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1、INTERNATIONAL STANDARD IS0 6320:2000 TECHNICAL CORRIGENDUM 1 Published 2006-03-1 5 INTERNATIONAL ORGANIZATION FOR STANDARDIZATION MWWAPOflHAR OPTAHHIHR no CTAHflAPTHIAUHH ORGANISATION INTERNATIONALE DE NORMALISATION Animal and vegetable fats and oils - Determination of refractive index TECHNICAL COR
2、RIGENDUM 1 Corps gras dorigines animale et vgtale - Dtermination de lindice de rfraction RECTIFICATIF TECHNIQUE I Technical Corrigendum 1 to IS0 6320:2000 was prepared by Technical Committee ISOTC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils. Page 5 Replace Annex A by th
3、e following page. In particular, the values for the repeatability coefficient of variation and reproducibility coefficient of variation have been changed. ICs 67.200.10 Ref. No. IS0 6320:2000/Cor.l:2006(E) O IS0 2006 -All rights reserved Published in Switzerland Copyright International Organization
4、for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- IS0 6320:2000/Cor.l:2006(E) Annex A (norm at ive) Results of an interlaboratory test A national colla
5、borative test involving nine laboratories in Germany was carried out on five samples. The statistical evaluation was performed in accordance with IS0 5725-1 and IS0 5725-2 to give the precision data shown in Table A. 1. Table Al - Results of interlaboratory test Number of participating laboratories
6、Number of laboratories retained after eliminating outliers Number of individual test results of all laboratories on each sample Mean value Repeatability standard deviation, s, Repeatability coefficient of variation, % Repeatability limit, Y (= sr x 2,8) Reproducibility standard deviation, S, Reprodu
7、cibility coefficient of variation, % Reproducibility limit, R (= SR x 2,8) Rape seed oil 9 9 45 1,473 24 0,000 06 0,004 0,000 17 0,000 27 0,018 0,000 75 Sunflower seed oil 9 9 45 1,475 12 0,000 06 0,004 0,000 17 0,000 30 0,020 0,000 84 Sample Modified linseed oil 9 9 45 1,482 33 0,000 06 0,004 0,000
8、 17 0,000 33 0,022 0,000 94 Modified castor oil 9 9 45 1,483 91 0,000 05 0,003 0,000 15 0,000 40 0,027 0,001 12 Castor oil 9 9 45 1,479 30 0,000 05 0,003 0,000 13 0,000 35 0,024 0,000 98 2 O IS0 2006 -All rights reserved Copyright International Organization for Standardization Provided by IHS under
9、license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- STD-IS0 b320-ENGL 2000 B 48537U3 OBLb113 537 INTERNATIONAL STAN DARD IS0 6320 Fourth edition 2000-03-1 5 Animal and vegetable fats and oils - D
10、etermination of refractive index Corps gras dorigines animale et vgtale - Dtermination de lindice de rfraction This material is reproduced from IS0 documents under International Organization for Standardization (ISO) Copyright License Number HIS/CC/1996. Not for resale. No part of these IS0 document
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12、0 6320:2000(E) 0 IS0 2000 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- IS0 6320:2000(E) I PDF disclaimer This
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18、e=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- Foreword IS0 (the International Organization for Standardization) is a worldwide federation of national standards bodies (IS0 member bodies). The work of preparing Int
19、ernational Standards is normally carried out through IS0 technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison w
20、ith ISO, also take part in the work. IS0 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ICO/IEC Directives, Part 3. Draft International Sta
21、ndards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some o f the elements of this International Standard
22、may be the subject of patent rights. IS0 shall not be held responsible for identifying any or all such patent rights. International Standard IS0 6320 was prepared by Technical Committee ISO/TC 34, Agricultural food products, Subcommittee SC 1 1, Animal and vegetable fats and oils. This fourth editio
23、n cancels and replaces the third edition (IS0 6320:1995), which has been revised by the addition of precision data. Annex A of this International Standard is for information only. O IS0 2000 -All rights reserved . 111 Copyright International Organization for Standardization Provided by IHS under lic
24、ense with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- _ _ _ _ _ _ _ _ _ INTERNATIONAL STANDARD IS0 6320:2000(E) Animal and vegetable fats and oils - Determination of refractive index 1 Scope This Inte
25、rnational Standard specifies a method for the determination of the refractive index of animal and vegetable fats and oils. 2 Normative reference The following normative document contains provisions which, through reference in this text, constitute provisions of this International Standard. For dated
26、 references, subsequent amendments to, or revisions of, this publication do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent edition of the normative document indicated below. For undated referenc
27、es, the latest edition of the normative document referred to applies. Members of IS0 and IEC maintain registers of currently valid International Standards. IS0 661, Animal and vegfable fats and oils - Preparation of test sample. 3 Terms and definitions For the purposes of this International Standard
28、, the following term and definition apply. 3.1 refractive index (of a medium) ratio of the velocity of light of a definite wavelength in a vacuum to its velocity in the medium NOTE 1 selected wavelength is the mean wavelength of the sodium D lines (589,6 nm). In practice, the velocity of light in ai
29、r is used in place of that in a vacuum and, unless otherwise specified, the NOTE 2 notation used nb, where r is the temperature in degrees Celsius. The refractive index of a given substance varies with the wavelength of the incident light and with temperature. The 4 Principle By means of a suitable
30、refractometer, the refractive index of a liquid sample is measured at a specified temperature. 5 Reagents Use only reagents of recognized analytical grade, and distilled or demineralized water or water of equivalent purity. 5.1 Ethyl laurate, of quality suitable for refractometry, and of known refra
31、ctive index. O IS0 2000 -All rights reserved 1 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- STD-IS0 b320-ENGL
32、 2000 E 485L703 O8LbLL7 102 9 IS0 632O:2OoO( E) 5.2 the refractometer. Hexane, or other suitable solvents, such as light petroleum, acetone or toluene, for cleaning the prism of 6 Apparatus Usual laboratory equipment and, in particular, the following. 6.1 f 0,000 1 over the range n = 1,300 to n = 1,
33、700. 6 . 2 Light source: sodium vapour lamp White light can also be used if the refractometer is fitted with an achromatic compensation system. Refractometer, for example of the Abb type, suitable for measurements of refractive index to within 6 . 3 6.4 nearest t 0.1 OC. 6.5 the case of solid sample
34、s). Glass plate, of known refractive index. Water bath, thermostatically controlled, with a Water bath, capable of being maintained at the circulation pump, and capable of being maintained to the temperature at which the measurements are to be made (in 7 Sampling It is important that the laboratory
35、receive a sample which is truly representative and has not been damaged or changed during transportation or storage. Sampling is not part of the method specified in this International Standard. A recommended sampling method is given in IS0 5555. 8 Preparation of test sample Prepare the test sample i
36、n accordance with IS0 661. The refractive index shall be determined on dried and filtered fats and oils. In the case of a solid sample, transfer the sample prepared in accordance with IS0 661 to a suitable container and place it in the water bath (6.5), set at the temperature at which the measuremen
37、ts are to be made. Allow sufficient time for the temperature of the sample to stabilize. 9 Procedure NOTE accordance with 9.1 and 9.2. If it is required to check whether the repeatability requirement (1 1.2) is met, carry out two single determinations in 9.1 Calibration of the instrument Verify the
38、calibration of the refractometer (6.1) by measuring the refractive index of the glass plate (6.3) in accordance with the manufacturers instructions, or by measuring the refractive index of the ethyl laurate (5.1). 9.2 Determination Measure the refractive index of the test sample at one of the follow
39、ing temperatures: 2 Q IS0 2000 -All rights resewed Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- - STD=ISO b32
40、0-ENGt 2000 W Li651703 OBihLLB U19 IS0 6320:2000(E) a) 20 O C for fats and oils that are completely liquid at this temperature; b) 40 O C for fats and oils that are completely melted at this temperature but not at 20 OC; c) 50 O C for fats and oils that are completely melted at this temperature but
41、not at 40 OC; d) 60 “C for fats and oils that are completely melted at this temperature but not at 50 OC; e) 80 O C or above for other fats and oils, for example completely hardened fats or waxes. Maintain the temperature of the prism of the refractometer at the required constant value by circulatin
42、g through the instrument water from the water bath (6.4). Monitor the temperature of the water issuing from the refractometer using a suitable precision thermometer. Immediately before the measurement, lower the movable part of the prism to a horizontal position. Wipe the surface of the prism with a
43、 soft cloth and then with a piece of cotton wool moistened with a few drops of the solvent (5.2). Allow to dry. Carry out the measurement acccording to the operating instructions for the instrument being used. Read the refractive index to the nearest 0,000 1 as an absolute value, and record the temp
44、erature of the prism of the instrument. Immediately after the measurement, wipe the surface of the prism with a soft cloth and then with a piece of cotton wool moistened with a few drops of the solvent (5.2). Allow to dry. Measure the refractive index twice more, calculate the arithmetic mean o f th
45、e three measurements and take this as the test result. 1 O Calculation If the difference between the measurement temperature ti and the reference temperature t is less than 3 OC, the refractive index n; at the reference temperature t is given by the following equation: n;, = n; +(ti -t)F where tl is
46、 the measurement temperature, in degrees Celsius; t is the reference temperature (see 9.2), in degrees Celsius; F is the factor equal to 0,000 35 at t = 20 O C 0,000 36 at t = 40 OC, t = 50 O C and t = 60 O C 0,000 37 at t = 80 O C or above. If the difference between the measurement temperature tl a
47、nd the reference temperature t is 3 O C or more, the result should be discarded and a fresh determination made. Report the result rounded to the fourth decimal place. Q IS0 2000 -All rights reserved 3 Copyright International Organization for Standardization Provided by IHS under license with ISO Lic
48、ensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:07:55 MDTNo reproduction or networking permitted without license from IHS -,-,- - STD=ISQ b320-ENGL 2000 D 4853903 08LbLL9 T 5 5 D IS0 6320:2000(E) 11 Precision 11.1 Interlaboratory test Details of an interlaboratory test on the precision method are summarized in annex A. The values derived from this test may not be applicable to concentration ranges and matrices other than those given. 11.2 Repeatability The absolute difference between two indepen