-2:1982 and BS 700-3:1982, which are withdrawn. The UK participation in its preparation was entrusted by Technical Committee LBI/36, Laboratory glassware and related apparatus, to Subcommittee LBI/36/1, General and volumetric, glass and plastics apparatus. A list of organizations rep
4、resented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunity from legal obligations. Amendm
5、ents issued since publication Amd. No. DateComments Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 835 April 2007 ICS 17.060 English Version Laboratory
6、glassware - Graduated pipettes (ISO 835:2007) Verrerie de laboratoire - Pipettes gradues (ISO 835:2007)Laborgerte aus Glas - Messpipetten (ISO 835:2007) This European Standard was approved by CEN on 13 March 2007. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipul
7、ate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard e
8、xists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standard
9、s bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdo
10、m. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2007 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 835:2007: E Lice
11、nsed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI Foreword This document (EN ISO 835:2007) has been prepared by Technical Committee ISO/TC 48 “Laboratory glassware and related apparatus“ in collaboration with Technic
12、al Committee CEN/TC 332 “Laboratory equipment“, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2007, and conflicting national standards shall be w
13、ithdrawn at the latest by October 2007. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Gr
14、eece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 835:2007 has been approved by CEN as EN ISO 835:2007 without any modific
15、ations. EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI Reference number ISO 835:2007(E) INTERNATIONAL STANDARD ISO 835 First edition 2007-04-01 Laboratory glassware Graduated pipettes Verrerie
16、de laboratoire Pipettes gradues EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI ii Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007,
17、Uncontrolled Copy, (c) BSI iii Contents Page Foreword iv 1 Scope . 1 2 Normative references. 1 3 Terms and definitions. 1 4 Basis of adjustment 2 4.1 Unit of volume. 2 4.2 Delivery volume 2 4.3 Reference temperature. 2 5 Types and classes of accuracy. 2 5.1 Classes of accuracy . 2 5.2 Types of pipet
18、tes 2 6 Maximum permissible errors. 3 7 Construction 4 7.1 Material 4 7.2 Dimensions 4 7.3 Top of pipette 4 7.4 Delivery jet. 4 7.5 Delivery time 5 7.6 Waiting time. 5 8 Graduation, figuring and patterns. 5 8.1 Graduation patterns 5 8.2 Position of graduation lines 5 8.3 Figuring of graduation lines
19、 5 9 Setting of the meniscus . 6 10 Marking 6 11 Visibility of graduation lines, figures and inscriptions. 7 12 Colour coding 7 Annex A (normative) Definition of capacities and delivery times. 8 Bibliography. 12 EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank Universit
20、y, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technic
21、al committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely wi
22、th the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft Inte
23、rnational Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document ma
24、y be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 835 was prepared by Technical Committee ISO/TC 48, Laboratory equipment, Subcommittee SC 6, Laboratory and volumetric ware. This first edition of ISO 835 cancels and replaces ISO 8
25、35-1:1981, ISO 835-2:1981, ISO 835-3:1981 and ISO 835-4:1981, which have been technically revised and combined into one document. EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI 1 Laboratory gla
26、ssware Graduated pipettes 1 Scope This International Standard specifies metrological and constructional requirements for graduated pipettes, adequate for general laboratory purposes. The details specified are in conformity with the principles of design and construction of volumetric glassware given
27、in ISO 384. NOTE For one-mark pipettes, see ISO 648. For piston-operated pipettes, see ISO 8655-2. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the lates
28、t edition of the referenced document (including any amendments) applies. ISO 384:1978, Laboratory glassware Principles of design and construction of volumetric glassware ISO 719, Glass Hydrolytic resistance of glass grains at 98 C Method of test and classification ISO 1769, Laboratory glassware Pipe
29、ttes Colour coding ISO 3696, Water for analytical laboratory use Specification and test methods 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 delivery volume volume of liquid discharged from the pipette NOTE Due to retention of liquid on th
30、e inner surface of the pipette, the volume of liquid delivered is not identical with the volume of liquid contained by the pipette. 3.2 delivery time time required for the pipette to deliver its nominal volume 3.3 waiting time time to be observed after apparent completion of the liquid delivery of t
31、he pipette and before the final reading of the delivered volume is taken NOTE A waiting time applies for graduated pipettes of Class AS (see 5.1 and 7.6). EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy,
32、 (c) BSI 2 4 Basis of adjustment 4.1 Unit of volume The unit of volume shall be the millilitre (ml) which is equivalent to the cubic centimetre (cm3). 4.2 Delivery volume Graduated pipettes shall be adjusted with water according to ISO 3696, grade 3, to deliver their specified volume (Ex). The deliv
33、ery should be under gravity and unrestricted. For details, see 7.5. 4.3 Reference temperature The standard reference temperature, i.e. the temperature at which the pipette is intended to deliver its volume, shall be 20 C. When the pipette is required for use in a country which has adopted a standard
34、 reference temperature of 27 C (the alternative recommended in ISO 384 for tropical use), this figure shall be substituted for 20 C. 5 Types and classes of accuracy 5.1 Classes of accuracy Two classes of accuracy are specified: Classes A and AS for the higher grade; Class B for the lower grade. The
35、maximum permissible errors for both classes are specified in Table 1. The pipettes shall be adjusted as follows: Class A: without waiting time; Class AS: with a specified waiting time of 5 s (see 7.6); Class B: without waiting time. 5.2 Types of pipettes 5.2.1 Type 1: Partial delivery Graduated pipe
36、ttes adjusted for delivery of a liquid from zero line at the top to any graduation line; nominal capacity shall be represented by the lowest graduation line. 5.2.2 Type 2: Total delivery Graduated pipettes adjusted for delivery of a liquid from any graduation line down to the jet; nominal capacity s
37、hall be represented by the highest graduation line. EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35 GMT+00:00 2007, Uncontrolled Copy, (c) BSI 3 5.2.3 Type 3: Total delivery Graduated pipettes adjusted for delivery of a liquid from zero
38、line at the top to any graduation line; nominal capacity shall be obtained by delivery down to the jet. 5.2.4 Type 4: Blow-out Graduated pipettes for total delivery (see Type 3) where the last drop of liquid in the jet is expelled by blowing. Blow-out pipettes shall be adjusted to accuracy Class B.
39、6 Maximum permissible errors Maximum permissible errors in the delivered volume shall not exceed the limits specified in Table 1. These limits represent the maximum permitted error at any point and also the maximum permissible difference between the errors at any two points. Table 1 Capacities, subd
40、ivisions and maximum permissible errors Nominal capacity Smallest scale division Maximum permissible error ml ml Classes A and AS ml Class B ml 0,1 0,01 0,006 0,01 0,2 0,01 0,006 0,01 0,5 0,01 0,006 0,01 1 0,01 0,007 0,01 1 0,10 0,007 0,01 2 0,02 0,010 0,02 2 0,10 0,010 0,02 5 0,05 0,030 0,05 5 0,10
41、 0,030 0,05 10 0,1 0,05 0,1 20 0,1 0,1 0,2 25 a 0,1 0,1 0,2 25 0,2 0,1 0,2 a Length 450 mm, see Table 2. If graduated pipettes are required of capacities and/or subdivisions other than those listed in Table 1, they shall conform to the essential requirements of this International Standard. The relat
42、ionships between maximum permissible error and capacity, as well as between maximum permissible error and meniscus diameter as given in ISO 384:1978, Annex A and Annex B, shall be observed. EN ISO 835:2007 Licensed Copy: London South Bank University, London South Bank University, Fri Jun 01 01:37:35
43、 GMT+00:00 2007, Uncontrolled Copy, (c) BSI 4 7 Construction 7.1 Material Graduated pipettes shall be manufactured from glass of chemical resistance and thermal properties which are at least to class HGB3 in accordance with ISO 719, and shall be as free as possible from visible defects and shall be
44、reasonably free from internal stress. 7.2 Dimensions Graduated pipettes shall comply with the essential dimensional requirements shown in Table 2. Table 2 Dimensions Nominal capacity ml Dimensions mm 0,1 0,2 0,5 1 2 5 10 20 25 25 Smallest scale division ml 0,010,010,010,01 0,10 0,02 0,10 0,05 0,10 0
45、,1 0,1 0,1 0,2 Essential dimensions Distance from zero line to lowest graduation line for Type 1 pipettes min. max. 80 170 120 200 130 220 160 220 160 220 140 220 140 220 140 220 220 290 180 220 Distance from highest to lowest figured graduation lines for Types 2, 3 and 4 pipettes min. max. 70 160 1
46、10 190 110 220 140 220 140 220 140 220 140 220 140 220 200 290 160 220 Distance from highest graduation line to top of pipette min. 100 External diameter of suction tube approx. 5,25 to 9 5,5 to 9 Length of tube of uniform bore below lowest graduation line min. 5 Recommended dimensions Overall lengt
47、h approx. 300 340 360 360 360 360 360 360 450 360 Length of tapered portion forming jet approx. 15 15 20 20 20 25 25 30 30 30 Wall thickness approx. 2 2 2 2 1,5 1 1 1 1 1 7.3 Top of pipette The top of the graduated pipette shall lie in a plane at right angles to the axis of the pipette and shall be
48、free from any blemishes which might interfere with the required accurate control by a mechanical device or by the finger, in setting the meniscus. The end may be lightly fire-polished or smoothly ground with a slight bevel on the outside. The upper end of the pipette tube may be made from tubing of
49、uniform bore or may have a constriction at about 25 mm below the top to enable the retention of a porous, air-permeable plug, such as a cotton plug. 7.4 Delivery jet The lower end of the graduated pipette shall terminate either in a delivery jet having a smooth and gradual taper or in a capillary end, both without sudden constriction at the orifice which could give rise to turbulent outflow. The end of the jet may be finished by grinding or machine tooling and may