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1、Reference number ISO 3214:2000(E) ISO 2000 INTERNATIONAL STANDARD ISO 3214 Second edition 2000-07-15 Oil of Litsea cubeba (Litsea cubeba Pers.) Huile essentielle de Litsea cubeba (Litsea cubeba Pers.) Copyright International Organization for Standardization Provided by IHS under license with ISO Lic
2、ensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but sh
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5、 ISO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ISO 2000 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electro
6、nic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 ? CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.c
7、h Web www.iso.ch Printed in Switzerland ii ISO 2000 All rights reserved Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IH
8、S -,-,- ISO 3214:2000(E) ISO 2000 All rights reservediii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committee
9、s. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Inter
10、national Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodi
11、es for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights other than those identified above. ISO sh
12、all not be held responsible for identifying any or all such patent rights. International Standard ISO 3214 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 3214:1974), which has been technically revised. Annexes A and B of
13、 this International Standard are for information only. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- Copyright
14、 International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- INTERNATIONAL STANDARDISO 3214:2000(E) ISO 2000 All rights reserved1 Oil
15、of Litsea cubeba (Litsea cubeba Pers.) 1Scope ThisInternationalStandardspecifiescertain characteristics of the oil of Litsea cubeba (Litsea cubeba Pers.), in order to facilitate assessment of its quality. 2Normative references The following normative documents contain provisions which,throughreferen
16、ceinthistext,constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying th
17、e most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred toapplies.MembersofISOandIECmaintain registers of currently valid International Standards. ISO/TR 210,EssentialoilsGeneralrulesfor packaging, conditioning
18、and storage. ISO/TR 211,EssentialoilsGeneralrulesfor labelling and marking of containers. ISO 212, Essential oils Sampling. ISO 279, Essential oils Determination of relative density at 20 C Reference method. ISO 280, Essential oils Determination of refractive index. ISO 592, Essential oils Determina
19、tion of optical rotation. ISO 875, Essential oils Evaluation of miscibility in ethanol. ISO 1279, Essential oils Determination of carbonyl valuePotentiometricmethodsusing hydroxylammonium chloride. ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chroma
20、tographicprofilesforpresentationin standards. ISO 11024-2, Essential oils General guidance on chromatographicprofilesPart 2:Utilizationof chromatographic profiles of samples of essential oils. NF T 75-414, Oils of Litsea cubeba and lemongrass Determination of citral (neral + geranial) content Gas ch
21、romatographic method on packed and capillary columns. 3Term and definition For the purposes of this International Standard the following term and definition applies. 3.1 oil of Litsea cubeba essential oil obtained by steam distillation of the fresh fruits of Litsea cubeba Pers., from the Lauraceae f
22、amily NOTECAS number of oil of Litsea cubeba: 90063-59-5. 4Requirements 4.1Appearance Clear, mobile liquid. 4.2Colour Pale yellow to dark yellow. 4.3Odour Characteristic, fresh, reminiscent of citral. 4.4Relative density at 20 C, 20 20 d Minimum:0,880 0 Maximum:0,892 0 4.5Refractive index at 20 C Mi
23、nimum:1,480 0 Maximum:1,490 0 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) 2 ISO 2000 All ri
24、ghts reserved 4.6Optical rotation at 20 C Between +3 and +12. 4.7Miscibility in 70 % ethanol (volume fraction) at 20 C It shall not be necessary to use more than 3 volumes of 70 % ethanol (volume fraction) to obtain a clear solution with 1 volume of essential oil. 4.8Carbonyl index Minimum:272 This
25、corresponds to a content of carbonyl constituents, expressed as citral, of minimum:74 % 4.9Citral content (neral + geranial) by gas chromatography using the internal standard method Minimum:70 % 4.10 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In
26、 the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. Table 1 Chromatographi
27、c profile Component Minimum % Maximum % ?-Pinene1,5 Limonene915 2-Methyl-2-heptene-6-one1,83 Citronellal1,5 Linalol1,53 Neral2533 Geranial3845 Citronellol0,51,5 Nerol0,21,2 Geraniol0,51,5 NOTEThechromatographicprofileisnormative, contrary to typical chromatograms given for information in annex A. 4.
28、11 Flashpoint Information on the flashpoint is given in annex B. 5Sampling See ISO 212. Minimum volume of test sample: 30 ml. NOTEThis volume allows each of the tests specified in this International Standard to be carried out at least once. 6Test methods 6.1Relative density at 20 C, 20 20 d See ISO
29、279. 6.2Refractive index at 20 C See ISO 280. 6.3Optical rotation at 20 C See ISO 592. 6.4Miscibility in 70 % ethanol (volume fraction) at 20 C See ISO 875. 6.5Carbonyl index See ISO 1279. Test sample:1,2 g to 2 g. Standing time:15 min without heating. Relative molecular mass of citral: 152,2. 6.6Ci
30、tral content by gas chromatography See NF T 75-414. 6.7Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ
31、/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) ISO 2000 All rights reserved3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of Litsea cubeba (Litsea cub
32、eba Pers.) Peak identification 1?-Pinene 22-Methyl-2-heptene-6-one 3Limonene + 1,8-cineole 4Linalol 5Citronellal 6Neral + nerol + citronellol 7Geraniol + geranial Operating conditions Column: capillary, silica, 30 m long and of 0,25 mm internal diameter Thickness of film: not provided Stationary pha
33、se: dimethyl polysiloxane (SE 30) Oven temperature: temperature programming from 70 C to 250 C at a rate of 2 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,3 ?l Carrier gas flow rate: 1 ml/min Split ratio: 1/100
34、 NOTEWith this kind of column, neral, nerol and citronellol are not always well separated. In this case, it is recommended to carry out the analysis using a column of different polarity. Figure A.1 Typical chromatogram taken on an apolar column Copyright International Organization for Standardizatio
35、n Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) 4 ISO 2000 All rights reserved Peak identification 1?-Pinene 2Limonene 31,8-Cineole 42-Methyl-2-hepten
36、e-6-one 5Citronellal 6Linalol 7Neral 8Geranial 9Citronellol 10Nerol 11Geraniol Operating conditions Column: capillary, silica, 30 m long and of 0,25 mm internal diameter Thickness of film: not provided Stationary phase: polyethylene glycol (Supelcowax 10) cross-linked Oven temperature: temperature p
37、rogramming from 70 C to 250 C at a rate of 2 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: nitrogen Volume injected: 0,3 ?l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column Copyrigh
38、t International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) ISO 2000 All rights reserved5 Peak identification 1?-Pi
39、nene 2Limonene 31,8-Cineole 4Citronellal 5Linalol 6Neral 7Geranial 8Citronellol 9Nerol 10Geraniol Operating conditions Column: capillary, silica, 30 m long and of 0,25 mm internal diameter Thickness of film: 0,25 ?m Stationary phase: DB5TM Oven temperature: temperature programming from 60 C to 200 C
40、 at a rate of 3 C/min Injector temperature: 210 C Detector temperature: 210 C Detector: flame ionization type Carrier gas: nitrogen at 140 kPa Volume injected: 0,1 ?l Carrier gas flow rate: 0,38 m/s on the n-hexane peak Split ratio: 1/80 Figure A.3 Typical chromatogram taken on an apolar column Copy
41、right International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) 6 ISO 2000 All rights reserved Annex B (informative
42、) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (se
43、e ISO/TR 110181) concluded that it was difficulttorecommendasingleapparatusfor standardization purposes, given that: ?thereiswidevariationinthechemical composition of essential oils; ?the volume of the sample needed for certain requirements would be too costly for high-priced essential oils; ?as the
44、re are several different types of equipment which can be used for the determination, users cannot be obliged to use one specified type only. Consequently, it was decided to give a mean value for theflashpointinaninformativeannexineach International Standard, for information, in order to meet the req
45、uirements of the interested parties. The equipment with which this value was obtained should be specified. For further information see ISO/TR 110181). B.2 Flashpoint of oil of Litsea cubeba The mean value is +71 C. NOTEObtained with Luchaire equipment. _ 1)ISO/TR 11018, Essential oils General guidan
46、ce on the determination of flashpoint. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- Copyright International O
47、rganization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 3214:2000(E) ICS 71.100.60 Price based on 6 pages ISO 2000 All rights reserved Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 09:04:36 MDTNo reproduction or networking permitted without license from IHS -,-,-