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1、a Date: 6 November 2007 Origin: International Latest date for receipt of comments: 29 February 2008 Project no.: 2007/02910 Responsible committee: AW/9 Microbiology Interested committees: Title: Draft BS ISO 17604:2003/AMD 1 Microbiology of food and animal feeding stuffs - Carcass sampling for mi cr
2、obiological analysis Supersession information: If this document is published as a standard, the UK implementation of it will supersede NONE and partially supersede. NONE If you are aware of a current national standard which may be affected, please notify the secretary (contact details below). WARNIN
3、G: THIS IS A DRAFT AND MUST NOT BE REGARDED OR USED AS A BRITISH STANDARD. THIS DRAFT IS NOT CURRENT BEYOND 29 February 2008. This draft is issued to allow comments from interested parties; all comments will be given consideration prior to publication. No acknowledgement will normally be sent. See o
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5、ssemination by e-mail within such an organization by committee members. Further copies of this draft may be purchased from BSI Customer Services, Tel: +44(0) 20 8996 9001 or email ordersbsi-. British, International and foreign standards are also available from BSI Customer Services. Information on t
6、he co-operating organizations represented on the committees referenced above may be obtained from the responsible committee secretary. Cross-references The British Standards which implement International or European publications referred to in this draft may be found via the British Standards Online
7、 Service on the BSI web site http:/www.bsi-. Direct tel: 020 8996 7445 Responsible Committee Secretary: K Seyde (BSI) E-mail: keith.seydebsi- Draft for Public Comment Head Office 389 Chiswick High Road London W4 4AL Telephone: +44(0)20 8996 9000 Fax: +44(0)20 8996 7001 Form 36 Version 7.0 DPC: 07/30
8、173371 DC Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI b Introduction This draft standard is based on international discussions in which the UK has taken an active part. Your comments on this draft are invit
9、ed and will assist in the preparation of the consequent standard. Comments submitted will be reviewed by the relevant BSI committee before sending the consensus UK vote and comments to the international secretariat, which will then decide appropriate action on the draft and the comments received. If
10、 no comments are received to the contrary, then the UK will approve this draft. If the international standard is approved, it is possible the text will be published as an identical British Standard. UK Vote Please indicate whether you consider the UK should submit a negative (with reasons) or positi
11、ve vote on this draft. Submission The guidance given below is intended to ensure that all comments receive efficient and appropriate attention by the responsible BSI committee. Annotated drafts are not acceptable and will be rejected. All comments must be submitted, preferably electronically, to the
12、 Responsible Committee Secretary at the address given on the front cover. Comments should be compatible with Version 6.0 or Version 97 of Microsoft Word for Windows, if possible; otherwise comments in ASCII text format are acceptable. Any comments not submitted electronically should still adhere to
13、these format requirements. All comments submitted should be presented as given in the example below. Further information on submitting comments and how to obtain a blank electronic version of a comment form are available from the BSI web site at: http:/www.bsi- Template for comments and secretariat
14、observations Date: xx/xx/200x Document: ISO/DIS xxxxx 1 2 (3) 4 5 (6) (7) MB Clause No./ Subclause No./ Annex (e.g. 3.1) Paragraph/ Figure/Table/ Note (e.g. Table 1) Type of com- ment Comment (justification for change) by the MB Proposed change by the MB Secretariat observations on each comment subm
15、itted 3.1 Definition 1 ed Definition is ambiguous and needs clarifying. Amend to read . so that the mains connector to which no connection . 6.4 Paragraph 2 te The use of the UV photometer as an alternative cannot be supported as serious problems have been encountered in its use in the UK. Delete re
16、ference to UV photometer. Microsoft and MS-DOS are registered trademarks, and Windows is a trademark of Microsoft Corporation. Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI THIS DOCUMENT IS A DRAFT CIRCULATED
17、 FOR COMMENT AND APPROVAL. IT IS THEREFORE SUBJECT TO CHANGE AND MAY NOT BE REFERRED TO AS AN INTERNATIONAL STANDARD UNTIL PUBLISHED AS SUCH. IN ADDITION TO THEIR EVALUATION AS BEING ACCEPTABLE FOR INDUSTRIAL, TECHNOLOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT INTERNATIONAL STANDARDS MAY ON OCCASION
18、 HAVE TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL TO BECOME STANDARDS TO WHICH REFERENCE MAY BE MADE IN NATIONAL REGULATIONS. RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT, WITH THEIR COMMENTS, NOTIFICATION OF ANY RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE AND TO PROVIDE SUPPORTING DOCUMEN
19、TATION. DRAFT AMENDMENT ISO 17604:2003/DAmd 1 International Organization for Standardization, 2007 INTERNATIONAL ORGANIZATION FOR STANDARDIZATION ORGANISATION INTERNATIONALE DE NORMALISATION ISO/TC 34/SC 9 Voting begins on: 2007-10-26 Secretariat: AFNOR Voting terminates on: 2008-03-26 Microbiology
20、of food and animal feeding stuffs Carcass sampling for microbiological analysis AMENDMENT 1 Microbiologie des aliments Prlvement dchantillons sur des carcasses en vue de leur analyse microbiologique AMENDEMENT 1 ICS 07.100.30 To expedite distribution, this document is circulated as received from the
21、 committee secretariat. ISO Central Secretariat work of editing and text composition will be undertaken at publication stage. Pour acclrer la distribution, le prsent document est distribu tel quil est parvenu du secrtariat du comit. Le travail de rdaction et de composition de texte sera effectu au S
22、ecrtariat central de lISO au stade de publication. Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI ISO 17604:2003/DAmd 1 ii ISO 2007 All rights reserved PDF disclaimer This PDF file may contain embedded typefac
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27、thout prior written permission being secured. Requests for permission to reproduce should be addressed to either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail co
28、pyrightiso.org Web www.iso.org Reproduction may be subject to royalty payments or a licensing agreement. Violators may be prosecuted. Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI ISO 17604:2003/DAM 1 ISO 200
29、7 All rights reserved iii Forword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested i
30、n a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commis
31、sion (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical
32、 committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall
33、 not be held responsible for identifying any or all such patent rights. Amendment 1 to ISO 17604:2003 was prepared by Technical Committee ISO/TC 34, Food stuffs, Subcommittee SC 9, Microbioligy. Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00
34、2007, Uncontrolled Copy, (c) BSI Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI ISO 17604:2003/DAM 1 ISO 2007 All rights reserved 1 Page 5, replace the first phrase of the article 1 “Scope” : “This Internation
35、al Standard specifies sampling methods for the detection and enumeration of microorganisms on the carcass surface of freshly slaughtered (red) meat animals.“ by : “This International Standard specifies sampling methods for the detection and enumeration of microorganisms on the carcass surface of fre
36、shly slaughtered meat animals.“ Page 5, at the end of the article 1 “Scope”, add : “Annex D specifies methods for the microbiological sampling of poultry carcasses .“ Add Annex D between page 13 “Table C.3 Evaluation of swabbing and excision methods“ and page 14 “Bibliography“. Annex D (normative) S
37、ampling of poultry carcasses D.1 Scope The International Standard ISO 17604 describes sampling methods for carcass surfaces of red meat animals. This annex specifies methods for the microbiological sampling of poultry carcasses. The sampling methods include 1) carcass rinsing; 2) skin sampling; 3) c
38、arcass swabbing. D.2 Normative references See 2 of ISO 17604 D.3 Sampling procedure The choice of the sampling method mainly depends on the aim of the microbiological examination, the sensitivity required and practical possibilities to carry out the sampling. Rinsing whole carcasses is a sensitive n
39、on-destructive method for the microbiological examination of poultry. Skin sampling is applied in relatively small areas of the carcasses ( 50 cm2 or 25 g). Skin sampling may be non-destructive (e.g., neck skin removal) or destructive (e.g., breast skin or tissue removal). Swabbing of poultry carcas
40、ses is a non- destructive method that can be applied to larger carcasses (e.g. turkeys). D.4 Sampling frequency See 4 of ISO 17604. Licensed Copy: London South Bank University, London South Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI ISO 17604:2003/DAM 1 2 ISO 200
41、7 All rights reserved D.5 Sampling points See 5 of ISO 17604. Poultry carcasses are usually sampled in the slaughterhouse after the inside-outside-washer and chilling, before further processing, such as freezing, cutting or packaging. D.6 Sampling sites See 6 of ISO 17604. D.7 Sampling techniques D.
42、7.1 Diluent and disinfectant D.7.1.1 Buffered peptone water (BPW) or another diluent depending on the microbiological examination to be performed D.7.1.2 Ethanol 70% (v/v) or alcohol wipes D.7.2 Materials D.7.2.1 Sterile gloves D.7.2.2 Stomacher-type bags (sterile), size dependent on sample size (ca
43、rcass or skin samples) D.7.2.3 Plastic tie wraps or equivalent (to secure the bag) D.7.2.4 Pair of scissors D.7.2.5 Sterile scalpels D.7.2.6 Sterile forceps D.7.2.7 Sterile square templates with internal area of, for example, 10 cm2 or 25 cm2. Licensed Copy: London South Bank University, London Sout
44、h Bank University, Fri Nov 16 07:15:41 GMT+00:00 2007, Uncontrolled Copy, (c) BSI ISO 17604:2003/DAM 1 ISO 2007 All rights reserved 3 D.7.3 Carcass rinsing Put on sterile gloves (D.7.2.1). Open a large stomacher-type bag (D.7.2.2) without touching the sterile interior of the bag. Grip the bag at the
45、 bottom seam and fold it back over your hand inside out. Using the hand with the bag reversed over it grab the bird from the slaughter line into the bag. Use the other hand to pull the bag back over the hand that grabbed the bird. Take care not to contaminate the exposed interior of the bag. Allow a
46、ny excess fluid to drain before reversing the bag back over the bird. Rest the bottom of the bag on a flat surface. While still holding the top of the bag slightly open, add the sterile diluent (D.7.1.1), usually in a quantity of 400 ml, to the bag containing the carcass, pouring the solution into t
47、he carcass cavity and over the exterior of the carcass. Expel most of the air from the bag, and then close the top of the bag. While securely holding the bag, rinse the bird inside and out using a rocking motion for approximately one minute. This is done by holding the bird through the bottom of the
48、 bag with one hand and the closed top of the bag with the other hand. Hold the bird securely and rock it in an arcing motion, alternating the weight of the bird from one hand to the other, ensuring that all surfaces (interior and exterior of the carcass) are rinsed. Rest the bag with the bird on a f
49、lat surface and, while supporting the bird, open the bag. With a gloved hand, remove the carcass from the bag, first letting any excess fluid drain back in to the bag. Take care not to touch the interior of the bag with your gloved hand. Secure the top of the bag so that the rinse fluid will not spill out or become contaminated. Alternatively aseptically transfer the rinse fluid from the bag to a