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1、BRITISH STANDARD BS 1982-3: 1990 Fungal resistance of panel products made of or containing materials of organic origin Part 3: Methods for determination of resistance to mould or mildew IMPORTANT NOTE. It is essential that this Part of BS 1982 is read in conjunction with Part 0, which is published s
2、eparately. Licensed Copy: sheffieldun sheffieldun, na, Fri Nov 24 08:16:03 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 1982-3:1990 This British Standard, having been prepared under the direction of the Wood Preservation Standards Policy Committee, was published under the authority of the Board of
3、BSI and comes into effect on 31 October 1990 BSI 08-1999 First published as BS 1982 June 1953 Second edition September 1968 Third edition as BS 1982-3 October 1990 The following BSI references relate to the work on this standard: Committee reference WPC/10 Draft for comment 87/52053 DC ISBN 0 580 18
4、441 2 Committees responsible for this British Standard The preparation of this British Standard was entrusted by the Wood Preservation Standards Policy Committee (WPC/-) to Technical Committee WPC/10, upon which the following bodies were represented: Association of Consulting Scientists British Pest
5、 Control Association British Wood Preserving and Damp-proofing Association Chemical Industries Association Department of the Environment (Building Research Establishment) Timber Research and Development Association Amendments issued since publication Amd. No.DateComments Licensed Copy: sheffieldun s
6、heffieldun, na, Fri Nov 24 08:16:03 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 1982-3:1990 BSI 08-1999i Contents Page Committees responsibleInside front cover Forewordii 1Scope1 2Principle1 3Materials and reagents1 4Apparatus and facilities2 5Sampling2 6Test specimens2 7Procedure2 8Assessment3 9V
7、alidity of the test3 10Test report3 Appendix A Example of test report including table of results4 Publications referred toInside back cover Licensed Copy: sheffieldun sheffieldun, na, Fri Nov 24 08:16:03 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 1982-3:1990 ii BSI 08-1999 Foreword This Part of B
8、S 1982 has been prepared under the direction of the Wood Preservation Standards Policy Committee. BS 1982 was published in 1968 as a single standard including three methods of test. This revision provides a fuller consideration of the possible hazards to organic based panel products and has been div
9、ided into Parts to allow each method to be kept up-to-date separately. The following Parts supersede BS 1982:1968 which is withdrawn. Part 0: Guide to methods for determination; Part 1: Method for determination of resistance to wood-rotting Basidiomycetes; Part 2: Method for determination of resista
10、nce to cellulose-decomposing microfungi; Part 3: Methods for determination of resistance to mould or mildew. In this Part, two additional test fungi have been added to the corresponding method in the 1968 edition. Two different test procedures are now used: Method L is similar to the test in the 196
11、8 edition in which the sample is held over a humidifying medium at high relative humidity and Method H, in which the sample is held directly in contact with an agar medium. The extent of attack is now assessed by a scale rating based on the percentage coverage of the sample with mould growth. WARNIN
12、G. This standard calls for the use of substances and procedures that may be injurious to health if adequate precautions are not taken. It refers only to technical suitability and does not absolve the user from legal obligations relating to health and safety at any stage. The procedures described in
13、this standard are intended to be carried out by qualified or other suitably trained and/or supervised personnel. Attention is also drawn to the comments on health and safety in Part 0 of this standard and to the caution in clause 2 of this Part. Technical Committee 38 Durability of wood and wood-bas
14、ed products of the European Committee for Standardization (CEN) has just commenced work, under a mandate from the Commission of the European Economic Community (EEC), on the classification of biological hazards and durability of timber, performance of treated timber, and the performance testing of p
15、reservatives. With the publication of European Standards arising from this work, this Part of BS 1982 will be amended, revised or withdrawn so as to remove any conflicting aspects. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards a
16、re responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages 1 to 4, an inside back cover and a back cover. This standar
17、d has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Licensed Copy: sheffieldun sheffieldun, na, Fri Nov 24 08:16:03 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 1982-3:1990 BSI 08-19991 1 Scope Th
18、is Part of BS 1982 describes two methods of test for the determination of the resistance of panel products to the growth of surface mould or mildew. The methods are applicable in particular to building materials required to present a decorative finish including particleboard, plywood and plasterboar
19、d. Method L is applicable to situations where the material is exposed to high humidity conditions but without wetting from liquid water (low moisture) and method H is applicable to situations where the material is exposed to direct wetting (high moisture) for prolonged periods. The tests described a
20、re also applicable to products that have received a preservative treatment. NOTEThe titles of the publications referred to in this standard are listed on the inside back cover. 2 Principle Test pieces are inoculated with spores of moulds that commonly attack manufactured building materials and are p
21、laced in dishes either over a humidifying medium (method L) or in direct contact with an agar medium (method H). The test pieces are then incubated at a temperature and humidity optimum for the growth of moulds and subsequently examined visually for the presence of mould growth. CAUTION. The test pr
22、ocedures involve handling and working with micro-organisms. It is important that personnel trained in microbiology should perform those parts of the test involving handling organisms and infected test specimens. It is essential that such personnel are familiar with the general recommendations on per
23、sonnel safety given in BS 2011-2.2J, in particular National appendix Z, and have appropriate equipment and facilities available. 3 Materials and reagents 3.1 Biological materials 3.1.1 Test fungi 3.1.1.1 Obligatory test fungi. The following fungi shall be included in every test. 3.1.1.2 Optional tes
24、t fungi. If other mould species are relevant to specific circumstances, these shall be obtained from a recognized culture collection. They shall be used in a parallel test. NOTEPure cultures recently isolated from naturally infected material are also relevant. These should be identified and deposite
25、d in a recognized culture collection. 3.1.1.3 Maintenance of strains. The strains shall be maintained and treated in accordance with the instructions of their collection of origin. Cultures shall be used for preparing the spore suspensions when they are between 14 and 28 days old, preferably between
26、 14 and 21 days old. NOTECultures less than 21 days old may also be used after having been stored, securely stoppered, in a refrigerator at a temperature of 6 3 C for up to 3 months. Cultures from previously unopened containers shall be used to make each batch of suspension and the stoppers shall no
27、t be removed until the spore suspension is about to be made. 3.1.2 Reference materials (optional). These shall be panel products or other materials of established performance in practice (see 8.3 of BS 1982-0:1990). 3.2 Other materials and reagents Water complying with grade 3 of BS 3978 shall be us
28、ed throughout. 3.2.1 Humidifying substrate. An hydrated, laminar, aluminium-iron-magnesium silicate1), exfoliated to yield particles up to 3 mm diameter, with a bulk density of 80 kg/m3 to 90 kg/m3. Particles less than 1 mm in diameter shall be removed by sieving. 3.2.2 Culture medium. The culture m
29、edium shall consist of a mineral salt agar with the following composition in distilled or deionized water. SpeciesStrain no.a Aspergillus versicolorIMI 45554 (Vuillemin) Tiroboschi Chaetomium globosumIMI 45550 Kunze ex Steudel Cladosporium cladosporioidesIMI 178517 (Fresenius) de Vries Paecilomyces
30、variotiiIMI 108007 Bainer Penicillium pinophilumIMI 114933 Hedgecock Stachybotrys atraIMI 82021 Corda Trichoderma virideIMI 45553 Persoon ex S F Gray a The acronym IMI refers to strains held by CAB International Mycological Institute (CMI), Ferry Lane, Kew, Surrey. 1) Vermiculite is suitable. Sodium
31、 nitrate (NaNO3)3.0 g/L Potassium dihydrogen1.0 g/L orthophosphate (KH2PO4) Magnesium sulphate heptahydrate0.25 g/L (MgSO4.7H2O) Potassium chloride (KCl)0.25 g/L Agar powder10.0 g/L Licensed Copy: sheffieldun sheffieldun, na, Fri Nov 24 08:16:03 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 1982-3:1
32、990 2 BSI 08-1999 NOTEMedia having this composition are available from commercial suppliers. 3.2.3 Wetting agent solution. Water containing 0.5 g/L of a wetting agent based on dioctyl sodium sulphosuccinate shall be used. 4 Apparatus and facilities Ordinary laboratory apparatus and in particular the
33、 following are required. 4.1 Culture chamber. A closed but not airtight vessel with a domed lid which allows air circulation but avoids droplets of condensation falling on the test samples. NOTEA 500 mm 350 mm seed propagator with a transparent plastics lid, or a large desiccator with the tap open a
34、re both suitable. 4.2 Culture vessels. Sterile 90 mm plastics or glass petri dishes without lids. 4.3 Incubation chamber. Incubator or room, dark and capable of being controlled at 24 1 C and 70 5 % r.h. 4.4 Microscope. Equipped for magnification of 10 to 50. 4.5 Hand lens. Magnification 10. 5 Sampl
35、ing A minimum of three replicate sheets of the panel product under test shall be sampled. Ensure that they are clean and as free as possible from contaminants that might otherwise support growth and give misleading results. 6 Test specimens 6.1 Sample specimens Reject from each sheet of the panel pr
36、oduct under test (clause 5) the 300 mm nearest to each edge. Cut a sufficient number of test specimens, 40 mm 40 mm, from each sheet using the whole thickness of the product, to provide two test specimens from each sheet for exposure using each test method. Reject any specimens that show defects suc
37、h as gaps, knot voids, veneer rupture, or discontinuous adhesion. 6.2 Control specimens A 40 mm disc of filter paper2) shall be included in every test to check the virulence of the spore suspension. 6.3 Reference materials (optional) Reference specimens shall be cut from reference materials as descr
38、ibed in 6.1. 7 Procedure 7.1 Method L: low moisture test conditions 7.1.1 Preparation of culture chamber Mix a suitable quantity of the humidifying substrate (3.2.1) with 3 to 4 times its mass of water (avoiding excess free water) and autoclave at 115 C for 30 min. Place the prepared medium in the c
39、ulture chamber (4.1) to give a depth of 10 mm. 7.1.2 Installation of test and control specimens Place the specimens in sterile petri dishes (4.2) and place the dishes without lids on the humidifying substrate in the culture chamber. Close the culture chamber and place it in the incubation chamber (4
40、.3) for 12 h prior to inoculation. 7.1.3 Preparation of spore suspension Gently add 10 mL of the wetting agent solution (3.2.3) to a prepared subculture of each test strain. Sterilize a platinum or a nichrome wire by heating to red heat in a flame and allowing to cool. Use this wire to gently scrape
41、 the surface of the culture to liberate spores. Agitate the liquid slightly to disperse the spores without detaching mycelial fragments. Gently decant the spore suspensions from all the test fungi into a flask containing a few glass beads (2 mm to 5 mm diameter). Mix the contents of the flask vigoro
42、usly to break up any lumps of spores. Filter the suspension through a sterile cotton or glass wool plug into a clean flask. Use the suspension on the day on which it is prepared and do not store it for future use. 7.1.4 Inoculation of test and control specimens Remove the lid from the culture chambe
43、r and evenly spray the face and the edges of each specimen with about 0.5 mL of the spore suspension (7.1.3) and replace the lid. 7.1.5 Incubation of test and control specimens Place the culture chamber containing the inoculated specimens in the incubation chamber (4.3) and incubate at 24 1 C for 4
44、weeks. Check the humidifying substrate at intervals and add water as necessary to compensate for any drying out that may have occurred. 7.2 Method H: High moisture test conditions 7.2.1 Preparation of culture chamber Use the procedure described in 7.1.1. 2) Whatman No. 1 filter paper or similar is s
45、uitable. Licensed Copy: sheffieldun sheffieldun, na, Fri Nov 24 08:16:03 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 1982-3:1990 BSI 08-19993 7.2.2 Preparation of culture vessels Prepare the mineral salt agar medium (3.2.2) and pour the prepared medium into the petri dishes under aseptic condition
46、s to give a layer 3 mm to 4 mm in depth. 7.2.3 Installation of test and control specimens Place the specimens on the agar medium in the petri dishes, and place the dishes without lids on the humidifying substrate in the culture chamber. Close the culture chamber and place it in the incubation chambe
47、r for 12 h prior to inoculation. 7.2.4 Preparation of spore suspension Use the procedure described in 7.1.3. 7.2.5 Inoculation of test and control specimens Use the procedure described in 7.1.4. 7.2.6 Incubation of test and control specimens Place the culture chamber containing the inoculated specim
48、ens in the incubation chamber (4.3) and incubate at 24 1 C for 4 weeks. Check the humidifying substrate at intervals and add water as necessary to compensate for any drying out that may have occurred. 8 Assessment For either method, at the end of the 4 week incubation period remove the culture chamb
49、er from the incubation chamber and place it in a suitable safety cabinet or fume cupboard equipped with air extraction facilities. Open the chamber and remove the specimens. Examine the upper face and edges of each specimen using the hand lens (4.5) or the microscope (4.4) as necessary to recognize mould growth. Assess the extent of surface growth of mould and classify it in accordance with the following scale. Calculate the notional mean rating for all six replicate test specimens. 9 Validity of the test The results shall be accepted as valid pro