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1、CASE:1443371,F,64Y，体检发现肝脏肿物半月余。 症状、体征（-） 既往：肝段血管瘤 辅检：LAB肿瘤女-6：(2014-08-01),癌胚抗原CEA：5.36ng/ml(0-5ng/ml)；肝酶指标（-） 乙肝表面抗原：117 IU/ml 乙肝核心抗体IgG：13.36 COI,腹部B超:左肝外叶低弱回声结节，建议超声造影； 胃镜：慢性轻度浅表性炎，灶性肠化，幽门螺杆菌：阴性 肠镜：（-）,eWorldView Image,eWorldView Image,CT:左肝II段边缘可疑稍低密度灶，请结合其它检查或复查。 MR:左肝外侧叶占位，恶性病灶不能排除，转移？或其他。,腹腔镜
2、左肝肿块切除+术中冰冻 术中未见明显腹水，肝脏无明显结节性改变，左肝外侧叶可见一大小约2cm肿块，略突出于肝脏表面，质软，边界尚清，剖开肿瘤，切面灰黄色，送术中冰冻提示：淋巴细胞增生，淋巴瘤待排。,冰冻送检肝组织一块， 3.8*3*1.5cm，剖面见一灰白结节，1.7*1.5cm，镜示结节内淋巴组织增生，淋巴滤泡形成，生发中心可见，大小不等，局部伴小血管增生，结节周围围绕以不连续的纤维组织包膜，包膜局部见淋巴组织浸润，周围肝组织汇管区内亦可见成片淋巴细胞浸润。免疫组化（B片）：CD3 & CD20 & CD5 & CD10 & CD43 & CD15 & CD21 & CD23 & CD30
3、& Bcl2 & Bcl6 & Kappa & Lambda & Ki67：未见异常表达，EBER：，D240：内皮细胞+。 BCR：IgH基因重排：Fr2A+Fr2B：阴性；Fr3A：阴性；IgK基因重排：阴性。 （肝）淋巴组织反应性增生，考虑为假性淋巴瘤,background,Pseudolymphoma(PL) was first described in the lung by Salt-zstein in 1963 as a lymphocytic tumor associated with inflammation and with no evidence of systemic
4、dissemination. PL of the liver was first reported by Snover et al in 1981. PL can be found in the gastrointestinal tract,orbit,and pancreas,but rarely in the liver.,background,Hepatic pseudolymphoma (HPL),also termed as reactive lymphoid hyperplasia, or nodular lymphoid lesion. HPL has benign behavi
5、or. HPL is an extremely rare condition, with most reports to date dealing with single cases.,Etiopathogenesis,The exact etiology remains unknown. It is speculated that chronic infection or inflammatory process suggests their correlation with immunological response. HPL can develop in patients with a
6、utoimmune diseases, malignancy, or hepatitis or who are administered interferon therapy.,Based on a review of the PubMed database from 1981 to 2012 using the keywords “pseudolymphoma” and “lymphoid hyperplasia of the liver”, we found 64 lesions in 46 cases of pseudolymphoma.,Literature review,F/M: 4
7、2:5 Most of the cases (69.6%) were reported in Japan. The average size of the tumor was 15.1+/- 10.6 mm, most of the tumors (90.6%) were no more than 20 mm. Most of the lesions reported are single in number.,Imaging findings,On CT, a hypodense mass on unenhanced images that is slight enhanced on ear
8、ly-phase images after contrast injection and subsequent washout. On MRI, slight T1 and T2 prolongation and restricted diffusion.,Pathological features,HPL consisted of tumorous infiltrates of mature lymphocytes with multiple lymph follicles or clusters of epithelioid histiocytes. Lymphocytes charact
9、eristically extended into nearby portal tracts. No obvious atypical cells were identified. Ductal structures positive for cytokeratin 7 were entrapped in the peripheral parts of nodules. In situ hybridization of immunoglobulin light chains revealed B lymphocytes and plasma cells to be polyclonal. In
10、 addition, clonal rearrangements of immunoglobulin heavy chains could not be shown in any cases using PCR.,At the edge of the nodule, lymphocytic infiltration extends into perinodular portal tracts (left). Compared with the background liver with chronic hepatitis B (right), lymphocytic infiltration
11、is more pronounced in the portal tracts around the nodule,diagnosis,The diagnosis of hepatic pseudolymphoma can be challenging but might be aided by in situ hybridization,analyses of gene rearrangements, or follow-up based on images.,Treatment,Since clinical diagnosis is often difficult especially a
12、t its earlier stage, surgical resection appears a mainstay for diagnostic/therapeutic purpose.,CASE 1,F,49Y, The abdominal ultrasonography showed a hypoechoic lesion, about 20*16*14 mm in size, located in segment 2. hepatic enzymes(-); HBV surface antigen and HCV antibody were both negative AFP, CEA
13、, and CA-199 (-),(CT) scans showed a 20-mm-diameter, slightly hypodense mass with peripheral rim enhancement in arterial phase located in segment 2. (B) Early washout of the contrast medium with retained ring enhancement was seen in the portal phase. (C) Axial magnetic resonance (MR) imaging showed
14、a hypointense nodule on segment 2 of the liver in the T1-weighted image, and (D) the lesion became hyperintense in the T2-weighted image. (E) Arterial portography with computed tomography demonstrated a perfusion defect in segment 2 of the liver. Angiography showed a hypervascular lesion on segment
15、6 of the liver (arrow). (F) However, the small tumor in segment 2 of the liver on previous CTand MR had no definite tumor stain.,A hypovascular tumor was impressed,and peripheral type cholangiocarcinoma, metastatic tumor, or sclerosed hemangioma should be differentiated. A left lateral sectionectomy
16、 was performed.,Figure 2 Pathological findings of the lesion. Gross pathologic specimen revealed a well-defined, nonencapsulated, yellowish-white, and soft hepatic tumor, located just beneath the capsule in segment 2 of the liver.,Histopathology of hepatic pseudolymphoma. Hematoxylineeosin-stained h
17、istological images showed that the mass was composed of hyperplastic lymphoid tissue with several enlarged, irregularly shaped, well-demarcated follicles with formation of germinal centers distributed evenly in the mass. (A) The lymphocytes containing round nuclei with scant cytoplasm are mainly sma
18、ll in size and mature in appearance with scattered medium and large cells, ?40. Immunohistochemical stain showed that the germinal centers were composed of B lymphocytes (B) positive to CD20 antibody, ?100, (C) but negative to Bcl-2 (e) antibody, ?100. (D) The interfollicular area was composed of sm
19、all T lymphocytes positive to CD3 antibody, ?100. (E) Reactive immunoblasts positive to CD30 antibody were evenly distributed in the interfollicular region, ?100. (F) However, the EpsteineBarr virus-encoded RNA stain was negative, ?40.,CD20 (+), B cells,Bcl-2 (-) B cells,CD3 (+) small T cells,Reacti
20、ve CD30 (+) immunoblasts,EpsteineBarr virus- encoded RNA(-),The patient received regular follow-up, and no recurrence was found during 5 years of follow-up.,CASE 2,F,63Y, had a history of PBC and resection of the left adrenal gland for primary aldosteronism. She was asymptomatic on admission and her
21、 condition was generally good. Abdominal ultrasonography showed a hypoechoic lesion,10 mm in diameter in segment 7 . Laboratory tests(-),A: A hypodense nodule in plane phase; B: A hyperdense nodule in the early phase after injection of contrast medium; C: A hypodense nodule in the late phase after i
22、njection of contrast medium.,hypointense nodule on T1-weighted images; A hyperintense nodule on T2-weighted images; A hyperintense nodule in the early phase after injection of contrast medium; A hypointense nodule in the late phase after injection of contrast medium.,Superparamagnetic iron oxide-enh
23、anced MRI showing hyperintense nodules.A: 10 mm nodule in segment 7; B: 4 mm nodule in segment 6. CT during arterial portography showing hypointense nodules. A: 10 mm nodule in segment 7; B: 4 mm nodule in segment 6.,Imaging findings suggested HCC, although no other hypervascular tumor could be excl
24、uded. A right posterior segmentectomy was performed.,Macroscopically, the lesion in segment 7 was white and hard with clear margins.,Microscopically, the lesion consisted of a nodular lymphoid infiltrate with germinal centers,Macroscopically, the lesion in segment 7 was white and hard with clear mar
25、gins. Microscopically,the lesion consisted of a nodular lymphoid infiltrate with germinal centers In situ hybridization revealed no significant difference between the numbers of cells positive for kappa-chain and lambda-chain,CD3(+) T-cells,CD20(+) B-cells,Stained for light chains,Stained for light
26、chains,The patient had an uneventful postoperative course and has shown no sign of recurrence for 11 M.,Summary,HPL should be considered in the differential diagnosis of small hepatic tumors (20 mm), especially when a single hypovascular tumor is found in a female patient who associated with either autoimmune disease or chronic liver disease,and the patient has no risk factors of hepatocellular carcinoma.,eWorldView Image,谢谢！,