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1、BRITISH STANDARD Sensory analysis Methodology Triangle test ICS 67.240 ? BS EN ISO 4120:2007 Incorporating May 2009 corrigendum Licensed CopyChinese University of Hong Kong, 13/06/2009 08:05, Uncontrolled Copy, (c) BSI This British Standard was published under the authority of the Standards Policy a
2、nd Strategy Committee on 1 July 2004 BSI 2009 National foreword The UK participation in its preparation was entrusted to Technical Committee AW/12, Sensory analysis. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport t
3、o include all the necessary provisions of a contract. Users are responsible for its correct application. BS EN ISO 4120:2007 This British Standard is the UK implementation of EN ISO 4120:2007. It is identical with ISO 4120:2004. It supersedes BS ISO 4120:2004, which is withdrawn. Compliance with a B
4、ritish Standard cannot confer immunity from legal obligations. ISBN 978 0 580 59377 2 Date Comments 31 May 2009 This corrigendum renumbers BS ISO 4120:2004 as BS EN ISO 4120:2007 Amendments/corrigenda issued since publication Licensed CopyChinese University of Hong Kong, 13/06/2009 08:05, Uncontroll
5、ed Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 4120 July 2007 ICS 67.240 English Version Sensory analysis - Methodology - Triangle test (ISO 4120:2004) Analyse sensorielle - Mthodologie - Essai triangulaire (ISO 4120:2004) Sensorische Analyse - Prfverfahren - Dreiecksprfun
6、g (ISO 4120:2004) This European Standard was approved by CEN on 12 July 2007. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibl
7、iographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility
8、 of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ir
9、eland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassa
10、rt, 36 B-1050 Brussels 2007 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 4120:2007: E Licensed CopyChinese University of Hong Kong, 13/06/2009 08:05, Uncontrolled Copy, (c) BSI IS:0214 O4002(E) I SO 4002 All irthgs ersedevr i
11、ii Contents Page Foreword iv 1Scope 1 2Normative references . 1 3Terms and definitions. 1 4Principle . 2 5General test conditions and requirements. 3 6Assessors 3 6.1Qualification 3 6.2Number of assessors 3 7Procedure. 4 8Analysis and interpretation of results 4 8.1When testing for a difference 4 8.
12、2When testing for similarity. 5 9Test report 5 10Precision and bias 5 Annex A (normative) Tables 6 Annex B (informative) Examples. 10 Bibliography . 15 BS EN ISO 4120:2007 EN ISO 4120:2007 (E) Licensed CopyChinese University of Hong Kong, 13/06/2009 08:05, Uncontrolled Copy, (c) BSI Foreword The tex
13、t of ISO 4120:2004 has been prepared by Technical Committee ISO/TC 34 “Agricultural food products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 4120:2007 by Technical Committee CEN/SS C01 “Food Products“, the secretariat of which is held by CMC. This
14、European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by January 2008, and conflicting national standards shall be withdrawn at the latest by January 2008. According to the CEN/CENELEC Internal Regulations, the
15、 national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Nor
16、way, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 4120:2004 has been approved by CEN as EN ISO 4120:2007 without any modifications. iv BS EN ISO 4120:2007 EN ISO 4120:2007 (E) Licensed CopyChinese University of Hong
17、Kong, 13/06/2009 08:05, Uncontrolled Copy, (c) BSI INTENRATIONAL TSANDADR IS:0214 O4002(E) I SO 4002 All irthgs ersedevr 1 Sensory analysis Methodology Triangle test 1 Scope This International Standard describes a procedure for determining whether a perceptible sensory difference or similarity exist
18、s between samples of two products. The method is a forced-choice procedure. The method is applicable whether a difference exists in a single sensory attribute or in several attributes. The method is statistically more efficient than the duo-trio test (described in ISO 10399), but has limited use wit
19、h products that exhibit strong carryover and/or lingering flavours. The method is applicable even when the nature of the difference is unknown i.e. it determines neither the size nor the direction of difference between samples, nor is there any indication of the attribute(s) responsible for the diff
20、erence. The method is applicable only if the products are fairly homogeneous. The method is effective for a) determining that either a perceptible difference results (triangle testing for difference), or a perceptible difference does not result (triangle testing for similarity) when, for example, a
21、change is made in ingredients, processing, packaging, handling or storage; b) or for selecting, training and monitoring assessors. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. F
22、or undated references, the latest edition of the referenced document (including any amendments) applies. ISO 5492:1992, Sensory analysis Vocabulary ISO 8589:1988, Sensory analysis General guidance for the design of test rooms 3 Terms and definitions For the purposes of this document, the terms and d
23、efinitions given in ISO 5492 and the following apply. 3.1 alpha-risk -risk probability of concluding that a perceptible difference exists when one does not NOTE This is also known as Type I error, significance level or false positive rate. BS EN ISO 4120:2007 EN ISO 4120:2007 (E) Licensed CopyChines
24、e University of Hong Kong, 13/06/2009 08:05, Uncontrolled Copy, (c) BSI IS:0214 O4002(E) 2 I SO 4002 All irthgs ersedevr 3.2 beta-risk -risk probability of concluding that no perceptible difference exists when one does NOTE This is also known as Type II error or false negative rate. 3.3 difference s
25、ituation in which samples can be distinguished based on their sensory properties NOTE The proportion of assessments in which a perceptible difference is detected between the two products is given the symbol pd. 3.4 product material to be evaluated 3.5 sample unit of product prepared, presented and e
26、valuated in the test 3.6 sensitivity general term used to summarize the performance characteristics of the test NOTE In statistical terms, the sensitivity of the test is defined by the values of , and pd. 3.7 similarity situation in which any perceptible differences between the samples are so small
27、that the products can be used interchangeably 3.8 triad those three samples given to an assessor in the triangle test NOTE In the triangle test, each sample is marked with a different code. Two of the samples are alike (i.e. from one product) and one is different (i.e. from the other product). 4 Pri
28、nciple The number of assessors is chosen based on the sensitivity desired for the test. (See 6.2 and the discussion in A.3.) Assessors receive a set of three samples (i.e. a triad) and are informed that two of the samples are alike and that one is different. The assessors report which sample they be
29、lieve to be different, even if the selection is based only on a guess. The number of correct responses is counted and the significance is determined by reference to a statistical table. BS EN ISO 4120:2007 EN ISO 4120:2007 (E) Licensed CopyChinese University of Hong Kong, 13/06/2009 08:05, Uncontrol
30、led Copy, (c) BSI IS:0214 O4002(E) I SO 4002 All irthgs ersedevr 3 5 General test conditions and requirements 5.1 Clearly define the test objective in writing. 5.2 Carry out the test under conditions that prevent communication among assessors until all the evaluations have been completed, using faci
31、lities and booths that comply with ISO 8589. 5.3 Prepare the samples out of sight of the assessors and in an identical manner (i.e. same apparatus, same vessels, same quantities of product). 5.4 Assessors shall not be able to identify the samples from the way in which they are presented. For example
32、, in a taste test, avoid any differences in appearance. Mask any irrelevant colour differences using light filters and/or subdued illumination. 5.5 Code the vessels containing the samples in a uniform manner, preferably using three-digit numbers, chosen at random for each test. Each triad is compose
33、d of three samples, each with a different code. Preferably, different codes should be used for each assessor during a session. However, the same three codes may be used for all assessors within a test, provided that each code is used only once per assessor during a test session (e.g. if several tria
34、ngle tests on different products are being conducted in the same session). 5.6 The quantity or volume served shall be identical for the three samples in each triad, just as that of all the other samples in a series of tests on a given type of product. The quantity or volume to be evaluated may be im
35、posed. If it is not, the assessors should be told to take quantities or volumes that are always similar whatever the sample. 5.7 The temperature of the three samples in each triad shall be identical, just as that of all the other samples in a series of tests on a given type of product. It is prefera
36、ble to present the samples at the temperature at which the product is generally consumed. 5.8 The assessors shall be told whether or not they are to swallow the samples or whether they are free to do as they please. In this latter case, they shall be requested to proceed in the same manner for all t
37、he samples. 5.9 During the test sessions, avoid giving information about product identity, expected treatment effects, or individual performance until all testing is completed. 6 Assessors 6.1 Qualification All assessors should possess the same level of qualification, this level being chosen on the
38、basis of the test objective (see ISO 8586-1 and ISO 8586-2 for guidance). Experience and familiarity with the product may improve the performance of an assessor and, therefore, may increase the likelihood of finding a significant difference. Monitoring the performance of assessors over time may be u
39、seful for increased sensitivity. All assessors shall be familiar with the mechanics of the triangle test (i.e. format, task and evaluation procedure). 6.2 Number of assessors Choose the number of assessors so as to obtain the sensitivity required for the test (see the discussion in A.3). Using large
40、 numbers of assessors increases the likelihood of detecting small differences between the products. However, in practice, the number of assessors is often determined by material conditions (e.g. duration of the experiment, number of available assessors, quantity of product). When testing for a diffe
41、rence, typical numbers of assessors are between 24 and 30. When testing for no meaningful difference (i.e. similarity), twice as many assessors (i.e. approximately 60) are needed for equivalent sensitivity. BS EN ISO 4120:2007 EN ISO 4120:2007 (E) Licensed CopyChinese University of Hong Kong, 13/06/
42、2009 08:05, Uncontrolled Copy, (c) BSI IS:0214 O4002(E) 4 I SO 4002 All irthgs ersedevr Avoid replicate evaluations by the same assessor whenever possible. However, if replicate evaluations are needed to produce a sufficient number of total evaluations, every effort should be made to have each asses
43、sor perform the same number of replicate evaluations. For example, if only ten assessors are available, have each assessor evaluate three triads to obtain a total of 30 evaluations. NOTE Treating three evaluations performed by ten assessors as 30 independent evaluations is not valid when testing for
44、 similarity using Table A.2. However, the test for difference using Table A.1 is valid even when replicate evaluations are performed (see 9 and 10). Recent publications (see 6 and 7) on replicated discrimination tests suggest alternative approaches for analysing replicated evaluations in discriminat
45、ion tests. 7 Procedure 7.1 Prepare worksheets and scoresheets (see B.1 and B.2) in advance of the test so as to utilize an equal number of the six possible sequences of two products, A and B: ABB AAB ABA BAA BBA BAB Distribute these at random in groups of six among the assessors (i.e. use each seque
46、nce once among the first group of six assessors; use each sequence once again among the next group of six assessors, etc.). This will minimize the imbalance that results if the total number of assessors is not a multiple of six. 7.2 Present the three samples of each triad simultaneously if possible,
47、 following the same spatial arrangement for each assessor (e.g. on a line to be sampled always from left to right, in a triangle array). Within the triad, assessors are generally allowed to make repeated evaluations of each sample as desired (if, of course, the nature of the product allows for repea
48、ted evaluations). 7.3 Instruct the assessors to evaluate the samples in the order in which they were presented. Inform the assessors that two of the samples are the same and that one is different. Each assessor should then indicate which one of the three samples is different from the other two. 7.4
49、Each scoresheet should provide for a single triad of samples. If an assessor is to carry out more than one test in a session, collect the completed scoresheet and unused samples prior to serving the subsequent triad. The assessor shall not go back to any of the previous samples or change the verdict on any previous test. 7.5 Do not ask questions about preference, acceptance or degree of difference after the initial selection of the odd s