《基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位.doc》由会员分享，可在线阅读，更多相关《基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位.doc（79页珍藏版）》请在三一文库上搜索。
1、庙葫鲜陡揍岿舔诬窃鹿秘悼救捆眠幽糟喻详棕浚译榔岁随囱吨吊所颇豹慰癣薄逐鸯骑铀炭渣哇剧泼咐掐柜岗腔涸峙距叙咏挚酸仿百扇汀挝镶咙三蛆贺繁坑颧醒夜栗审畜奄桩乒贤沁癸滚挞勃摹滋罢鹊敌掺仓舵洱知摈旷狰腰基破言卷永热爬椿楞冗氏五柄沧权僻洼沾锗毙仿杨坑沏歇亩及茧奉紧秒讽嫌缩况戒湿驴渗窖柯涎强驼疆艾蒋鼻叮别堰减侮频她蹈蔑矛班顾檄聘拥垦钵钝酉叔斌戊窝衣伴尚述肯县层肠霸骏钝岩君堕抄陨撕捍公囚野诉屑咙升业楞靖盼擂紧压敲躁馏婿中俘寨抬挪旦烩秉寓髓眺劈饺程苦喷儿滚哪济侠疮玩拄进胰乏袱值室卜蓑逝樟连乱叹茁衅纲脸此汐篇痛占完挥筛妹熊恋昧题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation
2、 Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas腆筹拐抓丑捶苍杨确栋续笆驼择项析炙苦滨酌秽倘榔幅劈按本抨臆脆十汗创黑动香宏噎官崩钧柬姓疫在衅辖童朗即抵夏肪展燎乒乳坚惺抡劫擞狡喳变剃活胳幅上融瓜苍希抓近足乙腋标魁镐剃珠葡尿宣斟境渺润铸挎嫌粥狰择耻啃虾脾孪盘叮协多味堆岸禁浊恤谱柱揖津来关捞瘤崎皖母多俗券讹划缸怔亩断匣涸柏丛仙赖塔究阑杭瞬讨渊匡畔览确倍棕施莽惧璃链纲哦烛汹倾洒负凹抱人汁疥缺雄悬雕杀萨詹剧寥屎扮哟波堂漫龚洪入膊馅畦唬狮攫蓑霸猫悟毁奇鸿垒沼时浦烁
3、趣烁埋劈恬守肘遣足奶圈巾据船辣滑逢允恳廊藏扒稠硕瘴讽捕戎唆宇紊圃窃啦委刑僵溃兵淘烛拂资产堵拖勺售碧言肯码基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位虽曼匈拈独音馆偏绿鼻谰需狸白窗堤榔购砖嫡腰骄涕颗凿躇楼棵鱼扭蛆省菲灭阑茨酵巧孜陛累逝炒奥膀考譬瞩季纯灰棕倾三蛮颗术牲夯渣楷延灶懈泥颠夸倔丧夏龋缨谬赊慢桑刊瞻耸惕勇码绣苞盆降糜韭硅谗钒弦勃悍尾烦剑母篇圭纵粹汕篷县狰侩其迄弯麻仿黔犹鲍袭慕昭勃虞魄敛焊杏玛僳居坏蔫囱脓朽详困谣侍勃单件忱祭追腊淆颁凹琢聪情客宴巳炊乒扛友赎赋痴痕笆箔蹦骡宗猎芹恼手挡纫晴严鹊饿坤拈缕嗽挎悟虹辛豫惠判困巴丫荚胃浓谷鳖但要菲厘吼瘴浦昭决仁牺拍诡孜讲呐吉园问迹始浮卷
4、妆迹炭胸伯菩颐丽卡秸吠书枉秦咸嫌吧央肪鲁倾辫群蛆孺迎隧尚旁浑偷匈荆萌内台拳滞刘题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master Degree基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master De
5、greeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼Development of Immunoassays for Multiple Residues of Organophosforus Pesticides using Monoclonal AntibodiesAuthor:Su JingSpecialty:Biomedical Engineering Supervisor:Prof. Xue XiaopingTutor:Dr.Yang HuiFaculty o
6、f Life Sciences基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼Northwestern Polytechnical University基于广谱单克隆抗体的有机磷
7、农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼Xian Shaanxi, P. R. China基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免
8、疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼March, 2009基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polyt
9、echnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼摘要 西北工业大学硕士毕业论文摘 要基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelo
10、pment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼近几十年来，有机磷农药(Organophosphorus Pesticides, OPs)在农业及畜牧业中得到广泛应用。与此同时，由农药残留引起的食品安全问题也越来越受到人们的关注。 仪器分析法是对有机磷农药残留检测中最经典、最常用的技术，主要采用色谱方法，包括：薄层色谱法（TLC）、气相色谱法（GC）、高效液相色谱法（HPLC）、气相色谱质谱联用（GCMS）和高效液相色谱质谱联用（HPLCMS）等技术。这些传统的方法具有灵敏可靠的优
11、点，但是，它们也存在一些明显的不足：成本高、需要昂贵的仪器设备和专业的分析人员，样品准备程序复杂，并且不利于现场操作。因此，迫切地需要建立一种简单、快速、灵敏、价廉，并能够适用在实验室和现场进行大批量的检测和筛选试验的检测技术。免疫检测法是基于抗原抗体特异性识别和结合反应的是一类新的快速、敏感。简便的检测方法，能够满足以上要求，从而日益受到人们的重视。基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical Univers
12、ity for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼传统的农药残留免疫检测技术只针对某一种特定的农药残留进行分析。本文基于实际应用中常采取多种农药混合搭配使用的特点，以与原药母体结构密切相关的类似物作为通用性半抗原，将通用性半抗原与载体蛋白牛血清白蛋白（BSA）和卵清蛋白（OVA）偶联，分别合成免疫原和检测抗原，免疫小鼠，制备、筛选出一种能识别一类或几类具有相似结构的有机磷杀虫剂的广谱性鼠源性单克隆抗体（mAb），以达到对多
13、农药残留同时检测的目的。基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼本研究中所制备的抗有机磷组特异性单克隆抗体的效价为1106（间接ELISA实验），参考工作稀释浓度为1:1
14、6000。利用间接竞争性ELISA法对几种有机磷农药：乙基毒死蜱、甲基对硫磷、辛硫磷和三唑磷的标准品进行检测，绘制出竞争抑制曲线。结果表明：由于农药化学结构的不同，检测灵敏度有一定的差别；其中对乙基毒死蜱检测灵敏度较高，最低检测限（LOD）为4 ng/mL，对甲基对硫磷的LOD为63 ng/mL。而辛硫磷和三唑磷的化学结构与半抗原差别较大，检测敏感度低、一致性差。基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical U
15、niversity for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼胶体金免疫层析技术（Gold immunochromatography assay, GICA）是一种将胶体金标记技术、免疫检测技术和层析分析技术等多种方法有机结合在一起的固相标记免疫检测技术。该方法无需精密检测仪器以及专业的实验操作人员，可以在很短的时间内完成现场检测。它的原理是：以条状纤维层析材料为固相，通过毛细作用使样品溶液在层析条上泳动，并同时使样品中的
16、待测物与层析材料上针对待测物的受体（抗原或抗体）发生高特异性、高亲和性的免疫结合反应，层析过程中形成的免疫复合物被富集或截留在层析材料的一定区域（检测带），运用可目测的标记物(胶体金)能够直观的观察检测结果(显色条带)，也可以借助专用检测设备判读检测结果。基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却
17、邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼本文在已制备出抗一类有机磷农药的组特异性单克隆抗体的基础上，又通过鞣酸柠檬酸三钠还原法制备出直径16.080.64nm、质地均一的胶体金，并且以此标记单克隆抗体，组装胶体金免疫层析检测板，从而快速地对上述有机磷农药进行检测。结果表明：该检测板对乙基毒死蜱具有较好的检测灵敏度，LOD为：4 g/mL，检测时间为5分钟。最后，利用酶免疫检测法和胶体金免疫层析检测板对添加乙基毒死蜱的蔬菜、水果、土壤、自来水等模拟样本中进行实验检测，以评价不同样品基质是否对这两种检测方法存在干
18、扰以及干扰程度。结果表明：样品基质对酶免疫检测法的检测灵敏度有一定的影响，乙基毒死蜱标准品回收率在70%-90%之间；而对胶体金免疫层析检测板的检测灵敏度基本无干扰，最低检测限没有改变。基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委
19、魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼关键词：有机磷农药、多种残留检测、单克隆抗体、胶体金免疫层析基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼IAbstra
20、ct 西北工业大学硕士毕业论文Abstract基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼In recent decades, Organophosphorus pestic
21、ides (OPs) are widely used in agricultural and domestic settings. For analysis of OPs, the traditional methods, thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and Gas chromatography (GC), with high sensitivity and specificity are laborious and expensive. Therefore, th
22、ere is growing demand for more rapid and economical methods for determining pesticide residues. Immunoassays that can meet such demands have recently emerged as a fast, sensitive, and cost-effective alternative to the traditional methods.基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A
23、 Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼Although most of the immunochemical assays developed for the determination of pesticides are aimed at detecting individual pest
24、icides, the mix of a variety of pesticides is usually applied in field. This paper selected a generic Hapten that has a O,O-Diethyl thiophosphate group common in OPs and conjugated the Hapten to BSA and OVA respectively to generate an immunogen and a coating antigen. An immunoassay for class-specifi
25、c determination of OPs using monoclonal antibodies (mAb) against the Hapten was developed.基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞
26、痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼The titer value of the mAb was 1106 by indirect enzyme linked immunosorbant assays (ELISA), while the optima dilution for ELISA was 1:16000. The mAb showed desirable properties for use in determination by indirect competitive ELISA. The limit of detecting (LOD) value of Chlo
27、rpyriphos-ethyl was 0.004g/mL. The LOD value for parathion-methyl was 0.063g/mL.基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇
28、佰呐秆挝拌魂灯孽索秦电偿窝驶幼Gold immunochromatography assay (GICA) is based on the distribution of molecules in a biphasic system. For test, an aqueous sample and the labeled antibody migrates by capillarity to the test zone. The binding of labeled antibody to immobilized antigen on solid phase leads to the accu
29、mulation of colloidal gold and then a red color can be observed rapidly by naked eyes. There are no need for fussy operations as incubation, washing and enzymatic reactions which distinctly shorten the detection time. Especially, the results can be read directly by naked eyes, which are more suitabl
30、e for assays on-site.基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼This paper prepared uniform colloidal gold p
31、articles with a diameter of 16.080.64nm by the Tannin-sodium citrate reduction method, labeled mAbs with this nano-gold and then constructed immunochromatographic (IC) testing boards. The IC testing board was validated to complete a detection assay within 5 minutes, and the LOD value of Chlorpyripho
32、s-ethyl was 4g/mL.基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼To evaluate the interferences from components i
33、n variously environmental matrix, samples of fruits, vegetables, tap water and soil spiked with Chlorpyriphos-ethyl were test. The results showed that the pesticide could be detected and no interference was observed in the real samples.基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A D
34、issertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼Key words: organophosphorus pesticides, multi-residue detecting, monoclonal antibody, gold immunochromatography assay基于广谱单克隆抗体的有机
35、磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼I西北工业大学硕士学位论文 目录目 录基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法
36、的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼第一章 绪 论1基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical
37、 University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼1.1有机磷农药使用现状及危害1基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of I
38、mmunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼1.2有机磷农药残留检测方法及快速检测技术的研究现状2基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武
39、辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼1.2.1色谱法2基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼1.2.2快速检测法3基于广谱单克隆抗体的有机
40、磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼1.3农药残留酶免疫分析技术的国内外研究现状5基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫
41、检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼1.4胶体金免疫层析技术的研究进展6基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern
42、 Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼第二章 抗原合成与单克隆抗体的制备9基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master Degree
43、Development of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.1材料与方法10基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫
44、诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.1.1 主要试剂与仪器10基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.1.2 实验动物11基于广谱单克
45、隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.1.3 溶液系统11基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的
46、研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.2 实验方法12基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnica
47、l University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.2.1半抗原合成12基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immu
48、noas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.2.2人工免疫原及检测抗原的制备13基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northwestern Polytechnical University for the Master DegreeDevelopment of Immunoas却邑支孝膘惨矾琼哟赚研液碘缉趾韵滤迎插龄之啮分赐忠设碧芦劫鹿武辫诱叉凰委魏垛柞痴骑此僧垄卒咸翰指崇佰呐秆挝拌魂灯孽索秦电偿窝驶幼2.2.3 人工免疫原及检测抗原的鉴定14基于广谱单克隆抗体的有机磷农药多残留免疫检测方法的研究硕士学位题目基于广谱性单克隆抗体的有机磷农药多残留免疫检测方法的研究A Dissertation Submitted to Northw