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1、 Reference numbers ISO 5545:2008(E) IDF 90:2008(E) ISO and IDF 2008 INTERNATIONAL STANDARD ISO 5545 IDF 90 Second edition 2008-01-15 Rennet caseins and caseinates Determination of ash (Reference method) Casines prsure et casinates Dtermination des cendres (Mthode de rfrence) Copyright International
2、Organization for Standardization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) PDF disclaimer This PDF file may co
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6、iven below. COPYRIGHT PROTECTED DOCUMENT ISO and IDF 2008 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either
7、ISO or IDF at the respective address below. ISO copyright office International Dairy Federation Case postale 56 CH-1211 Geneva 20 Diamant Building Boulevard Auguste Reyers 80 B-1030 Brussels Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32 2 733 04 13 E-mail copyrightiso.o
8、rg E-mail infofil-idf.org Web www.iso.org Web www.fil-idf.org Published in Switzerland ii ISO and IDF 2008 All rights reserved Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008
9、 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) ISO and IDF 2008 All rights reserved iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). T
10、he work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-go
11、vernmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
12、 The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a v
13、ote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 5545IDF 90 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5,
14、Milk and milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO and IDF. This second edition of ISO 5545IDF 90 cancels and replaces the first edition (ISO 5545:1978), of which it constitutes a minor revision. Copyright International Organization for Standar
15、dization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) iv ISO and IDF 2008 All rights reserved Foreword IDF (the I
16、nternational Dairy Federation) is a non-profit organization representing the dairy sector worldwide. IDF membership comprises National Committees in every member country as well as regional dairy associations having signed a formal agreement on cooperation with IDF. All members of IDF have the right
17、 to be represented at the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO in the development of standard methods of analysis and sampling for milk and milk products. Draft International Standards adopted by the Action Teams and Standing Committees are circulated to
18、 the National Committees for voting. Publication as an International Standard requires approval by at least 50 % of the IDF National Committees casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IDF shall not be held
19、responsible for identifying any or all such patent rights. ISO 5545IDF 90 was prepared by the International Dairy Federation (IDF) and Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products. It is being published jointly by IDF and ISO. All work was carried out by th
20、e former Joint ISO-IDF-AOAC Group of Experts (E11-E701) which is now part of the Joint ISO-IDF Action Team on Physical properties and rheological tests, of the Standing Committee on Minor components and characterization of physical properties. This edition of ISO 5545IDF 90 cancels and replaces IDF
21、90:1979, of which it constitutes a minor revision. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IH
22、S -,-,- INTERNATIONAL STANDARD ISO 5545:2008(E) IDF 90:2008(E) ISO and IDF 2008 All rights reserved 1 Rennet caseins and caseinates Determination of ash (Reference method) 1 Scope This International Standard specifies a reference method for the determination of the ash of caseins obtained by rennet
23、precipitation and of caseinates, with the exception of ammonium caseinate. NOTE For the determination of ash (“fixed ash”) of acid caseins, of ammonium caseinates, of their mixtures with rennet casein and with caseinates, and of caseins of unknown type, see ISO 5544IDF 89 2. 2 Normative references T
24、he following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 3310-1, Test sieves Technical requirements and
25、 testing Part 1: Test sieves of metal wire cloth ISO 5550IDF 78, Caseins and caseinates Determination of moisture content (Reference method) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 ash of rennet caseins or of caseinates substances det
26、ermined by the procedure described in this International Standard NOTE Ash of rennet caseins or of caseinates is conventionally expressed as a percentage by mass, numerically equivalent to grams per 100 g of product. 4 Principle A test portion is incinerated at (825 25) C. The residue is then weighe
27、d. 5 Apparatus Usual laboratory apparatus, and in particular the following. 5.1 Analytical balance, capable of weighing to the nearest 0,000 1 g. 5.2 Silica or platinum dishes, of diameter about 70 mm and of depth 25 mm to 50 mm. Copyright International Organization for Standardization Provided by I
28、HS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) 2 ISO and IDF 2008 All rights reserved 5.3 Electrical furnace, with air circulati
29、on, capable of being maintained at (825 25) C. 5.4 Desiccator, containing an effective desiccant. 5.5 Grinding device, for grinding the laboratory sample, if necessary (see 7.1.4), without development of undue heat and without loss or absorption of moisture. A hammer-mill shall not be used. 5.6 Test
30、 sieve, wire cloth, diameter 200 mm, nominal size of aperture 500 m, with receiver, complying with ISO 3310-1. 6 Sampling A representative sample should have been sent to the laboratory. It should not have been damaged or changed during transport or storage. Sampling is not part of the method specif
31、ied in this International Standard. A recommended sampling method is given in ISO 707|IDF 50 1. 7 Procedure 7.1 Preparation of the test sample 7.1.1 Thoroughly mix the laboratory sample by repeatedly shaking and inverting the container (if necessary, after having transferred all of the laboratory sa
32、mple to an airtight container of sufficient capacity to allow this operation to be carried out). 7.1.2 Transfer about 50 g of the thoroughly mixed laboratory sample to the test sieve (5.6). 7.1.3 If the 50 g portion directly passes or almost completely passes the sieve, use for the determination the
33、 sample as prepared in 7.1.1. 7.1.4 Otherwise, grind the 50 g portion, using the grinding device (5.5), until it passes the sieve. Immediately transfer all of the sieved sample to an airtight container of sufficient capacity and mix thoroughly by repeatedly shaking and inverting. During these operat
34、ions, take precautions to avoid any change in the water content of the product. 7.1.5 After the test sample has been prepared, the determination (7.4) should proceed as soon as possible. Clean the device after grinding each sample. 7.2 Preparation of the dish Heat the dish (5.2) in the electrical fu
35、rnace (5.3), maintained at 825 C, for 30 min. Allow the dish to cool in the desiccator (5.4) to the temperature of the balance room and weigh to the nearest 0,1 mg. 7.3 Test portion Weigh, to the nearest 0,1 mg, directly in or by difference approximately 3,000 0 g of the test sample (7.1) into the p
36、repared dishes. 7.4 Determination Heat the dish containing the test portion on a low flame until the test portion is completely charred, taking care that it does not burst into flame. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=IHS Employe
37、es/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) ISO and IDF 2008 All rights reserved 3 Transfer the dish to the electrical furnace (5.3), maintained at (825 25) C, and heat fo
38、r at least 1 h until all carbon has disappeared from the dish. Allow the dish to cool in the desiccator (5.4) to the temperature of the balance room and weigh to the nearest 0,1 mg. Repeat the operations of heating in the electrical furnace (5.3), cooling and weighing, until the mass remains constan
39、t to within 1 mg or begins to increase. Record the minimum mass. 8 Expression of results 8.1 Calculation 8.1.1 The ash of the sample, wa, as a percentage by mass, is given by Equation (1): mm w m = 12 a 0 100 (1) where m0 is the mass, in grams, of the test portion (7.3); m1 is the mass, in grams, of
40、 dish and residue (7.4); m2 is the mass, in grams, of the prepared dish (7.2). Calculate the ash to the nearest 0,01 % by mass and report the final result to the nearest 0,1 % by mass. 8.1.2 To calculate the ash of the sample on the dry basis, as a percentage by mass, multiply the result obtained fr
41、om Equation (1) by Factor (2): w w 100 100 (2) where ww is the water content, as a percentage by mass, of the sample determined according to ISO 5550IDF 78. 8.2 Precision 8.2.1 Repeatability The absolute difference between two independent single test results, obtained using the same method on identi
42、cal test material in the same laboratory by the same operator using the same equipment within a short interval of time will in not more than 5 % of cases be greater than 0,15 g of ash per 100 g of product (numerically equivalent to 0,15 % by mass). 8.2.2 Reproducibility The absolute difference betwe
43、en two independent single test results, obtained using the same method on identical test material in different laboratories with different operators using different equipment, will in not more than 5 % of cases be greater than 0,25 g of ash per 100 g of product (numerically equivalent to 0,25 % by m
44、ass). Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) 4 ISO
45、 and IDF 2008 All rights reserved 9 Test report The test report shall specify: a) all the information required for the complete identification of the sample; b) the sampling method used, if known; c) the test method used, together with a reference to this International Standard; d) all operating det
46、ails not specified in this International Standard, or regarded as optional, together with details of any incident that may have influenced the result(s); e) the test result(s) obtained, and if the repeatability has been checked, the final quoted results obtained. Copyright International Organization
47、 for Standardization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,- ISO 5545:2008(E) IDF 90:2008(E) ISO and IDF 2008 All rights reserved 5 Bibliogr
48、aphy 1 ISO 707|IDF 50, Milk and milk products Guidance on sampling 2 ISO 5544IDF 89, Caseins Determination of “fixed ash” (Reference method) Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=IHS Employees/1111111001, User=Japan, IHS Not for Resale, 02/16/2008 02:13:01 MSTNo reproduction or networking permitted without license from IHS -,-,