《BS-5711-18-1979.pdf》由会员分享，可在线阅读，更多相关《BS-5711-18-1979.pdf（8页珍藏版）》请在三一文库上搜索。
1、BRITISH STANDARD BS 5711-18: 1979 Methods of Sampling and test for glycerol Part 18: Detection of sugars NOTEIt is recommended that this Part of BS 5711 be read in conjunction with the general information given in BS 5711-0 “General introduction”, issued separately. UDC 661.188.1:547.426.1:543.064:5
2、47.455/.458 Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 This British Standard, having been prepared under the direction of the Chemicals Standards Committee, was published under the authority of the Executive Board and co
3、mes into effect on 30 November 1979 BSI 12-1999 The following BSI references relate to the work on this standard: Committee reference CIC/6 Draft for comment 75/53579 DC ISBN 0 580 10999 2 A British Standard does not purport to include all the necessary provisions of a contract. Users of British Sta
4、ndards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages 1 and 2, an inside back cover and a back cover. Thi
5、s standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Amendments issued since publication Amd. No.Date of issueComments Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00
6、:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 BSI 12-1999i Contents Page 1Scope and field of application1 2References1 3Principle1 4Reagent1 5Sampling1 6Procedure1 7Test report1 Publications referred toInside back cover Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00
7、 2006, Uncontrolled Copy, (c) BSI ii blank Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 BSI 12-19991 1 Scope and field of application This British Standard describes a colorimetric method for detecting the presence of suga
8、rs in glycerol. 2 References The titles of the publications referred to in this standard are listed on the inside back cover. 3 Principle Heating a test portion with a dilute sulphuric acid solution of urea and tin (II) chloride for 15 min on a steam bath. Development of a marked colour (usually bro
9、wn) indicates the presence of sugar. 4 Reagent The reagents used shall be of a recognized analytical grade. Distilled water complying with the requirements of BS 3978 or water of at least equal purity shall be used. Reagent solution. Dissolve 4 g of urea and 0.2 g of tin (II) chloride by heating wit
10、h 10 ml of approximately 400 g/l aqueous sulphuric acid. NOTEIt is usually convenient to make at least 30 ml of solution. 5 Sampling Prepare the laboratory sample as described in Part 2 of this British Standard. 6 Procedure Introduce 4 drops of the sample into each of two test tubes, 150 mm by 12.5
11、mm. To one tube add 1 ml of the reagent solution and to the other add 1 ml of distilled water, as a control (note 2). Immerse both tubes in a steam bath and examine after 15 min (note 3). If a marked colour develops in the tube containing the reagent, sugars are present. NOTE 1A marked colour shows
12、the presence of 0.25 % or more of sugars. For methods of determination of sugars, see clause 7 of Part 22 of this standard. NOTE 2If preferred, a reference mixture can be made of several crude glycerols known to be sugar-free. The control is prepared by using 4 drops of the reference mixture in the
13、second tube and following the procedure described in clause 6. NOTE 3The time chosen is sufficient to give a significant colour change even with low concentration of sugars, though in many cases 5 min would suffice to develop a colour. 7 Test report 7.1 Report the result as either “sugars detected”
14、or “no sugars detected”. 7.2 The test report shall include the following particulars: a) a reference to this British Standard; b) the result and the method of expression used; c) any unusual features noticed during the test; d) any operations not included in this British Standard or regarded as opti
15、onal. Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI 2 blank Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 BSI 12-1999 Publications referred to BS 3978, Water for
16、laboratory use. BS 5711, Methods of sampling and test for glycerol. BS 5711-2, Methods of sampling. BS 5711-22, Additional methods of test (non-specification). Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18: 1979 BSI 389 Chiswick
17、 High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by
18、amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions. It is the constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using this British Standa
19、rd would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front cover. Tel: 020 8996 9000. Fax: 020 8996 7400. BSI offers members an individual updating service called PLUS which ensures that subscribers automatically receive the latest ed
20、itions of standards. Buying standards Orders for all BSI, international and foreign standards publications should be addressed to Customer Services. Tel: 020 8996 9001. Fax: 020 8996 7001. In response to orders for international standards, it is BSI policy to supply the BSI implementation of those t
21、hat have been published as British Standards, unless otherwise requested. Information on standards BSI provides a wide range of information on national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services a
22、re also available which give details on all its products and services. Contact the Information Centre. Tel: 020 8996 7111. Fax: 020 8996 7048. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details
23、of these and other benefits contact Membership Administration. Tel: 020 8996 7002. Fax: 020 8996 7001. Copyright Copyright subsists in all BSI publications. BSI also holds the copyright, in the UK, of the publications of the international standardization bodies. Except as permitted under the Copyrig
24、ht, Designs and Patents Act 1988 no extract may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, photocopying, recording or otherwise without prior written permission from BSI. This does not preclude the free use, in the course of implementing the st
25、andard, of necessary details such as symbols, and size, type or grade designations. If these details are to be used for any other purpose than implementation then the prior written permission of BSI must be obtained. If permission is granted, the terms may include royalty payments or a licensing agreement. Details and advice can be obtained from the Copyright Manager. Tel: 020 8996 7070. Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI