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1、BRITISH STANDARD BS EN ISO 14673-1:2001 Incorporating Corrigendum No. 1 Milk and milk products Determination of nitrate and nitrite contents Part 1: Method using cadmium reduction and spectrometry The European Standard EN ISO 14673-1:2001 has the status of a British Standard ICS 67.100 NO COPYING WI
2、THOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN ISO 14673-1:2001 This British Standard, having been prepared under the direction of the Consumer Products and Services Sector Pol
3、icy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 3 December 2001 BSI 12 March 2002 ISBN 0 580 38747 X National foreword This British Standard is the official English language version of EN ISO 14673-1:2001. It is identical with ISO 14673
4、-1:2001 The UK participation in its preparation was entrusted to Technical Committee AW/5, Chemical analysis of milk and milk products, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Sta
5、ndards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Stand
6、ard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the res
7、ponsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front c
8、over, the EN ISO title page, the EN ISO foreword page, the ISO title page, pages ii to v, a blank page, pages 1 to 13, a blank page, the Annex ZA page and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. Amendments issued since publication
9、Amd. No. DateComments 13739 Corr. No. 1 12 March 2002 Incorporation of Annex ZA Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 14673-1 November 2001 ICS 67.100.01 English version Milk
10、 and milk products - Determination of nitrate and nitrite contents - Part 1: Method using cadmium reduction and spectrometry (ISO 14673-1:2001) Lait et produits laitiers - Dtermination des teneurs en nitrates et en nitrites - Partie 1: Mthode par rduction au cadmium et spectromtrie (ISO 14673-1:2001
11、) Milch und Milchprodukte - Bestimmung des Nitrat- und Nitritgehaltes - Teil 1: Verfahren mit Cadmiumreduktion und Spektrometrie (ISO 14673-1:2001) This European Standard was approved by CEN on 1 November 2001. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate
12、 the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Management Centre or to any CEN member. This European Standard exists i
13、n three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official versions. CEN members are the national standards bodies of
14、 Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Manage
15、ment Centre: rue de Stassart, 36 B-1050 Brussels 2001 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 14673-1:2001 E Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI I NE
16、SO 41-376:1002( 1E) 2 CORRECTED 2002-02-06 Foreword This document (ISO 14673-1:2001) has been prepared by Technical Committee ISO/TC 34 “Agricultural food products“ in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of sampling and analysis“, the secretariat of wh
17、ich is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2002, and conflicting national standards shall be withdrawn at the latest by May 2002. According to the CEN/CENELEC Internal
18、 Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and
19、 the United Kingdom. Endorsement notice The text of the International Standard ISO 14673-1:2001 has been approved by CEN as a European Standard without any modifications. NOTE Normative references to International Standards are listed in annex ZA (normative). EN ISO 146731:2001 2 Licensed Copy: shef
20、fieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI Reference numbers ISO 14673-1:2001(E) IDF 189-1:2001(E) INTERNATIONAL STANDARD ISO 14673-1 IDF 189-1 First edition 2001-11-01 Milk and milk products Determination of nitrate and nitrite contents Part 1: Method us
21、ing cadmium reduction and spectrometry Lait et produits laitiers Dtermination des teneurs en nitrates et en nitrites Partie 1: Mthode par rduction au cadmium et spectromtrie EN ISO 146731:2001 Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI
22、ii EN ISO 146731:2001 Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI iii ContentsPage Foreword.iv 1Scope 1 2Normative references1 3Terms and definitions .2 4Principle2 5Reagents.2 6Apparatus.4 7Sampling.5 8Preparation of test sample5 8.1Dri
23、ed milk and dried whey5 8.2Caseins and caseinates 5 8.3Cheese 5 8.4Whey cheese6 9Procedure.6 9.1Preparation of the copperized cadmium column.6 9.2Checking the reducing capacity of the column6 9.3Regeneration of the column.7 9.4Preparation of test portion7 9.5Extraction and deproteination8 9.6Reducti
24、on of nitrate to nitrite .8 9.7Blank test8 9.8Determination.9 9.9Preparation of calibration graph9 10Calculation and expression of results.9 10.1Nitrite content.9 10.2Nitrate content10 11Precision.10 11.1General10 11.2Repeatability.10 12Test report11 Bibliography13 EN ISO 146731:2001 Licensed Copy:
25、sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carri
26、ed out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. I
27、SO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical commit
28、tees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this part of ISO 14673?IDF 189 may be the subject of patent rights.
29、 ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO 14673-1?IDF 189-1 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with
30、AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International. This first edition of ISO 14673-1?IDF 189-1, together with ISO 14673-2?IDF 189-2 and ISO 14673-3?IDF 189-3, cancels and replaces ISO 4099:1984, ISO 6736:1982, ISO 6739:1988, ISO 6740:1985 and ISO 8
31、195:1987, which have been technically revised. ISO 14673?IDF 189 consists of the following parts, under the general title Milk and milk products Determination of nitrate and nitrite contents: Part 1: Method using cadmium reduction and spectrometry Part 2: Method using segmented flow analysis (Routin
32、e method) Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method) EN ISO 146731:2001 Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI v Foreword IDF (the International Dairy Federation) is a w
33、orldwide federation of the dairy sector with a National Committee in every member country. Every National Committee has the right to be represented on the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO and AOAC International in the development of standard methods
34、of analysis and sampling for milk and milk products. Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National Committees for voting. Publication as an International Standard requires approval by at least 50 % of National Committees casting a vo
35、te. International Standard ISO 14673-1?IDF 189-1 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC International. It is being published jointly by ISO and IDF and separatel
36、y by AOAC International. All work was carried out by the Joint ISO/IDF/AOAC Action Team, Minerals and minor compounds, of the Standing Committee on Minor components characterization of physical properties, under the aegis of its project leader Mr G. Brthen (NO). EN ISO 146731:2001 Licensed Copy: she
37、ffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 146731:2001 Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI INTERNTAIONLA STNADARD ISO -376411:1002(E) DIF 1891-2:001(E) ISO dna IDF 1002 All rig
38、ths resreved1 Milk and milk products Determination of nitrate and nitrite contents Part 1: Method using cadmium reduction and spectrometry WARNING The use of this International Standard may involve hazardous materials, operations and equipment. This standard does not purport to address all the safet
39、y problems associated with its use. It is the responsibility of the user of this standard to establish safety and health practices and determine the applicability of regulatory limitations prior to use. 1Scope This part of ISO 14673?IDF 189 specifies a method for the determination of the nitrate and
40、 nitrite contents of milk and milk products by cadmium reduction and spectrometry. The method is applicable to ?whole and partly skimmed and skimmed dried milk; ?hard, semi-hard and soft cheeses; ?processed cheese; ?whey cheese, caseins and caseinates and dried whey. The method may be performed usin
41、g automatic equipment, in particular by segmented flow analysis (SFA) or flow injection analysis (FIA), thus reducing cadmium contamination in laboratory work places and waste water. NOTEThese methods are described in ISO 14673-2?IDF 189-2 and ISO 14673-3?IDF 189-3, respectively. 2Normative referenc
42、es The following normative documents contain provisions which, through reference in this text, constitute provisions of this part of ISO 14673?IDF 189. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on thi
43、s part of ISO 14673?IDF 189 are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently
44、valid International Standards. ISO 565, Test sieves Metal wire cloth, perforated metal plate and electroformed sheet Nominal sizes of openings ISO 648, Laboratory glassware One-mark pipettes ISO 835-1, Laboratory glassware Graduated pipettes Part 1: General requirements ISO 1042, Laboratory glasswar
45、e One-mark volumetric flasks EN ISO 146731:2001 1 Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 15:25:42 GMT+00:00 2006, Uncontrolled Copy, (c) BSI ISO -376411:(1002E) IDF 981-1:1002(E) 2 ISO dna IDF 1002 All rigths resreved 3Terms and definitions For the purposes of this part of ISO 14673?
46、IDF 189, the following terms and definitions apply. 3.1 nitrate content mass fraction of nitrate determined by the procedure specified in this part of ISO 14673?IDF 189 NOTEThe nitrate content is expressed as the mass in milligrams of nitrate ions (NO3) per kilogram of product. 3.2 nitrite content m
47、ass fraction of nitrite determined by the procedure specified in this part of ISO 14673?IDF 189 NOTEThe nitrite content is expressed as the mass in milligrams of nitrite ions (NO2) per kilogram of product. 4Principle A test portion is dispersed in warm water, with precipitation of the fat and protei
48、ns, then filtration. The nitrate ions are reduced to nitrite ions in a portion of the filtrate by means of copperized cadmium. A red colour is developed in portions of both unreduced filtrate and the reduced solution, by addition of sulfanilamide and N-1-naphthyl ethylenediamine dihydrochloride. Spe
49、ctrometric measurements are carried out at a wavelength of 538 nm. The nitrite content of the sample and the total nitrite content after reduction of nitrate ions are calculated by comparing the measured absorbances with those of a set of sodium nitrite calibration solutions. The nitrate content is calculated from the difference between these two contents. 5Reagents Use only reagents of recognized analytical grade unless otherwise specified. 5.1Water, distilled or deionized, or water of equivalent purity, free from