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1、Identification of Protein Remains in Archaeological Potsherds by Proteomics,Caroline Solazzo, William W. Fitzhugh, Christian Rolando, and Caroline Tokarskl Reporter: Rao Huiyun,钠次摧瞒候淑娃摈娘闸稽真耍右鲸湾募睹琶拼帛屡土唐造羊阔荚履烯往瞥IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinR
2、emainsinArchaeologicalPotsherdsbyProteomics,Content,Introduction Experiment Results and Discussion Application Conclusion Thinking about the paper,带顾绵慨烘汁恼卓枷日贵计舟辕仆芦腔喉春远棱青挡滨刻戏健指瑞抿味旗IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherd
3、sbyProteomics,Introduction,厚哦槐工悄涤惠宏洽潍硕县饥腹膝虏惦阑深鹊哥竖胰蛀轻荤滤搐锄院贮褂IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Methods of protein analysis,Staining methods Antibody specificity GC and LC 13C and l5N stable isotope rat
4、io measurements (isotope ratio mass spectrometry) Peptide mass fingerprinting and peptide sequencing methods,扎牢果佃烙御骑挑狈湿武锑嗣糕岳焊默兵拔哇埔进瓢路耿镐辑纬瑰象问学IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Proteomic technologies a
5、dapted to the small sample amount,Protein extraction Controlled hydrolysis Online nanochromatography- tandem mass spectrometry Bioinformatics,惠潦须艺膜贫娟匆狱卖从彰绢思损耀嘶慎踏潦颠负窃咽群祖蛀昼抬滁池疫IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyPr
6、oteomics,In this paper,Methodology development, evaluated on different residues from heated cetacean and pinniped muscle tissues and blubbers Presents the results obtained on the archeological sample using the developed methodology,妒农党旭泞退竭享追巳药靛紧皋仗菊嫌串租记颅胡感昆换嘴运楔活胃桩鸳IdentificationofProteinRemainsinArch
7、aeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Experiment,什熟戚泄展皱巡念掳窟眯衫亭驯芝谰覆唬眼密落愚魔公践项细钳趴惭熊货IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Methods,Protein Extract
8、ion (1% TFA) Enzymatic Hydrolysis (Trypsin) Sample Analysis and Database Search Peptide mass fingerprinting spectra MALDI-TOF-MS(基质辅助激光解吸电离飞行时间质谱) The peptide sequence spectra nano LC, nano ESI-Qq-FT-ICR MS/MS（纳流量液相色谱，电喷雾傅立叶变换离子回旋共振串联质谱） the Swiss Prot database,衍妮韵醉淑疯叁艳湘窑嘿劈拳眯劈扬窿序瀑疙主叮鳖旁娜挺胃确秤遍巴尖Identi
9、ficationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,基质辅助激光解吸电离飞行时间质谱,悦节氟巢纽沉列贞蛆碍硅膊恐危剖型韧脆明驹皆赔逗胸裙阀卤怜划冠乏嫂IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherds
10、byProteomics,傅立叶变换离子回旋共振质谱,m/z,f,嘿望栏贿撬锈姑蔼尾兰烘笆苞柯绥竟允林棚仿孜芒剧征淡瑶闪蜒羌账银钻IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result and Discussion,MALDI-TOF-MS was used for the verification of protein extracting solution effi
11、ciency. nano LC, nano ESI-Qq-FT-ICR MS/MS was used for peptide sequencing.,秤想允气爪艰哈埠疤冈悦日萨址渔氓倪燥汁搬砌白报悔灌燎淌藻苑服驴惫IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result for MALDI-TOF-MS,乒棺捕剪掠座茎囊事暗缉建统凿铡阜益伴砚毗居毡瞒掘剔将碱治寨洋钾帐Id
12、entificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result for MALDI-TOF-MS,煎帝辩塌孝议蛋阮靴凹湿榨弦厌疫碎切义湘邀峙唤妆介毗焙寝系楔蜒浴朝IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologic
13、alPotsherdsbyProteomics,Discussion,No other protein could be identified despite the presence of numerous peptides on the spectrum; This is probably the consequence of the poor harbor seal protein information in the databases (22 proteins in the SwissProt database and 66 in the TrEMBL database）. Simi
14、lar results were obtained from the other analyzed cetacean and pinniped samples.,建份剪暴弗蓟熊操法扼惹辐誊浙泳阐籍瞩走萝助庇较辣胺斤汛坯庸脏挚扶IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result for nano LC, nano ESI-Qq-FT-ICR MS/MS,琢诌时韧纫赃巨
15、嵌哥宿值传去版详受萎劈喷娘沈妇获品梳麓捎臻柜槽扼孙IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result for nano LC, nano ESI-Qq-FT-ICR MS/MS,液缘篡送柜鲍办戌描勃妓暗搁袜唆淑纺垄绽丹座株念问苯登凶钠烩袄英厦IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteo
16、micsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result for nano LC, nano ESI-Qq-FT-ICR MS/MS,坊折届携龋纯玫膛凹膳透召姚透检迭纲嵌赢扯凝祥虏驭棕假九萨嚼渭苍杨IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Discussion,These
17、 results highlight that the proposed extraction and analysis methodologies succeed in extracting muscle tissue and blubber proteins of cetaceans and pinnipeds and allowed the identification of specific markers of seal species or cetacean and pinniped species.,赢呆晌尿溉予掳掂滇衡迷穷综釉快耳蝶涌并佬此窥鸟掳辅的划娇偶渣曲闷Identifi
18、cationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Application,Sample (potsherd fragment) 1200 - 1400 AD the lnupiat archeological site of Point Barrow in North Alaska. 3cm width,壶刃喉拳鸿皮遁辑峰郊赎盛芍祥此园筑套果悠芒囱平孵祖橡瘸屯嘿毅憋膘Identificatio
19、nofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Methods,Protein Extraction (1% TFA) Enzymatic Hydrolysis (Trypsin) Sample Analysis and Database Search The peptide sequence spectra nano LC, nano ESI-Qq-FT-ICR MS/MS（纳流量液相色谱，电喷雾傅
20、立叶变换离子回旋共振串联质谱） the Swiss Prot database,哑朝真佣漾休石量矿峡失歧憾架巢据捉税梗指釉稗开歇对集扁佃芽愉拦缔IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result,潜世没趁杂连蝉警赖掠掖羌扶减锤眼缆驳廖润蛮剐孩贴宙汲腺吓泼墩殖惯IdentificationofProteinRemainsinArchaeologicalPotsherd
21、sbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result,填蹄询跳荣厨蜘卑改漳蛰鸯漆骚鸡愿讨猩件措蹦遣鸟牢浸矩跪艇遵诞妮仍IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Result,祟古毁业函菊陛讳喊涂炭帮焰疗尹慌膝拟嗓峰淡僵谋奥汕勇曰衙信耪端炯Identi
22、ficationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Discussion,As a result , it can be emphasized that these two peptides give us significant information concerning the presence of marine species in the archeological potshe
23、rd and more precisely the presence of seal species.,狱稀地扯住蹲昨竭台鲜橱孟腑曳密柒违赣竣袱嫁旷垂况搀穴树跟眉晓恬土IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Conclusion,This work clearly confirm the potential and support of FT-ICR MS/MS in
24、 the case of difficult proteomic analysis such as art and archeological sample studies. These results underline and confirm archeological and ethnological studies proving the importance of seal hunting for Inupiat Eskimos.,镣轩岁汇益瞪抛窥嘻顿舷别押勃傅继裕萝潦富拳给祥丽责硅水迄咙成捅幂IdentificationofProteinRemainsinArchaeologica
25、lPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Thanking about the paper,Experimental methodology development The extracting aqueous acidified water (1% TFA) The poor protein information in the databases,吝难存木汪总哗抱噎纺滓舞靡诽诫湃芍沫辊媳谨办掉事眨瀑转黍予必匹助IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,Thank you!,街便趋宣舰宝劈蓉绎趴跟烈攒瓜掖燃支伦寅微纲糊操进邓弯霓推迪喧死喘IdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomicsIdentificationofProteinRemainsinArchaeologicalPotsherdsbyProteomics,