《缓冲液配方表.docx》由会员分享，可在线阅读，更多相关《缓冲液配方表.docx（6页珍藏版）》请在三一文库上搜索。
1、25C下0.1mol/l磷酸钠缓冲液的配制pH1mol/l Na2HPO4(ml)1mol/l NaH 2PO4(ml)5.87.992.16.012.088.06.217.882.26.425.574.56.635.264.86.846.353.77.057.742.37.268.431.67.477.422.67.684.515.57.889.610.48.093.26.80.2mol/L磷酸盐缓冲液(pH5.78.0)pH0.2mol/L NaH 2PO«ml)0.2mol/L Na2HPO«ml)5.793.56.55.892.08.05.990.010.06.08
2、7.712.36.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.516.084.07.613.087.07.710.589.57.88.591.57.97.093.08.05.394.7NaH2PO4?2H2O : 10.86105g (或 NaHPO4?12H2O : 21.8502g ) + NaH2PO4?2H2O : 6.085
3、95g (或 NaH2PO4 ?H2O: 10.76g )，溶解，定容至1L上述药品都是极易溶的，直接溶解就行了附磷酸氢二钠 磷酸二氢钠缓冲液（0.2mol/L )pH值；0.2mol/LNa2HPO4溶液（mL ）;0.2mol/LNaH2PO4溶液（mL）5.8 ;8.092.05.9 ；10.090.06.0;12.387.76.1 ；15.085.06.2 ；18.581.56.3 ；22.577.56.4 ；26.573.56.5 ；31.5 ;68.56.6 ；37.562.56.7 ；43.556.56.8 ；49.051.06.9;55.045.07.0 ；61.039.07.
4、167.033.07.272.028.07.377.023.07.481.019.07.584.016.07.687.013.07.789.510.57.891.58.57.993.57.58.094.75.3NaH2PO4?2H2O:Mr=178.05,0.2mol/L溶液为35.61g/L;NaHPO4?12H2O:Mr=358.22,0.2mol/L溶液为71.64g/L;NaH2PO4?2H2O:Mr=156.03,0.2mol/L溶液为31.21g/L;NaH2PO4?H2O : Mr=138.01 , 0.2mol/L 溶液为 27.6g/L磷酸缓冲液(phosphate buff
5、er )按照下表所给定的体积，混合1 mol/L的磷酸二氢钠（单碱）和 1mol/L磷酸氢二钠（双碱）贮液，获得所需pH的磷酸缓冲液。配制1 mol/L的磷酸二氢钠（NaH2PO4"H2OT液：溶解138g于足量水中，使终体积为1L;1mol/L 磷酸氢二钠（Na2HPO4贮液：溶解142g于足量水中使终体积为 1L。1mol/L磷酸二氢钠（ml）1mol/L 磷酸氢二钠（ml）最终pH值8778508157757356856255655104503903302801231501852252653153754354905506106707206.06.16.26.36.46.56.6
6、6.76.86.97.07.17.21mol/L磷酸二氢钠（ml）1mol/L磷酸氢二钠（ml）最终pH值8771236.08501506.18151856.27752256.37352656.46853156.56253756.65654356.75104906.84505506.93906107.03306707.12807207.2Ph0.2mol/l NaH2PO4(ml)0.2mol/l Na2HPO4(ml)5.793.56.55.892.08.05.990.010.06.087.712.36.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.516.084.07.613.087.07.710.589.57.88.591.57.97.093.08.05.394.7