《BS-EN-ISO-14593-2005 BS-6068-5.23-1999.pdf》由会员分享，可在线阅读，更多相关《BS-EN-ISO-14593-2005 BS-6068-5.23-1999.pdf（22页珍藏版）》请在三一文库上搜索。
1、BRITISH STANDARD BS EN ISO 14593:2005 BS 6068-5.23: 1999 Incorporating Amendment No. 1 which renumbers BS 6068-5.23:1999 as BS EN ISO 14593:2005 also numbered as BS 6068-5.23:2005 Water quality Part 5: Biological methods Section 5.23: Evaluation of ultimate aerobic biodegradability of organic compou
2、nds in aqueous medium Method by analysis of released inorganic carbon in sealed vessels (CO2 headspace test) The European Standard EN ISO 14593:2005, with the incorporation of amendment A1:2005, has the status of a British Standard ICS 13.060.70 ? Licensed Copy: sheffieldun sheffieldun, na, Sat Nov
3、18 02:00:10 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN ISO 14593:2005 This British Standard, having been prepared under the direction of the Health and Environment Sector Committee, was published under the authority of the Standards Committee and comes into effect on 15 June 1999 BSI 11 Novemb
4、er 2005 ISBN 0 580 32464 8 National foreword This British Standard is the official English language version of EN ISO 14593:2005. It is identical with ISO 14593:1999. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/5, Biological
5、methods, which has the responsibility to: aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promul
6、gate them in the UK. A list of organizations represented on this subcommittee can be obtained on request to its secretary. This is one of a series of standards on water quality, others of which have been, or will be, published as sections of BS 6068. The various sections of BS 6068 are comprised wit
7、hin Parts 1 to 7, which, together with Part 0, are listed below. Part 0: Introduction; Part 1: Glossary; Part 2: Physical, chemical and biochemical methods; Part 3: Radiological methods; Part 4: Microbiological methods; Part 5: Biological methods; Part 6: Sampling; Part 7: Precision and accuracy. NO
8、TE The tests described in this British Standard should only be carried out by suitably qualified persons with an appropriate level of biological expertise. Standard biological procedures should be followed throughout. Cross-references The British Standards which implement international publications
9、referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary p
10、rovisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, the EN title page, the EN foreword page, the ISO ti
11、tle page, pages ii and iii, a blank page, pages 1 to 13 and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Amendments issued since publication Amd. No.DateComments 15
12、78011 November 2005Renumbers ISO 14593:1999 as BS EN ISO 14593:2005 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 14593 June 2005 ICS 13.060.70 English version Water quality - Evalua
13、tion of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test) (ISO 14593:1999) Qualit de leau - Evaluation en milieu aqueux de la biodgradabilit arobie ultime des composs organiques - Mthode par analys
14、e du carbone inorganique dans des rcipients hermtiquement clos (Essai au CO2 dans lespace de tte) (ISO 14593:1999) Wasserbeschaffenheit - Bestimmung der vollstndigen biologischen Abbaubarkeit organischer Substanzen im wssrigen Medium - Verfahren mittels Bestimmung des anorganischen Kohlenstoffs in g
15、eschlossenen Flaschen (CO2-Headspace-Test) (ISO 14593:1999) This European Standard was approved by CEN on 25 May 2005. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without
16、any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made
17、by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, G
18、reece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Cen
19、tre: rue de Stassart, 36 B-1050 Brussels 2005 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 14593:2005: E Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:00 2006, Uncontrolled Copy, (c) BSI Foreword The
20、text of ISO 14593:1999 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 14593:2005 by Technical Committee CEN/TC 230 “Water analysis“, the secretariat of which is held by DIN. This Europe
21、an Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2005, and conflicting national standards shall be withdrawn at the latest by December 2005. According to the CEN/CENELEC Internal Regulations, the nat
22、ional standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, P
23、ortugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 14593:1999 has been approved by CEN as EN ISO 14593:2005 without any modifications. EN ISO 14593:2005 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:00 2006, Uncontr
24、olled Copy, (c) BSI EN ISO 14593:1999 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 14593:1999 ii BSI 11 November 2005 Contents Page Forewordiii 1Scope1 2Normative reference1 3Terms and definitions1 4Principle3 5Test environment3 6R
25、eagents3 7Apparatus4 8Procedure4 9Calculation and expression of results8 10Expression of results9 11Validity of results9 12Precision of the method10 13Test report10 Annex A (informative) Example of a biodegradation curve11 Annex B (informative) Statistical treatment of results11 Annex C (informative
26、) Interlaboratory ring test12 Bibliography13 Figure A.1 Biodegradation of octan-1-ol in the CO2 headspace test11 Table 1 Final distribution of test and reference compounds in the test vessels7 Table C.1 Results of a ring test12 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:0
27、0 2006, Uncontrolled Copy, (c) BSI EN ISO 14593:1999 BSI 11 November 2005 iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through IS
28、O technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates c
29、losely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulat
30、ed to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 14593 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. Annex A, Annex B
31、 and Annex C of this International Standard are for information only. Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:00 2006, Uncontrolled Copy, (c) BSI blank Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:10 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 145
32、93:1999 BSI 11 November 2005 1 WARNING Activated sludge and sewage may contain potentially pathogenic organisms and should be handled with appropriate precautions. Hazardous test compounds and those whose properties are unknown should be handled with care. 1 Scope This International Standard specifi
33、es a method, by analysis of inorganic carbon, for the evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic substances at a given concentration of microorganisms. This International Standard is applicable to organic compounds which are: a) water-soluble under the test c
34、onditions; b) poorly water-soluble under the test conditions, in which case special measures may be necessary to achieve a good dispersion of the compound (see ISO 10634); c) volatile; d) not inhibitory to the test microorganisms at the concentration chosen for the test. In this test, biogenically p
35、roduced inorganic carbon is measured in situ in the test vessels in such a manner that the rate measured nearly equals the rate of microbial production. NOTE 1 The conditions described in this International Standard do not always correspond to the optimal conditions for allowing the maximum degree o
36、f biodegradation to occur. For alternative biodegradation methods see ISO 15462. NOTE 2 With highly volatile substances, losses to the gaseous phase can be minimized by reducing the volume of the headspace. However, there should be sufficient oxygen in the test system to prevent biodegradation being
37、 oxygen-limited. NOTE 3 The presence of an inhibitory effect can be determined as specified in 8.3, or by using any other method for determining the inhibitory effect of a substance on bacteria (see, for example, ISO 8192). 2 Normative reference The following normative document contains provisions w
38、hich, through reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the p
39、ossibility of applying the most recent edition of the normative document indicated below. For undated reference, the latest edition of the normative document referred to applies. Members of IEC and ISO maintain registers of currently valid International Standards. ISO 10634:1995, Water quality Guida
40、nce for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in aqueous medium. 3 Terms and definitions For the purposes of this International Standard, the following terms and definitions apply. 3.1 ultimate aerobic biodegra
41、dation breakdown of a chemical compound or organic matter by microorganisms in the presence of oxygen to carbon dioxide, water and mineral salts of any other elements present (mineralization) and the production of new biomass 3.2 primary biodegradation structural change (transformation) of a chemica
42、l compound by microorganisms resulting in the loss of a specific property 3.3 activated sludge biomass produced in the aerobic treatment of wastewater by the growth of bacteria and other microorganisms in the presence of dissolved oxygen Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 02:00:1
43、0 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 14593:1999 2 BSI 11 November 2005 3.4 concentration of suspended solids amount of solids obtained by filtration or centrifugation of a known volume of activated sludge and drying at about 105 C to constant mass 3.5 total organic carbon TOC all that
44、 carbon present in organic matter which is dissolved and suspended in the water sample 3.6 dissolved organic carbon DOC that part of the organic carbon in the water sample which cannot be removed by specified phase separation NOTE For example, by centrifugation at 40 000 m s2 for 15 min or by membra
45、ne-filtration using membranes with pores of diameter 0,2 4m to 0,45 4m. 3.7 total inorganic carbon TIC all that inorganic carbon in the water deriving from carbon dioxide and carbonate 3.8 dissolved inorganic carbon DIC that part of the inorganic carbon in water which cannot be removed by specified
46、phase separation NOTE For example, by centrifugation at 40 000 m s2 for 15 min or by membrane-filtration using membranes with pores of diameter 0,2 4m to 0,45 4m. 3.9 theoretical amount of inorganic carbon ThIC theoretical maximum amount of inorganic carbon formed after oxidizing a chemical compound
47、 completely NOTE It is calculated from the molecular formula and expressed in this case as milligrams carbon per milligram (or gram) test compound. 3.10 lag phase time from the start of a test until adaptation and/or selection of the degrading microorganisms are achieved and the biodegradation degre
48、e of a chemical compound or organic matter has increased to about 10 % of the maximum level of biodegradation NOTE It is normally recorded in days. 3.11 maximum level of biodegradation maximum biodegradation degree of a chemical compound or organic matter in a test, above which no further biodegrada
49、tion takes place during the test NOTE It is normally recorded in percent. 3.12 biodegradation phase time from the end of the lag phase of a test until about 90 % of the maximum level of biodegradation has been reached NOTE It is normally recorded in days. 3.13 plateau phase time from the end of the biodegradation phase until the end of the test NOTE It is normally recorded in days. Licensed Copy: sheffieldun sheffieldun, na, Sat Nov