《JIS-K-0115-2004-ENG.pdf》由会员分享，可在线阅读，更多相关《JIS-K-0115-2004-ENG.pdf（28页珍藏版）》请在三一文库上搜索。
1、J IS JAPANESE INDUSTRIAL STA N DA R D Translated and Published by Japanese Standards Association (JAIMA/ JSA) General rules for molecular absorptiometric analysis - ICs 71.040.50 Reference number : JIS K 0115 : 2004 (E) PROTECTED BY COPYRIGHT 15 S Copyright Japanese Standards Association Provided by
2、 IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- K 0115 : 2004 Foreword This translation has been made based on the original Japanese Industrial Standard revis
3、ed by the Minister of Economy, Trade and Industry through deliberations at the Japanese Industrial Standards Committee, as the result of proposal for revision of Japanese Industrial Standard submitted by Japan Analytical Instruments Manufacturers Association (JA1MA)I Japanese Standards Association (
4、JSA) with the draft being attached, based on the provision of Article 12 Clause 1 of the Industrial Standard- ization Law applicable to the case of revision by the provision of Article 14. Consequently JIS K 0115 : 1992 is replaced with this Standard. Attention is drawn to the possibility that some
5、parts of this Standard may conflict with a patent right, application for a patent after opening to the public, utility model right or application for registration of utility model after opening to the public which have technical properties. The relevant Minister and the Japanese Industrial Standards
6、 Committee are not responsible for identifying the patent right, application for a patent after opening to the public, utility model right or application for registration of utility model after opening to the public which have the said technical properties. Date of Establishment: 1964-11-01 Date of
7、Revision: 2004-03-20 Date of Public Notice in Official Gazette: 2004-03-22 Investigated by: Japanese Industrial Standards Committee Standards Board Technical Committee on Chemical Analysis JIS K O1 15 : 2004, First English edition published in 2004-08 Translated and published by: Japanese Standards
8、Association 4-1-24, Akasaka, Minato-ku, Tokyo, 107-8440 JAPAN In the event of any doubts arising as to the contents, the original JIS is to be the final authority. O JSA 2004 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by
9、any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. Printed in Japan PROTECTED BY COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not
10、 for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- Contents K 0115 : 2004 Page 1 2 3 4 4.1 4.2 4.3 4.4 5 5.1 5.2 5.3 5.4 5.5 6 6.1 6.2 6.3 7 7.1 7.2 8 8.1 8.2 8.3 8.4 Scope Normative references Definitions Apparatus Construction of apparatus .
11、Performance indication of apparatus . Accessory devices . Additional functions Operation method Conditions for apparatus setting up Calibration of wavelength graduation and absorbance graduation . Preparation of target sample Establishment of operational conditions for apparatus . Preparation for me
12、asurement . Measurement of absorption at specified wavelength Measurement by double beam method Measurement by single beam method . Measurement of cell blank value Measurement of absorption curve in the range of specified wavelength Measurement by double beam method Measurement by single beam method
13、 . Determination Working curve method Standard addition method . Other method for determination . Rate assay . 1 1 1 3 3 7 8 9 9 9 10 10 10 11 12 12 12 13 13 13 14 14 14 16 17 17 PROTECTED BY COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employ
14、ees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- K 0115 : 2004 8.5 9 9.1 9.2 10 11 Expression of determined value 17 Management of quality of data . 17 Confirmation of performance of apparatus . 17 Suitabl
15、e preparation of sample . 18 Recording of determined value . 18 Items to be described on each individual standard 18 Annex 1 (normative) Calibration method of absorbance graduations by optical filter 20 Annex 2 (normative) Calibration method of absorbance graduations by solution 21 (ii) PROTECTED BY
16、 COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- JAPANESE INDUSTRIAL STANDARD JIS K 0115 : 2004
17、General rules for molecular absorptiometric analysis 1 This Japanese Industrial Standard specifies general rules in the case where quantitative analysis is carried out by measuring the transmittance, absorbance or reflection within a range from near 200 nm to near 1 100 nm of wavelength by using a s
18、pectrophotometer or a photoelectric photometer. This Standard does not apply to the methods using an atomic absorption photometer, a near-infrared spectrophotometer or an infrared spectrophotometer. Scope 2 Normative references The following standards contain provisions which, through reference in t
19、his Standard, constitute provisions of this Standard. The most recent editions of the standards (including amendments) indicated below shall be applied. JIS K 0050 JIS K 0211 JIS K 0212 JIS K 0215 JIS K 0557 JIS K 8005 JIS K 8223 General rules for chemical analysis Technical terms for analytical che
20、mistry (General part) Technical terms for analytical chemistry (optical part) Technical terms for analytical chemistry (analytical instrument part) Water used for industrial water and wastewater analysis Reference materials for volumetric analysis Perchloric acid 3 Definitions 0211, JIS K 0212, JIS
21、K 0215 and the following definitions apply. For the purposes of this Standard, the definitions given in JIS K absorbance a numerical value obtained by taking common logarithms of a ratio of the intensity of the light which transmitted a sample to an intensity of the light before transmission transmi
22、ttance a ratio of an amount of the light after transmission to an amount of the light before transmission for expressing a proportion of the light which transmitted substance percent transmission, percentage transmission a value obtained by express- ing transmittance in percentage molar absorption c
23、oefficient, specific molar absorbance coefficient, molar absorptivity a coefficient obtained by converting absorbance of a specific sample into absorbance when a cell with a concentration of analyte of 1 mol& and op- tical path length of 1 cm is used specular reflection, regular reflection reflectio
24、n which follows a law of optical reflection like a mirror plane diffuse reflection reflection of which the light diffuses in many directions regardless of the laws of optical reflection PROTECTED BY COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license with JSALicensee=IHS
25、 Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- 2 K 0115 : 2004 percent reflection a value obtained by expressing a ratio of an intensity of reflected light from a target substance to an intensity
26、of reflected light from a reference substance for expressing a proportion of the light reflected on a surface of substance in percentage spectroscope 1) spectrograph, spectrometer an apparatus which makes a slit image on one focal plane in order of wavelength by making the light from one light sourc
27、e dispersed spectroscope apparatus in 3 h) 1) which is capable of making spectra which can be observed visually monochromator an instrument which isolates the light with a specified wave- length single monochromator a monochromator with a single dispersion element like a diffraction grating or a pri
28、sm double monochromator a monochromator, generally, composed of two single monochromators optically connected in series single beam an optical method in which an optical path does not branch be- tween the light source and the detector in a photoelectric photometer or a spec- tropho torne ter double
29、beam branch in the sample side and in the reference side optical filter a general term of optical devices used for passing or blocking the light with a specified range of wavelength gelatin filter an optical filter, made of coloured gelatin film laminated be- tween glass layers or the like, through
30、which the light with a specified range of wavelength is prepared interference filter an optical filter through which the light with required wave- length is prepared making use of interference of light on thin film or multi- layer film colour optical filter an optical filter, made of coloured glass,
31、 through which the light with a specified range of wavelength is isolated absorption cell a container which contains solutions, solvents, etc. in order to measure the absorption of light using them sample cell reference cell tions photometer an instrument which measures an intensity of light light s
32、ource a portion which makes a light wave (photon) generate and emit in a photometric analysis instrument 2) an optical method which makes the light from a light source an absorption cell which contains samples an absorption cell which contains solvents or reference solu- PROTECTED BY COPYRIGHT Copyr
33、ight Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- 3 K 0115 : 2004 phototube a photodetector which encloses the ca
34、thode which emits electrons by the exposure of light and the anode which receives electrons in the glass tube, and is not equipped with the intermediate electrode photomultiplier tube a photodetector which is equipped with the same pho- toelectron generating mechanism as that of a phototube, and fur
35、ther equipped with one or more intermediate electrodes which generate photoelectrons between the cathode and the anode in order to increase electrons photodiode a photodetector utilizing the photovoltaic effect produced by the exposure of the light to a P-N junction surface photodiode array a multic
36、hannel photodetector which arranges photodiodes one-dimensionally or two-dimensionally photo cell a photodetector utilizing the photovoltaic effect produced by the exposure of the light to a rectifying contact surface such as a contact surface of metal and semiconductor resolution capacity of a spec
37、troscope capable of separating two spectrum lines which are mutually adjacent dispersion dividing the light containing various wavelength components into the light for every wavelength component using a prism and/or a diffraction grating stray light light other than the light with an object waveleng
38、th in the light dispersed and taken out by the monochromator. white light analyte tion a continuous-spectrum light which looks white to the naked eye the target component to be analyzed in a sample or in a sample solu- 4 Apparatus 4.1 Construction of apparatus 4.1.1 Photoelectric photometer The exam
39、ple of construction of photoelectric pho- tometer is as shown in figure 1 and figure 2. The photoelectric photometer is com- posed of a light source part, wavelength selection part, sample part, photometry part, signal processing part, data processing part and indication, recording and output part.
40、The photoelectric photometer uses an optical filter as the wavelength selec- tion part. For the detection method, there is a method for detecting the light with a single wavelength and a method for detecting the light with multiple wavelengths. For the photometry system, there is a single beam metho
41、d and a double beam method. Furthermore, for the exposure system, there is a system of exposing the wavelength- selected light to a sample and a system of wavelength-selecting the light which trans- mitted a sample exposed to the white light. PROTECTED BY COPYRIGHT Copyright Japanese Standards Assoc
42、iation Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- 4 K 0115 : 2004 Wavelength selection part (optical filter) Light source ! ! I ! I processing
43、 part Data I Indication, recording , and output part Figure 1 Example of photoelectric photometer (exposing the wavelength-selected light to a sample) , , -! 1 - ! ! processing part Data I Indication, recording and output part Figure 2 Example of photoelectric photometer (wavelength- selecting the l
44、ight which transmitted a sample) a) Light source part sources, a power source for lighting, a light condensing system, etc. i) The light source part is composed of an emitter for light The emitter for light sources shall be as fol- Emitter for light sources lows: Tungsten lamp: used at a long wavele
45、ngth range of 320 nm or over Halogen lamp: used at a long wavelength range of 320 nm or over Deuterium lamp: used at a wavelength range from 160 nm to 400 nm Low pressure mercury lamp: many emission lines from 253.65 nm to 579.07 nm used as a light source Other light sources: A xenon lamp, a high lu
46、minance LED, a laser, etc. can be used. Power source for lighting This shall have a function to power a light source and to keep its luminance stable. Light condensing system This system is composed of a light condens- ing mirror and a light condensing lens. Wavelength selection part The wavelength
47、selection part is composed of a colour optical filter, a gelatin filter, an interference filter, or an optical filter with their combinations. When the monochromatic light source such as LED, laser beam is used, the wavelength selection part may be omitted. 2) 3) b) PROTECTED BY COPYRIGHT Copyright
48、Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 23:56:06 MDTNo reproduction or networking permitted without license from IHS -,-,- 5 K O115 : 2004 Sample part In the case of the double beam method, reference sample shall be fixed in optical path, and in beam method, either target sample or reference sample the target sample and the case of the single can be fixed in it, and is composed of an absorption cell and absorption-cell holder. 1) Abs