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1、Food and Fermentation Industries()(,710062)(),(),:,:2004 -06 -2211. 1(clenbuteerol ,CLB) ,2 ()24223 , 52,C12 H18 Cl12 N2OHCl ,3131652,172,2605 min1 ,2 ,1020 min,23 h,5 h,2539 h ,5(97 %) ,58 d ,3 112( CLB)1964,CLB,2, 4 1984( > 1g/ kg,CLB510d) CLB,52080,CLB10 %15 %,8%15 %,CLBCLB,(cAMP),( FFA),;,CLB
2、,Mg2 +,622111082004 Vol.30 No.9 (Total 201),CLBCLB510,(3)(2539h),6,CLB,CLB107CLB,:2090,315,6 ,7 1987,;,8 ;, CLB,;,;K+Ca2 +Na +Mg2 +,T, ST,CLB,CLB,19971997 3CLB( MRL )( FDA)( WHO):012g/ kg016g/ kg016g/ kg012g/ kg0105g/ kg;MRL015g/ kg ,MRL015g/ kg8212,2716 mg1216 mg2,CLB45 mg/kg ,018g/ kg,100 gCLBCLB1
3、0,980, 10 ,11 CLB,WTO,CLB,( GMP)(HACCP)( IKB),CLB12 3,CLB,1092004309(201)HPLCCLB,GC2MS,CLBHPLC; HPLC,115 ng/ g ,GC2MS;,18 GC2MSHPLC, GC2MS,HPLCCE,pH,19 (TLC) 4 ,12 ,1517 GC2MS)3,F TIR )2GC(CE)R2biopharm2( HPLC2MS)2CLBEL ISA,CLB1. 2001 400,311 13 ,14 ,(115 cm) ,CLB ;,CLB312,CLB( HPLC)2313( IA)21,:110
4、2004 Vol.30 No.9 (Total 201),-zepto (10 -20 )yocto (10 24 ),20 ,CLB( RIA)( EIA)RIACLB,CLB ,214 ng/ g ,2123 EIACLB( EL ISA),Randox Laboratories LtdCLBEL ISA,EL ISA,2,24 EL ISA;,314CLB,25 ,CLB,243nm296 nmCLB,CLB15 ,CLB26 ,DIGFACLB,CLB13,.J .,2003 ,24 (5) :12714.J .,2003 ,4 :3132CLB,4CLBCLB,CLB( DIGFA
5、)(biosensor ,BS)2DIGFA,1,140J 1,2002 ,4 (9) :1 0351 0362111J 1,2002 ,15 (4) :43431J 1,2002 ,9 (5) :2802814,1J 1,2002 ,19 (6) :135Elliott C T , Mccaughey W J , Crooks S R H etal1Residues of clenbuterol in cattle receiving therapeu2 tic doses:Implicationsfor differentiating between legaland illegal us
6、eJ 1 Vet Q ,1995 ,17 :1001026,1J 1,2003 ,9 (4) :87887Sauern M J ,Pickett R J H ,Limer S et al1Distributionand elimination of clenbuterol in tissues and fluids ofcalves following prolonged oral administration at agrow -promoting doseJ 1J Vet Pharmacol Therap ,1995 ,18 :818681J 1,2002 ,7 :30319,1J 1,2
7、003 ,6 :1920101J 1,2002 ,21 (4) :293011,1J 1,2002 ,14 (5) :101112,.J .,2003 ,3 :282915,.J .,2002 ,12 (5) :63463616,.()J .,2003 ,10 :272817,.J .,2002 ,12 (2) :19319418Miyazaki T , Nakajima T , Hashimoto T et al. Deter2 mination of clenbuterol in animal tissues by high per2 formanceliquidchromatograph
8、yJ.J FoodHygSo2 ciety of Japan , 1995 ,36 (2) :26927319Giancarlo B , Robert A , Pietro T et al. Positivechemical ionization and tandem mass spectrometricfragmentation for the gaschromatographic analysis of2agonists using the ion trap technique J . JMassSpectrum ,1999 ,34 :1 3461 35320,.()J .,2002 ,1
9、9 (4) :323421Polettini A J , Segara G, Gonzalez X et al. Clen2 buterol and- adrenergic drugs detected in hair oftreated animals by ELISA J . Clin Chem , 1995 ,41 :94594622Sawya W N , Lone K, Husain A et al. Screening for2 Agonists in sheep urine and eyes by an enzyme2linkedimmunosorbent assay in the
10、 state of Kuwait J . FoodControl , 2000 ,11 :15(119)1112004309(201)3( POV)mmol/ kg/d0 48013 %01062 01068 01287013 %01062 01089 01301013 %01062 01132 01413013 %EDTA 01062 01066 012793,:75 %,:11M1:,2002. 4865022,1M1:,1998Study on Extraction Method and AntiSong ChangchunBao Qingsheng(Department of Basi
11、c Courses , Anhui Technical Teachers College , Bengbu , 233100) 2oxidation Propertyof Flavonoid Compound from Leaves of Diospyros kakiABSTRACTThe extraction method of flavonoid in leaves of Diospyros kaki was studied1 The opti2 mum extracting process was put forward , and anti2oxidation property of
12、the extract from the leaves ofDiospyros kaki was studied1 The results showed that the optimum extracting technical process was75 % alcohol1 Effect of flavonoid from the leaves of Diospyros kaki was higher than ascorbic acid andcitric acid1 Key wordsleaves of Diospyros kaki , flavonoid ,extraction method ,antioxidant(111) M.:,1995. 62923,.630J .,2003 ,19 (4) :42