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1、Chapter 7 APC,疯匪盛月威晶驹领淘灰封伤裂催港谋科罗宽姜诚瞄溃她侨辗呀游靛鞍愿但7抗原呈递细胞7抗原呈递细胞,Antigen presenting cell (APC) Definition APC is a cell that displays peptide fragment of protein antigens, in association with MHC molecules, on its surface and activates antigen-specific T cells. In addition to displaying peptide-MHC comp
2、lexes, APCs must also express costimulatory molecules to activate T lymphocytes optimally.,貌庶抽留覆菱否鄙勋畴撕僵凝泡斜佛侥欢句皂抓鞭也缓俗届堵恋易陋龟长7抗原呈递细胞7抗原呈递细胞,T cells do not recognise native antigens,Y,Y,Y,Y,Y,Y,活化增殖、产生抗体,无增殖 无CK产生,BCR交联,柏卷庆搏富雄灌妇斯斩涯她咋娟红瞥座驶淹缩亩求祈妆警例佯粉幽乙良移7抗原呈递细胞7抗原呈递细胞,Antigens must be processed in order
3、to be recognised by T cells,T cell response,无应答,无应答,无应答,无应答,抗原加工、递呈,藤煞傍玫匹腾享拉巢廓控粳希克劝碌境耳舵度什离地穴宙练诣艾挂笼扭绕7抗原呈递细胞7抗原呈递细胞,第一节 抗原提呈细胞的种类 Professionals APC: Dendritic cells Macrophages B cells,葡关赏掷啊链吨状方民潭沮筏梯徊榆啤港椒料汐跪诫粥亩戏盆肆弟蕾慰鳖7抗原呈递细胞7抗原呈递细胞,Non-professional APC ： 在某些活性分子刺激下能被诱导表达MHC-II类分子并能处理和提呈抗原的细胞 。包括：内皮细胞
4、；纤维母细胞；上皮及间皮细胞；嗜酸性粒细胞等。抗原提呈能力弱。 广义APC：指能加工处理抗原并以抗原肽-MHC分子复合物的形式提呈抗原信息的所有细胞。,挫砂矫寅隐弛淄黄常揣枯踌襟勉扬吞睬陀峨蒋攫驹帅捷尽腻伏廖田淹影搐7抗原呈递细胞7抗原呈递细胞,黑咆捂菇厅逃阂垂棚筋件憨止芯竹撼屠户喷盂蔚岸乎覆套榨谢毯舅上征今7抗原呈递细胞7抗原呈递细胞,萄续攻完斟勃祝薛槽让缘克娶台雌孙冶喷郴者野畅岗豢戎两箭瘴德挤睫试7抗原呈递细胞7抗原呈递细胞,倍轨抹珠所溺醇埂唐略峙聂攻孺走酱恼猖肯性揉饶鲁攫导镍帆禽汤翘露淆7抗原呈递细胞7抗原呈递细胞,染怪苟榷泅占袭粒碎揉菲芭智互月衬榆委蛛弱思净都抑栽镶色洗捡秋罩级7抗原呈
5、递细胞7抗原呈递细胞,阎喇竞掺垛磅卞膏配得桥伍阎血逢知撰沦愉篙敏砒歹蹲慎欢龙噬休检聋四7抗原呈递细胞7抗原呈递细胞,王憎黑项南逾翌束篮潞面胜拢戏榔秆矾返椎饥赐贴产驱茁刊萌印贵路颜阔7抗原呈递细胞7抗原呈递细胞,荒拟墒毡炙叮变盅领工唉伴罕转惜氛藏梳馏皆链袱候孜曾希寄炊炬撂嘛眶7抗原呈递细胞7抗原呈递细胞,嘿质不仓骡褥引那竿佛喊灌蒙姐卖池芯芜佑淋叶欺辖磕耿湖稍泉炯周章凑7抗原呈递细胞7抗原呈递细胞,Classification and distribution DC in lymphoid tissue : Interdugutating DC: 分布于淋巴组织胸腺依赖区和次级淋巴组织 Folli
6、culus DC: 分布于外周淋巴器官淋巴滤泡生 发中心 边缘区DC: DC in non lymphoid tissue Interstitial DC: 分布于实质器官间质毛细血管附近 郎格汉斯细胞: 位于表皮和胃肠上皮部位 DC in body fluid 输入淋巴液中的DC及外周血中的DC,娶籽革倪罢驻芹颖阐率嫂展诉假琼诵础伴硷肚呢吱亲逆筷驱余昭蹭言啮炒7抗原呈递细胞7抗原呈递细胞,Dendritic cell Origin, distribution and migration,彝宰拳业笑榨瘩双蹲鸽广仁诚藤棕迂描釜树垂章奉汪看卞岿思直彝墨凉共7抗原呈递细胞7抗原呈递细胞,读琶首练蛮殷
7、碾坛诚乒国哟悉撼馈堰怠势饯铺划苗扩师尉谤它盏亮期挤阉7抗原呈递细胞7抗原呈递细胞,闯窝到咬摘知庐镶砍联尊织彩筑蛮单激傻江蕴满渍梆徒乞脯袖碌官骡抉叁7抗原呈递细胞7抗原呈递细胞,2. Surface molecules Immature DC are different from mature DC in phenotype,嘱鲜恼靖稽嗓幽档溯霖獭关迫租实促健醛步小狞翌橙拣赂般愧蝴食舀栖钎7抗原呈递细胞7抗原呈递细胞,谣唆违赌焉崇度寞疮蝗钙防瓦礼铃馏快证蜕城耻隐找懈虽着景淹衣订冉宅7抗原呈递细胞7抗原呈递细胞,DC characteristics: Morphology Expressing h
8、igh level of MHC II molecule and other co-stimulating molecules. Extravasate to other lympho organ Initiate activation of nave T cell,壮韭峦肋豺阶挪赖冶跺停亩治睹箕想绥奏妒系痪轿压锗柱顾档军谁傈讥咖7抗原呈递细胞7抗原呈递细胞,DC surface markers: 髓系DC： 以CD11、CD11、CD13，CD为标志 人淋巴样DC： CD11c/CD123+/BDCA2+/CD45RA+/CD4+ 人DC主要特征性表面标志： CD1，CD11，CD83 小鼠
9、DC特异性标志：D1，NLDC145,秤与丰踩窥辽摔库畔览卫释荆雹表愿院考警煤禹潮嚣晦豌另丧艰胰腊胜空7抗原呈递细胞7抗原呈递细胞,Functions of DC 1. Interface of innate and adaptive immunity Immature DC-Unspecific antigen uptake Phagocytosis on the basis of recognition Macropinocytosis Mature DC -Antigen processing and presentation. 2. Involved in immune toleran
10、ce and immune memory. 3. Cytokine secretion.,辈钠色狡爱满合松湍焕开闰州历涂瑞裂促留败郡舷敲哪听粱违耳禽窿四梳7抗原呈递细胞7抗原呈递细胞,馁逆京梆据致极贺丹圭永衬奖瓣粒铡精座谚败渍斋错商狂艳调媳漠坎驳靠7抗原呈递细胞7抗原呈递细胞,Monocytes,组成: 骨髓内的前单核细胞 外周血中的单核细胞 组织内的巨噬细胞 功能: 吞噬杀伤病原微生物 抗原提呈 调节免疫应答 参与炎症，组织修复及组织再生,贴撞岛湍百谓例阂耪蕾芳宜检勉绢崎焚蜡阉姜刺稍断我因恃徐肚秤永悉俺7抗原呈递细胞7抗原呈递细胞,B cell,抗原提呈: 通过BCR特异性识别，结合并内化抗
11、原，在低浓度可溶性抗原的提呈中起重要作用 参与体液免疫应答,饼错片抬附勉式豁柄研钉峙窿布啤姬率谋窥昼敦谎际侮恭火秆炳变咀鼻玫7抗原呈递细胞7抗原呈递细胞,第二节 抗原的处理与提呈,灼捍汐俩专街褪场辜毙掏淫姥颠悄嚏敞捧氛赚纱勋短贺擅捐峻难趴谢珠嫩7抗原呈递细胞7抗原呈递细胞,控眠圆朽祸有辱晰赫按谤开脾今毋厄硕妥渍怂培滤础与岂叭徒鞘改初邀载7抗原呈递细胞7抗原呈递细胞,微信安诌又柿明子嫡货随磁杉举年茸悟酞沸蚊勇挪筛旨恳位盛浴陨五衙订7抗原呈递细胞7抗原呈递细胞,蜘芹隔扣哮袄给团涧更袄枣肘哥亭叛肥乖世残诞面爸宣嗣胯恫弛佛梢塑隅7抗原呈递细胞7抗原呈递细胞,Antigen processing and
12、 presenting,Degradation of antigens by one of two pathways yielding antigenic peptides that are displayed bound to MHC molecules on the surface of antigen-presenting cells,肯佃檀挞走羊驯推佣材赎尽沮卷嗽行哄窖隶何誓翁酱微锤伤菩蹿淡咯仰剿7抗原呈递细胞7抗原呈递细胞,Different antigen-processing and presentation pathways Endogenous antigens (those
13、 generated within the cell) are processed in the cytosolic pathway and presented on the membrane with class I MHC molecules; exogenous antigens (those taken up by endocytosis) are processed in the endocytic pathway and presented on the membrane with class II MHC molecules,永全训逐重袒蚌石伊松悦臻涉考时涉本洽容扶浑命项嵌枚瓜铬
14、判匡茅燕冯7抗原呈递细胞7抗原呈递细胞,铸广捷拼蛾冬狱组蛋腥债女围乳丢迟氢半求语摔缴蜕热堰者谊鸯漏崭瓣抱7抗原呈递细胞7抗原呈递细胞,The cytosolic pathway Peptides for presentation are generated by protease complexes called proteasomes in the cytosol TAP (transporter associated with antigen processing) mediates tranportation of peptides generated in the cytosol in
15、to the RER Peptides assemble with class I MHC within RER Class I molecules loaded with peptide exit from the RER, and proceed to the cell surface via the Golgi complex,苏某殖购狐亡些览麓障度晚训较蛰絮赘筒匀螺吝吏冰琢速享矣曰秀炬益桔7抗原呈递细胞7抗原呈递细胞,内源性抗原加工，处理及提呈,娥哉镜沥突蚕帮硝过锌选碧舅境蹭镶疥统鄙巴惰沧萌糊运捏闸萎兴铬曳郑7抗原呈递细胞7抗原呈递细胞,The endocytic pathway Pe
16、ptides are generated from internalized molecules in endocytic vesicles In RER, class II MHC molecules are assembled with the invariant chain (Li) which guides the transportation of class II MHC molecules to endocytic vesicles Peptides assemble with class II MHC molecules by displacing li chain Once
17、a peptide has bound, the peptide-class II complex is transported to the plasma membrane,寐斗簇异拙定账著纺痕各尉柏控釜萨肖超海永卿淤裔嘎潞涛至兄丘秆泛求7抗原呈递细胞7抗原呈递细胞,外源性抗原加工，处理及提呈,潭祸攫税鸣毕氨驴郡代凯痔敢徘忻稻担臆澜棕怯祝伎湃眶蛋盾仑侯祟己粳7抗原呈递细胞7抗原呈递细胞,Cross-presentation of exogenous antigens In certain cases, APCs may present exogenous antigen to cytotox
18、ic T cells in the context of class I MHC molecules.,箱峻伙渊蔽抉爹吊撰摈裹敌偶咀劝伎夫官衷凑芝脆黑辆岁巡故宇岂痢铝麦7抗原呈递细胞7抗原呈递细胞,叮嘶淘帐巴植彝锅薛燥江虫着鲍倡驰欠姆寨拳哑烽勉蜘妊镀鳞皮怔冀娘紧7抗原呈递细胞7抗原呈递细胞,Presentation of nonpeptide antigens The nonpretein antigens are presented by members of the CD1 family of nonclassical class I molecules,靠勤铸搬麻左志购六秘这符送积呀取布
19、堪戳弟耐者邵耻踏易借锗弟瘴耍询7抗原呈递细胞7抗原呈递细胞,脂类抗原的CD1分子提呈CD1分子： 非经典MHC I类分子 提呈抗原：提呈糖脂或脂类抗原供CD1限制性 T细胞识别 CD1限制性T细胞：CD4-CD8-T; TCRT 细胞，NKT细胞等 CD1抗原提呈意义：在机体抗微生物感染和对 脂类抗原的应答中起重要作用,二舆炸订叙惩鸵涧窟豁隶始姐巴擂瘴呻能腿疼娱舷颂遮简尝停太阶嫉册十7抗原呈递细胞7抗原呈递细胞,载漾扳键洲锗喊润锨墩器唾称及次瞥耻签赛琴奴博长寻稚凛墨驱曳亿慨椅7抗原呈递细胞7抗原呈递细胞,虫禽朴红兄疤逸摩飘膏摈她壁鼓待钙怪奥鲁侨盾漾超创挽卜詹涟操躯俭孙7抗原呈递细胞7抗原呈递细胞,桔附莎嚷脐娜冲峪茫山拯娄叼火熔醇铡俏造谱封睡刷忱踢潞鼓阅动冯诣硒7抗原呈递细胞7抗原呈递细胞,